Abstract: A method for controlling a flow-rate of fluid flowing in a microfluidic network comprising a microchannel and a plurality of inlet/outlet interface ports is disclosed. The method includes: successively applying a plurality of pressures on at least one inlet/outlet interface port, measuring a time series of flow-rate values of the fluid generated in response to the plurality of applied pressures, estimating parameters of a model of the microfluidic network response to input pressure values based on the applied pressure values and the measured time series of output flow-rate values, computing a target pressure value at each of the inlet/outlet interface ports corresponding to a predetermined flow-rate value at the flow-rate measuring point, wherein the predetermined flow-rate value corresponds to an output value of the model of the microfluidic network response to the target pressure value, and applying the computed target pressure value on the at least one inlet/outlet interface port.