Patents by Inventor Stéphanie Aquin
Stéphanie Aquin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170247714Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: ApplicationFiled: March 30, 2017Publication date: August 31, 2017Applicants: Medicago, Inc., Centre National de la Recherche Scientifique, Universite De RouenInventors: Thomas PACCALET, Muriel BARDOR, Christophe RIHOUEY, Véronique GOMORD, Loïc FAYE, Patrice LEROUGE, Stéphanie AQUIN, Louis-Philippe VEZINA, Marc-André D'AOUST
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Patent number: 9657305Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: GrantFiled: November 4, 2015Date of Patent: May 23, 2017Assignees: Medicago Inc., Centre National de la Recherche Scientifique, Universite De RouenInventors: Thomas Paccalet, Muriel Bardor, Christophe Rihouey, Véronique Gomord, Loïc Faye, Patrice Lerouge, Stéphanie Aquin, Louis-Philippe Vezina, Marc-André D'Aoust
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Publication number: 20160145664Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: ApplicationFiled: November 4, 2015Publication date: May 26, 2016Applicants: Medicago Inc., Centre National de la Recherche Scientifique, Universite De RouenInventors: Thomas PACCALET, Muriel BARDOR, Christophe RIHOUEY, Véronique GOMORD, Loïc FAYE, Patrice LEROUGE, Stéphanie AQUIN, Louis-Philippe VEZINA, Marc-André D'AOUST
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Patent number: 9212372Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: GrantFiled: February 9, 2007Date of Patent: December 15, 2015Assignees: Medicago Inc., Centre National de la Recherche Scientifique, Université De RouenInventors: Thomas Paccalet, Muriel Bardor, Christophe Rihouey, Véronique Gomord, Loïc Faye, Patrice Lerouge, Stéphanie Aquin, Louis-Philippe Vezina, Marc-André D'Aoust
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Publication number: 20110008837Abstract: A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.Type: ApplicationFiled: June 13, 2008Publication date: January 13, 2011Inventors: Marc-Andre D-Aoust, Louis-Philippe Vezina, Stephanie Aquin, Estelle Marquet-Blouin, Muriel Bardor, Veronique Gomord, Christophe Rihouey, Thomas Paccalet, Carole Burel, Loic Faye, Patrice Lerouge, Christophe Sourrouille
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Patent number: 7786355Abstract: Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.Type: GrantFiled: November 16, 2001Date of Patent: August 31, 2010Assignee: Metabolix, Inc.Inventors: Stephanie Aquin, Oliver P. Peoples, Kristi D. Snell
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Publication number: 20090312240Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated a protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, CMP-Neu5Ac transporter and a sialyltransferase.Type: ApplicationFiled: February 9, 2007Publication date: December 17, 2009Inventors: Thomas Paccalet, Muriel Bardor, Chirstophe Rihouey, Véronique Gomord, Loïc Faye, Patrice Lerouge, Stéphanie Aquin, Louis-Philippe Vezina, Marc-André D'Aoust
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Publication number: 20030017576Abstract: Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.Type: ApplicationFiled: November 16, 2001Publication date: January 23, 2003Inventors: Stephanie Aquin, Oliver P. Peoples, Kristi D. Snell
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Patent number: 6492134Abstract: The present biotechnological approach for the production of polyhydroxyalkanotes (PHAs) uses microbial systems. The high production costs makes them substantially more expensive than synthetic plastics. Engineering a novel pathway in eucaryotic cell systems is a beneficial alternative to the production of PHAs in bacteria. This pathway will initially produce free (C8) fatty acids from the fatty acid synthetic pathway through the action of thioesterase, that will then add a CoA moiety to the free fatty acid through the action of an acyl-CoA synthetase, that will produce 3-ketoacyl-CoAs from the acyl-CoA through the action of a thiolase, that will produce R-(−)-OH-acyl-CoAs from the 3-keto acid CoAs through the action of a dehydrogenase isoform from yeasts. These R-(−)-3-OH-acyl-CoAs will finally be used as substrate for the PHA synthase reaction.Type: GrantFiled: March 15, 2000Date of Patent: December 10, 2002Assignees: Université Laval, Her Majesty the Queen in right of Canada as represented by the Minister of Agriculture and Agri-Food CanadaInventors: Stéphanie Aquin, Louis-P. Vézina