Abstract: A cassette is configured to contain a microfluidic chip. The cassette includes a base made of rigid material and provided with a first wall. The cassette further includes a cover made of rigid material and provided with a second wall. The cassette has an analysis position and an introduction position. Each of the first wall and the second wall has an optically transparent viewing zone. The cassette further includes a series of connecting orifices, each orifice of the series of connecting orifices being designed to be passed through by a connecting tube allowing the passage of a fluid. The second wall also comprising at least one sample introduction orifice.

Abstract: Plate accessory for a microfluidic experimentation device comprising a first part and at least a second part, said second part being detachable from said first part, said first part having a plurality of through-holes arranged to allow the fluidic connection tubings to pass through said first part, said second part having: at least one housing for at least one microfluidic chip dug into in the thickness of said second part and which has a hole for the passage of light, a plurality of holes, each hole being arranged to receive a fluid reservoir.

Abstract: A microfluidic device for use in field of droplet microfluidics is disclosed. The microfluidic device can manipulate a discrete element, for example a droplet. The discrete element may include a medium and a component. The microfluidic device may include a main microfluidic channel, some stopping elements and an attractive mechanism that may retain, physically and in a releasable way, the component at a given location in the main microfluidic channel. The discrete element may be split into a first and second parts in such a way that the component ends in the second parts. The microfluidic device. may be used especially for a single-cell analysis.

Abstract: A microfluidic module for co-encapsulation in droplets of two populations of particles may include first and second modules for sorting the two populations. The modules may have their first outlets including first obstructive valves configured to at least partially obstruct the first outlets. The first outlets may be fluidly connected to a fusion module, including a fusion module means for merging at least one droplet from the first droplet population and at least one droplet from the second droplet population into a merged droplet comprising the two population of particles, and a control unit for controlling the first obstructive valves from information originating from a first and second module detection portion located upstream of the first outlets.

Abstract: A microfluidic module for co-encapsulation in droplets of two populations of particles may include first and second modules for sorting the two populations. The modules may have their first outlets including first obstructive valves configured to at least partially obstruct the first outlets. The first outlets may be fluidly connected to a fusion module, including a fusion module means for merging at least one droplet from the first droplet population and at least one droplet from the second droplet population into a merged droplet comprising the two population of particles, and a control unit for controlling the first obstructive valves from information originating from a first and second module detection portion located upstream of the first outlets.