Abstract: A method and device for determining pancreatic lipase in an animal blood sample. In one aspect, the device includes a compartmentalized container for storing and reacting the reagents for use in the assay. In another aspect, the device include a multilayer device having a spreading layer and reagents layers with dried reagents for detecting pancreatic lipase. The devices also includes a system for the optical determination of a color change in the reaction mixture. The method includes the use of the device to detect pancreatic lipase in samples.

Abstract: Isolated nucleic acid molecules having a nucleotide sequence encoding canine pancreatic lipase polypeptides, allelic variants and fragments thereof. Vectors and host cells containing the polynucleotide sequences and methods for expressing the polypeptides. Monoclonal antibodies that specifically binds to the canine pancreatic lipase polypeptides. Cell lines secreting the monoclonal antibodies. Methods for determining the presence or amount of canine pancreatic lipase in a biological sample. The methods include using the monoclonal antibodies to specifically bind to canine pancreatic lipase polypeptides. The method includes using standards of recombinant canine pancreatic lipase. Devices and kits for performing methods for detecting canine pancreatic lipase in biological samples.