Abstract: Systems and methods for accurately measuring changes in biomarker sensitive hydrogel volume and shape due to exposure to various biomarkers include a system for identifying one or more dimensional changes in a biomarker sensitive hydrogel positioned within an in vivo environment. The system includes a biomarker sensitive hydrogel positioned within an in vivo environment and configured to dimensionally change in response to interaction with predefined biomarkers. The system additionally includes an ultrasound transducer for locating and identifying one or more characteristics of the biomarker sensitive hydrogel and a computer system in electrical communication with the ultrasound transducer. The computer system is configured to receive characteristics of the biomarker sensitive hydrogel from the ultrasound transducer and determine dimensional changes of the biomarker sensitive hydrogel based on the received characteristics.

Abstract: Systems, methods, and sensor devices for identifying one or more changes in a stimulus-responsive hydrogel include a sensor device having (i) a sensing structure and (ii) a stimulus-responsive hydrogel associated with a first side of the sensing structure. The sensing structure includes a flexible thin film polymer and an electric sensing element capable of electric impedance change, and the hydrogel is configured to dimensionally change in response to predefined stimuli such that a dimensional change of the hydrogel causes a change in an impedance property of the electric sensing element. Systems including such a sensor device can additionally include a meter in electrical communication with the sensor device to identify changes in the impedance properties of the structure and/or a catheter sheath configured for placement within an in vivo environment and is sized and shaped to receive the sensor device within a lumen thereof.

Abstract: A super resolution microscope system is disclosed and described. The system can include a sample stage (180) adapted to receive a sample (185) including probe molecules. At least one light source (105) is provided to produce a coherent excitation light to excite the probe molecules and cause luminescence of the probe molecules. An image detector (100) can detect the luminescence from the probe molecules. A microlens array (125) can be positioned in a beam path (110) of the coherent light from the at least one light source (105). The beam path (110) of the coherent light extends between the light source (105) and the sample stage (180). The microlens array (125) can also be positioned in a beam path (112) of the luminescence from the probe molecules. The beam path (112) of the luminescence extends between the sample stage (180) and the image detector (100).

Abstract: Systems and methods for accurately measuring changes in biomarker sensitive hydrogel volume and shape due to exposure to various biomarkers include a system for identifying one or more dimensional changes in a biomarker sensitive hydrogel positioned within an in vivo environment. The system includes a biomarker sensitive hydrogel positioned within an in vivo environment and configured to dimensionally change in response to interaction with predefined biomarkers. The system additionally includes an ultrasound transducer for locating and identifying one or more characteristics of the biomarker sensitive hydrogel and a computer system in electrical communication with the ultrasound transducer. The computer system is configured to receive characteristics of the biomarker sensitive hydrogel from the ultrasound transducer and determine dimensional changes of the biomarker sensitive hydrogel based on the received characteristics.

Abstract: A super resolution microscope system is disclosed and described. The system can include a sample stage (180) adapted to receive a sample (185) including probe molecules. At least one light source (105) is provided to produce a coherent excitation light to excite the probe molecules and cause luminescence of the probe molecules. An image detector (100) can detect the luminescence from the probe molecules. A microlens array (125) can be positioned in a beam path (110) of the coherent light from the at least one light source (105). The beam path (110) of the coherent light extends between the light source (105) and the sample stage (180). The microlens array (125) can also be positioned in a beam path (112) of the luminescence from the probe molecules. The beam path (112) of the luminescence extends between the sample stage (180) and the image detector (100).

Abstract: A system and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage. The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected linearly via an sCMOS camera. Object planes in corresponding recorded regions of interest are recorded in the camera. A signal from the regions of interest is combined into a three dimensional image.

Abstract: A method is described for correlating microscopy images from a number of modalities in a sub diffraction resolution environment. The method may include receiving a number of datasets that may represent microscopy captures from a number of different modalities. The microscopy captures may contain feature markers that may be used to register a number of data points contained in a dataset with data points from another dataset. Upon registering the data points of the datasets, a combined dataset may be produced and a visual image of the combined dataset may be provided.

Abstract: A system and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage. The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected linearly via an sCMOS camera. Object planes in corresponding recorded regions of interest are recorded in the camera. A signal from the regions of interest is combined into a three dimensional image.

Abstract: A method is described for correlating microscopy images from a number of modalities in a sub diffraction resolution environment. The method may include receiving a number of datasets that may represent microscopy captures from a number of different modalities. The microscopy captures may contain feature markers that may be used to register a number of data points contained in a dataset with data points from another dataset. Upon registering the data points of the datasets, a combined dataset may be produced and a visual image of the combined dataset may be provided.

Abstract: A correlative drift correction system can include a sample stage for supporting a sample and a cover slip. The system can include an infrared light source for emitting infrared light to be reflected at the cover slip and an optical sensor for detecting the reflected infrared light. The system can detect drift of the sample using reflected infrared light data from the optical sensor and can determine a drift correction to apply to image data of the sample.