Patents by Inventor Stanislas Dusko Ehrlich

Stanislas Dusko Ehrlich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for in vivo production of a mutant library in cells
    Patent number: 6500644
    Abstract: A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises i) an origin of replication from which replication is initiated, ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic, iii) a gene encoding the polypeptide of interest. Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.
    Type: Grant
    Filed: September 26, 2000
    Date of Patent: December 31, 2002
    Assignee: Novozymes A/S
    Inventors: Torben Vedel Borchert, Stanislas Dusko Ehrlich
  • ppGpp synthetase and expression systems for improved production of protein of interest
    Publication number: 20020119551
    Abstract: The present invention relates to a novel ppGpp synthetase and expression systems for improved production of proteins of interest in Gram-positive bacteria, involving use of the novel ppGpp synthetase.
    Type: Application
    Filed: September 5, 2001
    Publication date: August 29, 2002
    Applicant: Novozymes A/S
    Inventors: Jens Tonne Andersen, Stanislas Dusko Ehrlich
  • In vivo recombination
    Patent number: 6326206
    Abstract: The present invention relates to a method for in vivo recombination of homologous DNA sequences. The method is a forced artificial evolution resulting in a DNA sequence encoding a polypeptide having an advantageous property.
    Type: Grant
    Filed: June 10, 1999
    Date of Patent: December 4, 2001
    Assignee: Novozymes A/S
    Inventors: Mads Eskelund Bjornvad, Michael Dolberg Rasmussen, Per Lina Jorgensen, Torben Vedel Borchert, Stanislas Dusko Ehrlich
  • PPGPP synthetase and expression systems for improved production of protein of interest
    Patent number: 6306631
    Abstract: The present invention relates to a novel ppGpp synthetase and expression systems for improved production of proteins of interest in Gram-positive bacteria, involving use of the novel ppGpp synthetase.
    Type: Grant
    Filed: November 16, 1999
    Date of Patent: October 23, 2001
    Assignee: Novozymes A/S
    Inventors: Jens Tonne Andersen, Stanislas Dusko Ehrlich
  • Method for in vivo production of a mutant library in cells
    Patent number: 6165718
    Abstract: A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprisesi) an origin of replication from which replication is initiated,ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic,iii) a gene encoding the polypeptide of interest.Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.
    Type: Grant
    Filed: July 8, 1998
    Date of Patent: December 26, 2000
    Assignee: Novo Nordisk A/S Novo Alle
    Inventors: Torben Vedel Borchert, Stanislas Dusko Ehrlich