Abstract: The invention related to forming nucleic add templates including control templates for sequencing using a nanopore-based method, wherein the templates of the novel structure disclosed herein are limited or prevented from threading into the nanopore during sequencing.

Abstract: A video monitoring system and method comprises a first camera at a first camera location configured to video record a first monitored area, wherein a georeference location of the first monitored area is known, and a virtual 3D model of the monitored zone that includes the first camera location and the first monitored area, wherein the virtual 3D model is georeferenced to a physical location. The system further comprises a processor configured to receive the video recording of the first monitored area and to overlay the video recording of the first monitored area onto a part of the virtual 3D model that corresponds to the georeference location of the first monitored area. A display is also connected to the processor and configured to display the virtual 3D model of the monitored zone overlaid with the video recording of the first monitored area.

Abstract: Silicone oil is a reaction medium or separation medium, or both, for ester synthesis. Enzyme-catalyzed esterification is conducted in the silicone oil, with ester product soluble therein. Ester is separated from silicone by distillation. Esterification in silicone oil in conjunction with a simultaneous fermentation process, results in alcohol fermentation products flowing through the intervening silicone oil (serving as an oxygen barrier) to react with organic acid at an oil:aqueous interface, catalyzed by an enzyme (e.g., lipase, esterase). Ester solubility in silicone is leveraged for extraction, isolating catalyzed (metal, chemical or enzyme) ester reaction products from an original reaction (aqueous or organic) medium. Esters may be generated from alcohol fermented from organic matter of agricultural waste products, utilizing cheaply available silicone oil. Similarly, silicone oil may also be used to extract from a polar solvent (e.g.

Abstract: A video monitoring system and method comprises a first camera at a first camera location configured to video record a first monitored area, wherein a georeference location of the first monitored area is known, and a virtual 3D model of the monitored zone that includes the first camera location and the first monitored area, wherein the virtual 3D model is georeferenced to a physical location. The system further comprises a processor configured to receive the video recording of the first monitored area and to overlay the video recording of the first monitored area onto a part of the virtual 3D model that corresponds to the georeference location of the first monitored area. A display is also connected to the processor and configured to display the virtual 3D model of the monitored zone overlaid with the video recording of the first monitored area.