Abstract: An object is to make a bridge of DNA expanding between a pair of electrodes, and to characterize the bridge of DNA, to thereby detect DNA easily and surely without employing any marker or labeling substances, such as fluorescent reagents. A method of detecting DNA using a detection device with at least a couple of electrodes, the method comprising immobilizing a primer on the electrodes; making a bridge of the DNA expanded between the electrodes, by immersing the electrodes in a solution including circular templates of single stranded DNA, annealing the circular templates, and generating single stranded DNA product utilizing RCA (Rolling Circle Amplification), with impressing a designated voltage between the electrodes; and characterizing the bridge of DNA which includes multiple single stranded DNA molecules between the electrodes.

Abstract: An object is to make a bridge of DNA expanding between a pair of electrodes, and to characterize the bridge of DNA, to thereby detect DNA easily and surely without employing any marker or labeling substances, such as fluorescent reagents. A method of detecting DNA using a detection device with at least a couple of electrodes, the method comprising immobilizing a primer on the electrodes; making a bridge of the DNA expanded between the electrodes, by immersing the electrodes in a solution including circular templates of single stranded DNA, annealing the circular templates, and generating single stranded DNA product utilizing RCA (Rolling Circle Amplification), with impressing a designated voltage between the electrodes; and characterizing the bridge of DNA which includes multiple single stranded DNA molecules between the electrodes.

Abstract: Methods for tissue microdissection- or dissociation-free means of collecting cell-specific biomolecules from complex heterogeneous tissue sources including native tissues, fresh frozen, fixed, and archived specimens. The tissue section having a plurality of beads with immobilized capture probes on their surface evenly distributed over all cell types of the tissue is arranged. Alternatively the tissue section being coated with a thin layer of resin containing incorporated or covalently bound immobilizing capture probes is arranged. The beads or resin are incubated directly on the tissue section permitting capturing of the biomolecules by the capture probes. After incubation, the tissue section is visualized and the cells or area of interest are identified. Next, the beads or resin samples, located in these cells or areas of interest, are collected and transferred to a sample tube where biomolecules are separated from the beads or resin using standard methods and are then used in downstream applications.

Abstract: The present invention relates to a capillary-based cell and tissue acquisition system that integrates the capillary approach with a microscope manipulator to collect and sort cells of interest. Cells of interest are determined by using a laser beam focus to identify the initial contact between the capillary and the cells.

Abstract: A sampling apparatus for collection and preservation of live cells comprising a collection tube, a capillary tube extending distally and substantially axially from the collection tube, the capillary tube having a first capillary tube section defining a first capillary tube inside diameter, and a luer hub engaged proximally and substantially axially on the collection tube substantially opposite the capillary tube, the luer hub having a luer passage formed therethrough, whereby installation of the sampling apparatus in a linear actuator assembly capable of providing a vacuum as by engaging the luer hub with a filter coupling of the linear actuator assembly enables selective suctioning through the luer passage, the collection tube, and the capillary tube so as to collect one or more cells within the collection tube of the sampling apparatus.

Abstract: A protein detection device and method. The device has a main channel in which is attached either a polar rail molecule or a biomolecular motor which moves on the rail molecule in a direction corresponding to the polarity of the rail molecule. A sub-channel crosses the main channel for receiving a sample including tau-protein. The method includes feeding the sample to the device and detecting a ratio of mutated tau-protein included in the sample, based on motion of the biomolecular motor on the rail molecule or on motion of the rail molecule moved by action of the biomolecular motor.

Abstract: The present invention relates to a capillary-based cell and tissue acquisition system that integrates the capillary approach with a microscope manipulator to collect and sort cells of interest. Cells of interest are determined by using a laser beam focus to identify the initial contact between the capillary and the cells.