Patents by Inventor Stanislaw Flasinski

Stanislaw Flasinski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10316331
    Abstract: The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: June 11, 2019
    Assignee: Monsanto Technology LLC
    Inventors: David J. Bowen, Catherine Chay, Artem Evdokimov, Stanislaw Flasinski, Uma R. Kesanapalli, Megan N. Schroder, Rachael N. Slightom, Nengbing Tao, Andrew M. Wollacott
  • Patent number: 10301625
    Abstract: The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: May 28, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Stanislaw Flasinski, Charles R. Dietrich, Wei Wu, Zhaolong Li, Bo-Xing Qiu, Liang Guo, Jaishree M. Chittoor
  • Patent number: 10287605
    Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: May 14, 2019
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Thomas Cerruti, Stanislaw Flasinski, Xiaoran Fu, Arlene R. Howe, Sara Ann Salvador
  • Patent number: 10233217
    Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: March 19, 2019
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Sara A. Salvador, Jinling Wang, Stanislaw Flasinski, Thomas A. Cerruti, Crystal L. Dart, Leigh H. English, Xiaoran Fu, Victor M. Guzov, Arlene R. Howe, Jay P. Morgenstern, James K. Roberts
  • Patent number: 10227599
    Abstract: The present invention provides regulatory polynucleotide molecules isolated from plant proline rich protein genes and linked to a viral enhancer molecule. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the regulatory polynucleotide sequences, and methods for preparing and using the same.
    Type: Grant
    Filed: July 28, 2016
    Date of Patent: March 12, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Stanislaw Flasinski, Shirley Xiaoli Guo, Wei Wu
  • Patent number: 10208314
    Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: February 19, 2019
    Assignee: Monsanto Technology LLC
    Inventor: Stanislaw Flasinski
  • Publication number: 20190024111
    Abstract: The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.
    Type: Application
    Filed: July 30, 2018
    Publication date: January 24, 2019
    Inventors: Jaishree M. Chittoor, Stanislaw Flasinski, Mohammed Oufattole, Michael W, Petersen
  • Publication number: 20180346925
    Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed, and includes, but is not limited to, TIC5290. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC5290 pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC5290 pesticidal protein of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the TIC5290 pesticidal protein are also provided.
    Type: Application
    Filed: June 7, 2018
    Publication date: December 6, 2018
    Inventors: David J. Bowen, Catherine A. Chay, Stanislaw Flasinski, Yong Yin
  • Publication number: 20180334684
    Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.
    Type: Application
    Filed: July 27, 2018
    Publication date: November 22, 2018
    Applicant: Monsanto Technology LLC
    Inventors: James A. Baum, Thomas Cerruti, Stanislaw Flasinski, Xiaoran Fu, Arlene R. Howe, Sara Ann Salvador
  • Patent number: 10100330
    Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed, and includes, but is not limited to, TIC5290. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC5290 pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC5290 pesticidal protein of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the TIC5290 pesticidal protein are also provided.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: October 16, 2018
    Assignee: Monsanto Technology LLC
    Inventors: David J. Bowen, Catherine A. Chay, Stanislaw Flasinski, Yong Yin
  • Publication number: 20180291395
    Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention.
    Type: Application
    Filed: April 3, 2018
    Publication date: October 11, 2018
    Inventors: David J. Bowen, Catherine A. Chay, Todd A. Ciche, Stanislaw Flasinski, Arlene R. Howe, Krishnakumar Sridharan
  • Patent number: 10059959
    Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: August 28, 2018
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Thomas Cerruti, Stanislaw Flasinski, Xiaoran Fu, Arlene R. Howe, Sara Ann Salvador
  • Patent number: 10059954
    Abstract: The invention provides novel recombinant DNA molecules and constructs useful for modulating gene expression in plants, plant cells, seeds, and progeny plants. Plant regulatory elements comprising sequences from a ubiquitin gene from Bouteloua gracilis, as well as variants and fragments thereof having gene-regulatory activity, are provided. The invention also provides transgenic plants, plant cells, plant parts, seeds, and progeny plants comprising the recombinant DNA molecules of the invention, along with methods of their use.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: August 28, 2018
    Assignee: Monsanto Technology LLC
    Inventor: Stanislaw Flasinski
  • Publication number: 20180208940
    Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.
    Type: Application
    Filed: December 19, 2017
    Publication date: July 26, 2018
    Inventors: David J. Bowen, Catherine A. Chay, Todd A. Ciche, Stanislaw Flasinski, Arlene R. Howe, Jason S. Milligan, James K. Roberts, Yong Yin
  • Publication number: 20180208631
    Abstract: Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.
    Type: Application
    Filed: January 11, 2018
    Publication date: July 26, 2018
    Inventors: James A. Baum, David J. Bowen, Catherine A. Chay, David J. Chi, William P. Clinton, Crystal L. Dart, Leigh English, Stanislaw Flasinski, Victor M. Guzov, Kevin A. Jarrell, Uma R. Kesanapalli, Thomas M. MaIvar, Robert M. McCarroll, Jason S. Milligan, Jay P. Morgenstern, Deborah G. Rucker, Sara A. Salvador, Temple F. Smith, Carlos E. Soto, Collin M. Stultz, Brian M. Turczyk, Ty T. Vaughn, Moritz W.F.F. Von Rechenberg
  • Patent number: 10017549
    Abstract: A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.
    Type: Grant
    Filed: July 25, 2013
    Date of Patent: July 10, 2018
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Stanislaw Flasinski, Gregory R. Heck, Stephen R. Penn, Uma Rao Sukuru, Xiaohong Shi
  • Publication number: 20180148731
    Abstract: The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.
    Type: Application
    Filed: November 3, 2017
    Publication date: May 31, 2018
    Inventors: Stanislaw Flasinski, Barrett C. Foat, Mohammed Oufattole, Randall W. Shultz, Xiaoping Wei, Wei Wu, Shiaw-Pyng Yang
  • Patent number: 9974302
    Abstract: The present invention provides novel insecticidal proteins active against a Coleopteran and/or Hemipteran species pest, which include, but are not limited to, TIC3131, TIC3400 and TIC3407 proteins. The present invention also provides a DNA construct comprising operably linked to a heterologous promoter a polynucleotide that encodes the novel insecticidal protein or an insecticidal fragment thereof. The present invention further provides transgenic plants/plant cells/plant parts expressing the insecticidal proteins, methods for detecting the presence of the polynucleotide or the protein in a biological sample, and methods of controlling a Coleopteran and/or Hemipteran species pest using the insecticidal proteins.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: May 22, 2018
    Assignee: Monsanto Technology LLC
    Inventors: Heather M. Anderson, David J. Bowen, Catherine A. Chay, Stanislaw Flasinski, Uma R. Kesanapalli, Jason S. Milligan, Rachael N. Slightom, Yong Yin
  • Publication number: 20180127772
    Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.
    Type: Application
    Filed: December 20, 2017
    Publication date: May 10, 2018
    Applicant: Monsanto Technology LLC
    Inventors: James A. Baum, Thomas A. Cerruti, Crystal L. Dart, Leigh H. English, Stanislaw Flasinski, Xiaoran Fu, Victor M. Guzov, Arlene R. Howe, Jay P. Morgenstern, James K. Roberts, Sara A. Salvador, Jinling Wang
  • Publication number: 20180127773
    Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.
    Type: Application
    Filed: December 20, 2017
    Publication date: May 10, 2018
    Applicant: Monsanto Technology LLC
    Inventors: James A. Baum, Thomas A. Cerruti, Crystal L. Dart, Leigh H. English, Stanislaw Flasinski, Xiaoran Fu, Victor M. Guzov, Arlene R. Howe, Jay P. Morgenstern, James K. Roberts, Sara A. Salvador, Jinling Wang