Abstract: Haemophilus adhesion and penetration proteins, nucleic acids, vaccines and monoclonal antibodies are provided.

Abstract: Haemophilus adhesion and penetration proteins, nucleic acids, vaccines and monoclonal antibodies are provided.

Abstract: Haemophilus adhesion and penetration proteins, nucleic acids, vaccines and monoclonal antibodies are provided.

Abstract: Haemophilus adhesion and penetration proteins, nucleic acids, vaccines and monoclonal antibodies are provided.

Abstract: Haemophilus adhesion and penetration proteins, nucleic acids, vaccines and monoclonal antibodies are provided.

Abstract: Three classes of GFP mutants having single excitation maxima around 488 nm are brighter than wild-type GFP following 488 nm excitation. GFPmut1 has a double substitution: F64L, S65T; GFPmut2 has a triple substitution: S65A, V68L, S72A; and GFPmut3 is characterized by the double substitution S65G, S72A. The excitation maxima of the three mutants are at 488 nm, 481 nm and 501 nm respectively. The fluorescence intensities following excitation at 488 nm are an order of magnitude higher than that of wild-type GFP excited at 488 nm in E. coli. The expression of GFP is observable minutes after induction.

Abstract: Nucleic acid and protein compositions are provided from B. pertussis which may find use in diagnosis, prevention and therapy of whooping cough. Particularly, an open reading frame encoding filamentous hemagglutinin precursors provided, with the intact protein for the filamentous hemagglutinin portion thereof, can be expressed in a wide variety of hosts, for use in the production of antibodies, for immunodiagnosis or therapy, or as vaccines for prophylactic purposes.

Abstract: Regulatory elements (e.g. promoters) activated by a stimulus are isolated by a FACS-based method. Preferably, a library of random fragments representative of a target (e.g. bacterial) genome are cloned in front of a promoterless gfp (green fluorescent protein) sequence in a plasmid, and inserted into target cells. The resulting target cell mixture is sorted according to GFP levels in the presence and the absence of the stimulus. Suitable stimuli include compounds of interest (e.g. drugs), environmental factors (e.g. extracellular acidity), and complex stimuli such as in vivo environments of hosts infected by the target cells. The method allows identifying pathogen genes which are selectively expressed during infection.

Abstract: Three classes of GFP mutants having single excitation maxima around 488 nm are brighter than lid-type GFP following 488 nm excitation. GFPmut1 has a double substitution: F64L, S65T; GFPmut2 has a triple substitution: S65A, V68L, S72A; and GFPmut3 is characterized by the double substitution S65G, S72A. The excitation maxima of the three mutants are at 488 nm, 481 nm and 501 nm respectively. The fluorescence intensities following excitation at 488 nm are an order of magnitude higher than that of wild-type GFP excited at 488 nm in E. coli. The expression of GFP is observable minutes after induction.

Abstract: Novel methods and microorganisms are provided, where novel genetic mammalian cell invasive capability is imparted to a microorganism by the introduction of an exogenous ail or hil gene. The resulting organisms are then capable of binding to mammalian cells and are transferred to the cytoplasm. Other novel genetic capabilities may be imparted to the unicellular microorganism, which may serve as a vaccine for one or more pathogens or may introduce genetic capabilities or foreign molecules into a mammalian host cell. The sequences may be used for an in vitro screen for pathogenicity. Mutant microorganisms having an attenuated invasive phenotype are also disclosed wherein one or more invasive genes have been modified.

Abstract: The invention provides nucleic acids encoding one or more hyper-invasive genes within the hil locus (hyper-invasion locus) or fragments thereof, methods for making attenuated microorganisms and identifying such hyper-invasive nucleic acids as well as mutant microorganisms wherein one or more hyper-invasive genes within the hil locus are modified to attenuate the invasive phenotype of the microorganism. The methods of the invention utilize conditions which repress invasiveness in an otherwise invasive microorganism. The method comprises mutating an invasive microorganism to form a plurality of mutant microorganisms. The thus formed mutants are exposed to conditions which repress invasiveness of the parental invasive microorganism. At least one mutant microorganism is then detected which exhibits an increase in invasiveness as compared to the parental invasive microorganism.

Abstract: The invention relates to yeast leukotriene A.sub.4 hydrolase enzymes and nucleic acids.

Abstract: Novel methods and microorganisms are provided, where novel genetic mammalian cell invasive capability is imparted to a microorganism by the introduction of an exogenous inv gene. The resulting organisms are then capable of binding to mammalian cells and are transferred to the cytoplasm. Other novel genetic capabilities may be imparted to the unicellular microorganism, which may serve as a vaccine for one or more pathogens or may introduce genetic capabilities or foreign molecules into a mammalian host cell. The sequences may be used for an in vitro screen for pathogenicity.

Abstract: Novel methods and microorganisms are provided, where novel genetic mammalian cell invasive capability is imparted to a microorganism by the introduction of an exogenous inv or ail gene. The resulting organisms are then capable of binding to mammalian cells and are transferred to the cytoplasm. Other novel genetic capabilities may be imparted to the unicellular microorganism, which may serve as a vaccine for one or more pathogens or may introduce genetic capabilities or foreign molecules into a mammalian host cell. The sequences may be used for an in vitro screen for pathogenicity.

Abstract: Nucleic acids encoding all or part of a Yersinia ail gene are provided. The nucleic acid comprises at least 50 base pairs of a Yersinia ail gene in isolated form or consists or a fragment consisting essentially of at least 50 base pairs but not more than 50 kilo base pairs of a Yersinia ail gene. Such nucleic acids can also be operably linked to transcriptional and translational initiation and termination sequences which are functional in a microorganism host.

Abstract: A vaccine effective in protecting mammals against urinary infections is prepared from purified Gal-Gal pilus proteins or fragments thereof.

Abstract: Method and compositions for infectious disease diagnosis and epidemiology involving labeled nucleotide probes complementary to nucleic acid coding for a characteristic pathogen product. Clinical isolates are cultivated, expanding the number of microorganisms, the resulting colonies lysed, the genome normally denatured and then fixed. Alternatively, clinical samples (stool, sputum, pus, etc.) are spotted onto an inert support. The sample is treated in such a way that the DNA is liberated from microbes present in the sample and complexed onto the support. The DNA is normally denatured and fixed in this process. Subsequently, a labelled polynucleotide probe specific for a DNA sequence characteristic of a pathogenic product suspected of being present in the clinical sample is contacted with the fixed genomic single stranded nucleic acid under hybridizing conditions. Hybridization of probes to the single stranded nucleic acid is diagnostic of the presence of the pathogen.