Patents by Inventor Stanley Fields
Stanley Fields has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200363423Abstract: This disclosure provides a non-genetic, high-throughput method of introducing and assaying effects of amino acid substitutions at a plurality of positions in a target protein. Mistranslation events are imposed stochastically during the translation of multiple copies of a target protein, resulting in a plurality of variants of the target protein (i.e., protein quasi-species). The analysis can be performed simultaneously for a plurality of different target proteins, including the entire proteome of a cell to produce a statistical proteome. The plurality of quasi-species or statistical proteome can be subjected to functional assays, such as enrichment or profiling assays, to determine the functional effect of the substitutions on the target protein(s).Type: ApplicationFiled: November 9, 2019Publication date: November 19, 2020Applicant: University of WashingtonInventors: Judit Villen, Ricard Rodriguez-Mias, Stanley Fields
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Publication number: 20170363648Abstract: Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.Type: ApplicationFiled: August 31, 2017Publication date: December 21, 2017Inventors: George M. Church, Justin Feng, Daniel J. Mandell, David Baker, Stanley Fields, Benjamin Ward Jester, Christine Elaine Tinberg
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Patent number: 9766255Abstract: Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.Type: GrantFiled: January 12, 2016Date of Patent: September 19, 2017Assignees: President and Fellows of Harvard College, University of WashingtonInventors: George M. Church, Justin Feng, Daniel J. Mandell, David Baker, Stanley Fields, Benjamin Ward Jester, Christine Elaine Tinberg
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Publication number: 20170198363Abstract: Compositions and methods are provided for the detection of small molecules in a cell using biosensor molecules comprising conditionally active ligand binding domains. Compositions for conditionally activating transcription based on the presence of a small molecules in a cell are further provided, together with methods of designing, producing, and expressing biosensor molecules in cells.Type: ApplicationFiled: December 28, 2016Publication date: July 13, 2017Inventors: JUNE MEDFORD, MAURICIO ANTUNES, KEVIN MOREY, BENJAMIN JESTER, CHRISTINE TINBERG, STANLEY FIELDS, DAVID BAKER, MATTHEW BICK
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Publication number: 20160202256Abstract: Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.Type: ApplicationFiled: January 12, 2016Publication date: July 14, 2016Inventors: George M. Church, Justin Feng, Daniel J. Mandell, David Baker, Stanley Fields, Benjamin Ward Jester, Christine Elaine Tinberg
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Publication number: 20160017410Abstract: Disclosed is a method for multiplexed mutagenesis of a target nucleotide sequence. The method entails generating, in parallel, a set of mutagenic oligonucleotide primers designed to cover all or part of the target nucleotide sequence, and reacting the set of mutagenic oligonucleotide primers with the target sequence in the presence of a polymerase to generate a mutant nucleotide sequence library, wherein each member of the mutant nucleotide sequence library comprises a full-length copy of the target nucleotide sequence having a unique programmed mutation derived from one member of the set of mutagenic oligonucleotide primers. Also disclosed are methods for generating a mutant nucleotide sequence library and for generating a mutant protein library.Type: ApplicationFiled: July 17, 2015Publication date: January 21, 2016Inventors: Jay Shendure, Stanley Fields, Jacob Kitzman, Lea Starita
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Publication number: 20100280060Abstract: Identification of nucleic acids involved in life span diseases and disorders or related diseases and disorders, and the use of such methods for identifying candidate agents which modulate life span diseases and disorders or related diseases and disorders are provided. Compositions and methods for treating life span diseases and disorders or related diseases and disorders are provided.Type: ApplicationFiled: December 12, 2007Publication date: November 4, 2010Applicant: The University of WashingtonInventors: Ralph W. Powers, III, Matthew R. Kaeberlein, Stanley Fields
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Publication number: 20060234250Abstract: Identification of nucleic acids involved in life span diseases and disorders or related diseases and disorders, and the use of such methods for identifying candidate agents which modulate life span diseases and disorders or related diseases and disorders are provided. Compositions and methods for treating life span diseases and disorders or related diseases and disorders are provided.Type: ApplicationFiled: April 15, 2005Publication date: October 19, 2006Inventors: Ralph Powers, Matthew Kaeberlein, Stanley Fields
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Patent number: 5750667Abstract: A method for detecting an interaction between an RNA-binding protein and a test RNA molecule is disclosed. This method comprises providing a host cell containing a detectable gene. The detectable gene expresses a detectable protein when the detectable gene is activated by an amino acid sequence including a transcriptional activation domain when the transcriptional activation domain is in sufficient proximity to the detectable gene. First, second and third chimeric genes are also provided. The first chimeric gene comprises a DNA-binding domain that recognizes a binding site on the detectable gene in the host cell and a first RNA-binding domain. The second chimeric gene comprises a transcriptional activation domain and a second RNA-binding domain. The third chimeric gene comprises a first RNA sequence capable of binding to either the first or second RNA-binding and a second RNA sequence to be tested for interaction with the RNA-binding protein not bound to the first RNA sequence.Type: GrantFiled: November 12, 1996Date of Patent: May 12, 1998Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Stanley Fields
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Patent number: 5677131Abstract: A method for detecting an interaction between an RNA-binding protein and a test RNA molecule is disclosed. This method comprises providing a host cell containing a detectable gene. The detectable gene expresses a detectable protein when the detectable gene is activated by an amino acid sequence including a transcriptional activation domain when the transcriptional activation domain is in sufficient proximity to the detectable gene. First, second and third chimeric genes are also provided. The first chimeric gene comprises a DNA-binding domain that recognizes a binding site on the detectable gene in the host cell and a first RNA-binding domain. The second chimeric gene comprises a transcriptional activation domain and a second RNA-binding domain. The third chimeric gene comprises a first RNA sequence capable of binding to either the first or second RNA-binding and a second RNA sequence to be tested for interaction with the RNA-binding protein not bound to the first RNA sequence.Type: GrantFiled: July 9, 1996Date of Patent: October 14, 1997Assignee: Wisconsin Alumni Research FoundationInventors: Marvin P. Wickens, Stanley Fields
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Patent number: 5667973Abstract: A method is provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.Type: GrantFiled: June 7, 1995Date of Patent: September 16, 1997Assignee: The Research Foundation of State University of New YorkInventors: Stanley Fields, Ok-Kyu Song
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Patent number: 5610015Abstract: A method for detecting an interaction between an RNA-binding protein and a test RNA molecule is disclosed. This method comprises providing a host cell containing a detectable gene. The detectable gene expresses a detectable protein when the detectable gene is activated by an amino acid sequence including a transcriptional activation domain when the transcriptional activation domain is in sufficient proximity to the detectable gene. First, second and third chimeric genes are also provided. The first chimeric gene comprises a DNA-binding domain that recognizes a binding site on the detectable gene in the host cell and a first RNA-binding domain. The second chimeric gene comprises a transcriptional activation domain and a second RNA-binding domain. The third chimeric gene comprises a first RNA sequence capable of binding to either the first or second RNA-binding and a second RNA sequence to be tested for interaction with the RNA-binding protein not bound to the first RNA sequence.Type: GrantFiled: March 23, 1995Date of Patent: March 11, 1997Assignees: Wisconsin Alumni Research Foundation, State University of New York at Stony BrookInventors: Marvin P. Wickens, Stanley Fields
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Patent number: 5468614Abstract: A method and kit are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.Type: GrantFiled: February 1, 1994Date of Patent: November 21, 1995Assignee: The Research Foundation of State University of New YorkInventors: Stanley Fields, Ok-Kyu Song
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Patent number: 5283173Abstract: Methods are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.Type: GrantFiled: January 24, 1990Date of Patent: February 1, 1994Assignee: The Research Foundation of State University of New YorkInventors: Stanley Fields, Ok-Kyu Song