Patents by Inventor Stanley N. Cohen

Stanley N. Cohen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 4440853
    Abstract: Methods for microbiological processing of organic materials for production of valuable products. Asymmetric hollow fibers are employed in a flow reactor, where the hollow fibers have a semipermeable membrane surrounding a lumen, where the semipermeable membrane is supported by a sponge structure. The pores of the sponge structure serve as a housing for microorganisms or cells with high density packing of the microorganisms or cells in the pores. Nutrient medium continuously flowing through the lumen provides nutrients to the microorganisms or cells as well as any substrates to be processed by the microorganisms or cells. The nutrients and substrates diffuse through the semipermeable membrane into the pores, where they are processed, and the metabolic products diffuse into the lumen. The lumen effluent is then processed for the desired products. Optionally, oxygen is provided external to the hollow fiber to enhance the amount of oxygen available to the microorganisms and cells.
    Type: Grant
    Filed: June 20, 1983
    Date of Patent: April 3, 1984
    Assignee: Board of Trustees of the Leland Stanford Junior University
    Inventors: Alan S. Michaels, Channing R. Robertson, Stanley N. Cohen
  • Patent number: 4374927
    Abstract: Microbiological methods, compositions and transformants are used for production of organic products for controlling cellular properties. Extrachromosomal elements are used which are subject to external modulation for production of a control element. The change in the amount of production of the control element allows for enhanced expression of a gene producing a poly(amino acid) product. The change in production of the control element allowing for enhanced gene expression of the product is accompanied by amplification of the product producing gene.
    Type: Grant
    Filed: February 24, 1981
    Date of Patent: February 22, 1983
    Assignee: The Board of Trustees of the Leland Stanford Jr. University
    Inventors: John J. Sninsky, Stanley N. Cohen
  • Patent number: 4293652
    Abstract: A method and a DNA linker are described for synthesizing relatively long double-stranded deoxyribonucleic acid sequences of defined composition. Short complementary single strand segments of oligonucleotides comprising part of the full sequence desired are synthesized using known procedures. Overlapping single strand segments are annealed forming double-stranded fragments which are inserted in cloning vectors and cloned in an appropriate host, both purifying the DNA fragments and amplifying the amount thereof. An adjacent fragment is then similarly synthesized in quantity and such fragments are inserted adjacent to the first synthetic introduced fragments in the cloning vectors, followed by cloning in an appropriate host. The procedure continues until the entire desired sequence has been formed, at which time it may be excised or cloned directly in the vectors upon which it was made.
    Type: Grant
    Filed: May 25, 1979
    Date of Patent: October 6, 1981
    Assignee: Cetus Corporation
    Inventor: Stanley N. Cohen
  • Patent number: 4237224
    Abstract: Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini to which is inserted a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids and proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like.
    Type: Grant
    Filed: January 4, 1979
    Date of Patent: December 2, 1980
    Assignee: Board of Trustees of the Leland Stanford Jr. University
    Inventors: Stanley N. Cohen, Herbert W. Boyer