Abstract: Disclosed are methods and systems to detect a class of non-variola Orthopoxviruses, and, more particularly, monkeypox virus. In certain embodiments, the method may comprise obtaining a sample from the subject; and detecting a nucleic acid sequence specific to the non-variola Orthopoxvirus. In some embodiments, the method may further isolation of the non-variola Orthopoxvirus from a solid substrate such as a tissue swab and further purification of viral nucleic acid using silica beads. The sample may in some cases be self-collected by a subject. The disclosed methods and systems, including kits for self-collection, may be used for a high throughput assay allowing for screening of hundreds of samples per day.

Abstract: A switch system for an industrial machine is described which combines both optical and capacitance switches in a switch assembly. A photo-optical switch is positioned in each assembly to provide an output signal upon the introduction of a human hand into an opening formed therein, and a capacitance sensitive touch plate switch is positioned within the opening to provide an output signal upon contact by a human hand. Circuitry responds to the substantially simultaneous activation of both the optical and capacitance switches within the switch assembly and produces an output signal. Typically two of such switch assemblies are used to control power to an industrial machine.