Patents by Inventor Steen Troels Jørgensen
Steen Troels Jørgensen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220298517Abstract: The present invention relates to a method for counter-selection by inhibition of conditionally essential genes.Type: ApplicationFiled: June 16, 2020Publication date: September 22, 2022Applicant: Novozymes A/SInventors: Steen Troels Joergensen, Michael Dolberg Rasmussen
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Publication number: 20200196617Abstract: A method for improving the extensibility of a dough comprising a) adding a gamma glutamyl transpeptidase to flour or to a dough comprising a flour; and b) making the dough. The gamma glutamyl transpeptidase may be obtainable from Bacillus licheniformis and from Bacillus horikoshii.Type: ApplicationFiled: June 20, 2018Publication date: June 25, 2020Applicant: Novozymes A/SInventors: Lisbeth Kalum, Sara Maria Landvik, Irina Victorovna Matveeva, Steen Troels Joergensen, Kenneth Jensen
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Publication number: 20190276855Abstract: The present invention relates to methods for the site-specific integration of at least one polynucleotide of interest into the chromosome of a Bacillus licheniformis host cell using the FLP/FRT system derived from Saccharomoces cerevisiae or a homologue or variant thereof.Type: ApplicationFiled: October 23, 2017Publication date: September 12, 2019Applicant: Novozymes A/SInventor: Steen Troels Joergensen
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Patent number: 9803209Abstract: Provided herein are Lactobacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous gene encoding a restriction modification system protein. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.Type: GrantFiled: May 17, 2013Date of Patent: October 31, 2017Assignee: Novozymes A/SInventors: Steen Troels Joergensen, Torsten Bak Regueira, Brian Kobmann, Peter Bjarke Olsen, Bjarke Christensen
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Patent number: 9428558Abstract: The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase from Bacillus licheniformis and of alpha amylase from Bacillus amyloliquefaciens.Type: GrantFiled: March 22, 2012Date of Patent: August 30, 2016Assignee: Novozymes A/SInventors: Steen Troels Joergensen, Anne Breüner
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Patent number: 9334493Abstract: The invention relates to a method of indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level from a library of polynucleotides encoding the same polypeptide of interest, or variants or homologues thereof, in a host cell expression library, the method comprising the steps of: a) providing an expression library in a host cell, said library comprising at least two different open reading frames encoding the same polypeptide of interest, or variants or homologues thereof, each open reading frame being translationally coupled to a downstream gene encoding a screenable or selectable reporter; b) culturing the host cell under conditions conducive to the expression of the polypeptide of interest, or variant or homologue thereof; and c) screening and/or selecting for a host cell having an improved expression level of the translationally coupled reporter when compared to one or more other host cells of the library, thus indirectly identifying a polynucleotide comprisiType: GrantFiled: December 20, 2007Date of Patent: May 10, 2016Assignee: Novozymes A/SInventor: Steen Troels Joergensen
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Publication number: 20150147783Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: February 9, 2015Publication date: May 28, 2015Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
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Publication number: 20150125959Abstract: Provided herein are Lactobacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous gene encoding a restriction modification system protein. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.Type: ApplicationFiled: May 17, 2013Publication date: May 7, 2015Inventors: Steen Troels Joergensen, Torsten Bak Regueira, Brian Kobmann, Peter Bjarke Olsen, Bjarke Christensen
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Patent number: 8728804Abstract: The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.Type: GrantFiled: October 28, 2011Date of Patent: May 20, 2014Assignee: Novozymes A/SInventors: Bjarke Christensen, Peter Bjarke Olsen, Torsten Bak Regueira, Steen Troels Joergensen, Alan Berry
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Publication number: 20140073009Abstract: The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase from Bacillus licheniformis and of alpha amylase from Bacillus amyloliquefaciens.Type: ApplicationFiled: March 22, 2012Publication date: March 13, 2014Applicant: Novozymes A/SInventors: Steen Troels Joergensen, Anne Breüner
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Publication number: 20130295644Abstract: The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.Type: ApplicationFiled: October 28, 2011Publication date: November 7, 2013Inventors: Bjarke Christensen, Peter Bjarke Olsen, Torsten Bak Regueira, Steen Troels Joergensen, Alan Berry
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Publication number: 20130280775Abstract: The present invention relates to methods of producing n-propanol, isopropanol, and coproducing n-propanol with isopropanol. The present invention also relates to methods for producing propylene, as well as host cells capable of n-propanol and isopropanol production.Type: ApplicationFiled: October 28, 2011Publication date: October 24, 2013Applicants: Novozymes, Inc., Novozymes A/SInventors: Thomas Grotkjaer, Bjarke Christensen, Torsten Bak Regueira, Steen Troels Joergensen, Alan Berry
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Publication number: 20090317865Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: August 21, 2009Publication date: December 24, 2009Applicant: Novozymes A/SInventors: Steen Troels Jørgensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
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Patent number: 6808896Abstract: The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a bacterial host strain chromosome, which already comprises at least one copy of the gene of interest in a different position.Type: GrantFiled: August 13, 2001Date of Patent: October 26, 2004Assignee: Novozymes A/SInventors: Steen Troels Jørgensen, Jens Toenne Andersen, Michael Dolbjerg Rasmussen, Carsten Olsen
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Patent number: 6428981Abstract: A Bacillus cell for improved production of a polypeptide of interest and a process for producing a polypeptide of interest are disclosed.Type: GrantFiled: April 4, 2000Date of Patent: August 6, 2002Assignee: Novozymes A/SInventors: Steen Troels Joergensen, Christina Lund Christensen, Tina Kristensen
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Patent number: 6280721Abstract: The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.Type: GrantFiled: January 25, 1995Date of Patent: August 28, 2001Assignee: Valent BioSciences, Inc.Inventors: Lee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner, Steen Troels Jørgensen, Per Linå Jørgensen, Børge Krag Diderichsen
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Patent number: 6270760Abstract: The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.Type: GrantFiled: June 10, 1997Date of Patent: August 7, 2001Assignee: Valent BioSciences, Inc.Inventors: Lee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner, Steen Troels Jørgensen, Per Linå Jørgensen, Børge Krag Diderichsen