Patents by Inventor Steen Troels Jorgensen
Steen Troels Jorgensen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10640757Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: GrantFiled: September 23, 2013Date of Patent: May 5, 2020Assignee: Novozymes A/SInventors: Steen Troels Jorgensen, Poul Erik Pedersen
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Patent number: 8993304Abstract: The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.Type: GrantFiled: April 2, 2013Date of Patent: March 31, 2015Assignee: Novozymes A/SInventor: Steen Troels Jorgensen
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Patent number: 8911969Abstract: A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.Type: GrantFiled: March 5, 2009Date of Patent: December 16, 2014Inventors: Jens Tonne Andersen, Steen Troels Jorgensen, Michael Dolbjerg Rasmussen, Peter Bjarke Olsen, Ib Groth Clausen
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Publication number: 20140106457Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: ApplicationFiled: September 23, 2013Publication date: April 17, 2014Inventors: Steen Troels Jorgensen, Poul Erik Pedersen
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Patent number: 8431382Abstract: The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.Type: GrantFiled: May 29, 2007Date of Patent: April 30, 2013Assignee: Novozymes A/SInventor: Steen Troels Jorgensen
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Publication number: 20100120623Abstract: The invention relates to a method of indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level from a library of polynucleotides encoding the same polypeptide of interest, or variants or homologues thereof, in a host cell expression library, the method comprising the steps of: a) providing an expression library in a host cell, said library comprising at least two different open reading frames encoding the same polypeptide of interest, or variants or homologues thereof, each open reading frame being translationally coupled to a downstream gene encoding a screenable or selectable reporter; b) culturing the host cell under conditions conducive to the expression of the polypeptide of interest, or variant or homologue thereof; and c) screening and/or selecting for a host cell having an improved expression level of the translationally coupled reporter when compared to one or more other host cells of the library, thus indirectly identifying a polynucleotide comprisiType: ApplicationFiled: December 20, 2007Publication date: May 13, 2010Applicant: Novozymes A/SInventor: Steen Troels Jorgensen
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Publication number: 20090317865Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: August 21, 2009Publication date: December 24, 2009Applicant: Novozymes A/SInventors: Steen Troels Jørgensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
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Publication number: 20090280569Abstract: The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.Type: ApplicationFiled: May 29, 2007Publication date: November 12, 2009Applicant: Novozymes A/SInventor: Steen Troels Jorgensen
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Publication number: 20090203074Abstract: A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.Type: ApplicationFiled: April 2, 2009Publication date: August 13, 2009Applicant: Novozymes A/SInventors: Jens Tonne Andersen, Steen Troels Jorgensen, Michael Dolbjerg Rasmussen, Peter Bjarke Olsen, Ib Groth Clausen
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Publication number: 20090176290Abstract: A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.Type: ApplicationFiled: March 5, 2009Publication date: July 9, 2009Applicant: Novozymes A/SInventors: Jens Tonne Andersen, Steen Troels Jorgensen, Michael Dolbjerg Rasmussen, Peter Bjarke Olsen, Ib Groth Clausen
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Patent number: 7521204Abstract: A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.Type: GrantFiled: March 25, 2003Date of Patent: April 21, 2009Assignee: Novozymes A/SInventors: Jens Tonne Andersen, Steen Troels Jorgensen, Michael Dolbjerg Rasmussen, Peter Bjarke Olsen, Ib Groth Clausen
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Patent number: 7157262Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.Type: GrantFiled: August 30, 2002Date of Patent: January 2, 2007Assignee: Novozymes A/SInventors: Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
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Patent number: 6808896Abstract: The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a bacterial host strain chromosome, which already comprises at least one copy of the gene of interest in a different position.Type: GrantFiled: August 13, 2001Date of Patent: October 26, 2004Assignee: Novozymes A/SInventors: Steen Troels Jørgensen, Jens Toenne Andersen, Michael Dolbjerg Rasmussen, Carsten Olsen
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Publication number: 20030199069Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.Type: ApplicationFiled: August 30, 2002Publication date: October 23, 2003Applicant: Novozymes A/SInventors: Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
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Publication number: 20030032186Abstract: The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a bacterial host strain chromosome, which already comprises at least one copy of the gene of interest in a different position.Type: ApplicationFiled: August 13, 2001Publication date: February 13, 2003Applicant: Novozymes A/SInventors: Steen Troels Jorgensen, Jens Toenne Andersen, Michael Dolbjerg Rasmussen, Carsten Olsen
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Patent number: 6495357Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.Type: GrantFiled: January 14, 1998Date of Patent: December 17, 2002Assignee: Novozyme A/SInventors: Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
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Patent number: 6280721Abstract: The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.Type: GrantFiled: January 25, 1995Date of Patent: August 28, 2001Assignee: Valent BioSciences, Inc.Inventors: Lee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner, Steen Troels Jørgensen, Per Linå Jørgensen, Børge Krag Diderichsen
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Patent number: 6270760Abstract: The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.Type: GrantFiled: June 10, 1997Date of Patent: August 7, 2001Assignee: Valent BioSciences, Inc.Inventors: Lee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner, Steen Troels Jørgensen, Per Linå Jørgensen, Børge Krag Diderichsen
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Patent number: 6066473Abstract: The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.Type: GrantFiled: July 22, 1998Date of Patent: May 23, 2000Assignee: Novo Nordisk A/SInventors: Martin Tangney, Christian Hansen, Poul Erik Pedersen, Per Lina Jorgensen, Steen Troels Jorgensen
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Patent number: 5843720Abstract: The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.Type: GrantFiled: September 8, 1997Date of Patent: December 1, 1998Assignee: Novo Nordisk A/SInventors: Martin Tangney, Christian Hansen, Poul Erik Pedersen, Per Lina Jorgensen, Steen Troels Jorgensen