Abstract: The invention concerns an in vitro method for producing a 3D structure of endoderm cells, mesoderm cells and ectoderm cells and capable of further organogenesis to form an organ or organ-like cell structure. The method comprises seeding one or more pluripotent stem cells in a culture chamber containing culture medium, followed by culturing the seeded cell or cells in culture medium comprising an amount of chemical inducers for forming a plurality of pluripotent stem cells and inducing part of the formed pluripotent stem cells to form extraembryonic stem cells to obtain a primitive endoderm/epiblast aggregate. The aggregate is cultured in fresh culture medium to obtain an extraembryonic endoderm/epiblast rosette structure. Culture medium is flushed from the extraembryonic endoderm/epiblast rosette structure and the extraembryonic endoderm/epiblast rosette structure is cultured in fresh serum free culture medium comprising a reducing agent to obtain the 3D structure of cells.

Abstract: A vascular model includes at least one microchannel perfusable by at least one fluid and in a form of a hollow structure having a wall thickness in a range from 0.1 ?m to 1000 ?m, a concave inner surface with a cross-section which is circular in part and at least one pore having a diameter in a range from 1 nm to 100 ?m. The at least one microchannel has a width in a range from 0.01 ?m to 10 mm and a depth in a range from 0.01 ?m to 10 mm. At least one chamber is perfusable by a same and/or different fluid and surrounds the at least one microchannel over an entire length and width or over parts thereof. The at least one chamber adjoins the at least one microchannel. At least one connector is configured to receive and/or discharge the at least one fluid.

Abstract: The present invention relates to a three-dimensional (3D) model of a hematopoietic stem and progenitor cell (HSPC) niche, that comprises the coculture of human HSPC and human mesenchymal stromal cells in a defined 3D environment and thereby procures vital stem cell functions.

Abstract: A vascular model includes at least one microchannel perfusable by at least one fluid and in a form of a hollow structure having a wall thickness in a range from 0.1 ?m to 1000 ?m, a concave inner surface with a cross-section which is circular in part and at least one pore having a diameter in a range from 1 nm to 100 ?m. The at least one microchannel has a width in a range from 0.01 ?m to 10 mm and a depth in a range from 0.01 ?m to 10 mm. At least one chamber is perfusable by a same and/or different fluid and surrounds the at least one microchannel over an entire length and width or over parts thereof. The at least one chamber adjoins the at least one microchannel. At least one connector is configured to receive and/or discharge the at least one fluid.

Abstract: A method for producing a molded body, said method comprising: providing a film comprising a thermoplastic plastic and having a film thickness D ranging from 1 ?m to 1000 ?m; irradiating the film with ionizing radiation, to produce irradiated regions in the film; thermally reshaping the film into a molded body and generating at least one hollow structure, wherein a temperature of the thermal reshaping remains below the melting temperature for the thermoplastic plastic; removing the irradiated regions, to create pores having a diameter ? from about 10 nm to about 10 ?m in the molded body; and removing the molded body from a mold.

Abstract: A method for producing a layer on a molded article. The method includes providing a formable film. Galvanically catalytically active nuclei are anchored to at least one region of the formable film provided for the layer. The formable film is shaped so as to form the molded article. A galvanic deposition is performed on a surface of the molded article so as to bond the nuclei to form the layer.

Abstract: A method for producing a molded body, said method comprising: providing a film comprising a thermoplastic plastic and having a film thickness D ranging from 1 ?m to 1000 ?m; irradiating the film with ionizing radiation, to produce irradiated regions in the film; thermally reshaping the film into a molded body and generating at least one hollow structure, wherein a temperature of the thermal reshaping remains below the melting temperature for the thermoplastic plastic; removing the irradiated regions, to create pores having a diameter ? from about 10 nm to about 10 ?m in the molded body; and removing the molded body from a mold.

Abstract: The invention relates to a bioreactor including a housing having a lower housing half and an upper housing half that are respectively connected to one another on one side by a film joint. At least one of the upper or lower housing halves includes a device to exchange fluids. The bioreactor further includes at least one support element to support at least one cell culture substrate. The invention furthermore relates to an arrangement of such bioreactors, processes for production thereof and use thereof for culturing cells or as an implant.

Abstract: A method for producing a layer on a molded article. The method includes providing a formable film. Galvanically catalytically active nuclei are anchored to at least one region of the formable film provided for the layer. The formable film is shaped so as to form the molded article. A galvanic deposition is performed on a surface of the molded article so as to bond the nuclei to form the layer.

Abstract: A molded body comprises a film with a film thickness D ranging from 1 ?m to 1000 ?m, with at least one hollow structure configured into the film. Each hollow structure has an outside diameter d equal to at least double value of film thickness D, a height h is equal to or less than double the value of the outside diameter d, a wall thickness b greater than 0.02 times the film thickness D and less than or equal to the film thickness D and a local curvature radius r greater than 0.2 times and less than or equal to 5 times the wall thickness b. The film and the hollow structure include a plurality of pores.

Abstract: Method of playing roulette with a system including a roulette wheel, a conventional betting layout and a progressive jackpot layout. Players make wages on the conventional layout and/or the progressive layout to participate in a progressive jackpot game wherein the jackpot is accumulated from losing wagers on the progressive jackpot layout. The system includes sensors for detecting chips placed on the progressive layout. The jackpot is won by a wagered winning number being selected by the roulette wheel in a predetermined number of successive spins.