Patents by Inventor Stefan Howorka

Stefan Howorka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210301334
    Abstract: A membrane-spanning nanopore is provided, the nanopore comprising: a. one or more polynucleotide strands that provide a scaffold coma) ponent; and b. a plurality of polynucleotide strands that provide a plurality of staple components; wherein each of the plurality of staple components hybridise to the scaffold component; wherein the orientation of a major portion 7 nm of at least one polynucleotide strand comprised within the scaffold component is substantially parallel to a planar surface of a membrane as well as embedded within and substantially coplanar with the membrane; wherein the nanopore defines a channel that is suitable for perforating the membrane; the channel b) having a longitudinal axis extending along the centre thereof and a minimum internal dimension perpendicular to the longitudinal axis of at least about 3 nm.
    Type: Application
    Filed: August 2, 2019
    Publication date: September 30, 2021
    Applicant: UCL Business Ltd
    Inventors: Stefan HOWORKA, Yongzheng XING
  • Publication number: 20210292376
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: September 23, 2021
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Patent number: 11034734
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Grant
    Filed: July 25, 2019
    Date of Patent: June 15, 2021
    Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20200017556
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: July 25, 2019
    Publication date: January 16, 2020
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20190309350
    Abstract: A membrane-spanning nanopore is provided that comprises: i. at least one scaffold polynucleotide strand; ii. a plurality of staple polynucleotide strands; and iii. at least one hydrophobically-modified polynucleotide strand, wherein the at least one hydropho-bically-modified polynucleotide strand comprises a polynucleotide strand and a hydrophobic moiety; wherein each of the plurality of staple polynucleotide strands hybridises to the at least one scaffold polynucleotide strand to form the three-dimensional structure of the membrane-spanning nanopore, and wherein the at least one hydrophobically-modified polynucleotide strand hybridises to a portion of the at least one scaffold polynucleotide strand, the membrane-spanning nanopore defining a central channel with a minimum internal width of at least about 5 nm. Membranes comprising the membrane-spanning nanopore and applications of those membranes are also provided.
    Type: Application
    Filed: July 14, 2017
    Publication date: October 10, 2019
    Applicant: UCL BUSINESS PLC
    Inventors: Stefan HOWORKA, Genevieve PUGH, Jonathan Richard BURNS
  • Patent number: 10400014
    Abstract: The invention relates to mutant forms of the outer membrane-located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: September 3, 2019
    Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20180148481
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: September 1, 2015
    Publication date: May 31, 2018
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20170283470
    Abstract: The invention relates to mutant forms of the outer membrane located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.
    Type: Application
    Filed: September 1, 2015
    Publication date: October 5, 2017
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20070087382
    Abstract: The invention relates to assemblies for bonding molecules comprising bondable functional groups, which are present on a solid supporting material as individual molecular functional groups or multiple identical functional groups. Said assemblies are characterised in that the density of the individual functional groups or multiple functional groups on the solid supporting material is between 104 and 1010 individual or multiple functional groups per cm2 and that there are no additional bondable functional groups within a selected distance d from any individual bondable functional group or multiple functional group for at least 95% and in particular at least 99% of the individual or multiple functional groups.
    Type: Application
    Filed: September 10, 2004
    Publication date: April 19, 2007
    Applicant: Upper Austrian Research GmbH
    Inventors: Stefan Howorka, Patrick Pammer
  • Publication number: 20050208574
    Abstract: Provided are pore-subunit polypeptides covalently linked to one or more sensing moieties, and uses of these modified polypeptides to detect and/or measure analytes or physical characteristics within a given sample.
    Type: Application
    Filed: May 16, 2005
    Publication date: September 22, 2005
    Inventors: Hagan Bayley, Stefan Howorka, Liviu Movileanu
  • Publication number: 20040265936
    Abstract: The invention concerns a process for the recombinant production of S-layer proteins in gram-negative host cells. Furthermore the nucleotide sequence of a new S-layer gene and processes for the production of modified S-layer proteins are disclosed.
    Type: Application
    Filed: July 14, 2004
    Publication date: December 30, 2004
    Applicants: NANO-S Biotechnologie GmbH
    Inventors: Werner Lubitz, Uwe Sleytr, Beatrix Kuen, Michaela Truppe, Stefan Howorka, Stepanka Resch, Gerhard Schroll, Margit Sara
  • Patent number: 6777202
    Abstract: The invention concerns a process for the recombinant production of S-layer proteins in gram-negative host cells. Furthermore the nucleotide sequence of a new S-layer gene and processes for the production of modified S-layer proteins are disclosed.
    Type: Grant
    Filed: December 2, 1998
    Date of Patent: August 17, 2004
    Assignees: NANO-S Biotechnologie GmbH
    Inventors: Werner Lubitz, Uwe Sleytr, Beatrix Kuen, Michaela Truppe, Stefan Howorka, Stepanka Resch, Gerhard Schroll, Margit Sara
  • Publication number: 20020168728
    Abstract: The invention concerns a process for the recombinant production of S-layer proteins in gram-negative host cells. Furthermore the nucleotide sequence of a new S-layer gene and processes for the production of modified S-layer proteins are disclosed.
    Type: Application
    Filed: December 2, 1998
    Publication date: November 14, 2002
    Applicant: ARENT FOX KINTNER PLOTKIN AND KAHN, PLLC
    Inventors: WERNER LUBITZ, UWE SLEYTR, BEATRIX KUEN, MICHAELA TRUPPE, STEFAN HOWORKA, STEPANKA RESCH, GERHARD SCHROLL, MARGIT SARA