Patents by Inventor Stefan Howorka
Stefan Howorka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230392169Abstract: Improved nucleic acid nanostructures provide a platform for stable and effective intra-cellular delivery of nucleic acids, suitably coding nucleic acids such as mRNA or ssDNA. A nucleic acid nanostructure is provided that comprises a first single stranded nucleic acid sequence that defines a scaffold sequence, wherein the scaffold sequence comprises at least one open reading frame that encodes a first gene product; and a plurality of single stranded nucleic acid sequences that define a plurality of staple sequences, wherein the plurality of staple sequences are capable of hybridising with one or more regions of the scaffold sequence in order to induce the formation of a geometrically defined higher order structure. The nanostructure may further comprise at least one membrane binding moiety, wherein the membrane binding moiety is configured to associate with a cell membrane.Type: ApplicationFiled: September 23, 2021Publication date: December 7, 2023Inventors: Stefan HOWORKA, Jonathan BURNS, Matthew REEVES, Nishkantha ARULKUMARAN, Mervyn SINGER
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Publication number: 20230120851Abstract: A membrane-spanning nanopore is provided that comprises: i. at least one scaffold polynucleotide strand; ii. a plurality of staple polynucleotide strands; and iii. at least one hydrophobically-modified polynucleotide strand, wherein the at least one hydrophobically-modified polynucleotide strand comprises a polynucleotide strand and a hydrophobic moiety; wherein each of the plurality of staple polynucleotide strands hybridises to the at least one scaffold polynucleotide strand to form the three-dimensional structure of the membrane-spanning nanopore, and wherein the at least one hydrophobically-modified polynucleotide strand hybridises to a portion of the at least one scaffold polynucleotide strand, the membrane-spanning nanopore defining a central channel with a minimum internal width of at least about 5 nm.Type: ApplicationFiled: September 9, 2022Publication date: April 20, 2023Applicant: UCL BUSINESS LTDInventors: Stefan HOWORKA, Genevieve PUGH, Jonathan Richard BURNS
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Patent number: 11485995Abstract: A membrane-spanning nanopore is provided that comprises: i. at least one scaffold polynucleotide strand; ii. a plurality of staple polynucleotide strands; and iii. at least one hydrophobically-modified polynucleotide strand, wherein the at least one hydrophobically-modified polynucleotide strand comprises a polynucleotide strand and a hydrophobic moiety; wherein each of the plurality of staple polynucleotide strands hybridises to the at least one scaffold polynucleotide strand to form the three-dimensional structure of the membrane-spanning nanopore, and wherein the at least one hydrophobically-modified polynucleotide strand hybridises to a portion of the at least one scaffold polynucleotide strand, the membrane-spanning nanopore defining a central channel with a minimum internal width of at least about 5 nm. Membranes comprising the membrane-spanning nanopore and applications of those membranes are also provided.Type: GrantFiled: July 14, 2017Date of Patent: November 1, 2022Assignee: UCL BUSINESS LTDInventors: Stefan Howorka, Genevieve Pugh, Jonathan Richard Burns
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Publication number: 20210301334Abstract: A membrane-spanning nanopore is provided, the nanopore comprising: a. one or more polynucleotide strands that provide a scaffold coma) ponent; and b. a plurality of polynucleotide strands that provide a plurality of staple components; wherein each of the plurality of staple components hybridise to the scaffold component; wherein the orientation of a major portion 7 nm of at least one polynucleotide strand comprised within the scaffold component is substantially parallel to a planar surface of a membrane as well as embedded within and substantially coplanar with the membrane; wherein the nanopore defines a channel that is suitable for perforating the membrane; the channel b) having a longitudinal axis extending along the centre thereof and a minimum internal dimension perpendicular to the longitudinal axis of at least about 3 nm.Type: ApplicationFiled: August 2, 2019Publication date: September 30, 2021Applicant: UCL Business LtdInventors: Stefan HOWORKA, Yongzheng XING
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Publication number: 20210292376Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.Type: ApplicationFiled: March 8, 2021Publication date: September 23, 2021Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Patent number: 11034734Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.Type: GrantFiled: July 25, 2019Date of Patent: June 15, 2021Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20200017556Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.Type: ApplicationFiled: July 25, 2019Publication date: January 16, 2020Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20190309350Abstract: A membrane-spanning nanopore is provided that comprises: i. at least one scaffold polynucleotide strand; ii. a plurality of staple polynucleotide strands; and iii. at least one hydrophobically-modified polynucleotide strand, wherein the at least one hydropho-bically-modified polynucleotide strand comprises a polynucleotide strand and a hydrophobic moiety; wherein each of the plurality of staple polynucleotide strands hybridises to the at least one scaffold polynucleotide strand to form the three-dimensional structure of the membrane-spanning nanopore, and wherein the at least one hydrophobically-modified polynucleotide strand hybridises to a portion of the at least one scaffold polynucleotide strand, the membrane-spanning nanopore defining a central channel with a minimum internal width of at least about 5 nm. Membranes comprising the membrane-spanning nanopore and applications of those membranes are also provided.Type: ApplicationFiled: July 14, 2017Publication date: October 10, 2019Applicant: UCL BUSINESS PLCInventors: Stefan HOWORKA, Genevieve PUGH, Jonathan Richard BURNS
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Patent number: 10400014Abstract: The invention relates to mutant forms of the outer membrane-located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.Type: GrantFiled: September 1, 2015Date of Patent: September 3, 2019Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20180148481Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: September 1, 2015Publication date: May 31, 2018Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20170283470Abstract: The invention relates to mutant forms of the outer membrane located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.Type: ApplicationFiled: September 1, 2015Publication date: October 5, 2017Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20070087382Abstract: The invention relates to assemblies for bonding molecules comprising bondable functional groups, which are present on a solid supporting material as individual molecular functional groups or multiple identical functional groups. Said assemblies are characterised in that the density of the individual functional groups or multiple functional groups on the solid supporting material is between 104 and 1010 individual or multiple functional groups per cm2 and that there are no additional bondable functional groups within a selected distance d from any individual bondable functional group or multiple functional group for at least 95% and in particular at least 99% of the individual or multiple functional groups.Type: ApplicationFiled: September 10, 2004Publication date: April 19, 2007Applicant: Upper Austrian Research GmbHInventors: Stefan Howorka, Patrick Pammer
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Publication number: 20050208574Abstract: Provided are pore-subunit polypeptides covalently linked to one or more sensing moieties, and uses of these modified polypeptides to detect and/or measure analytes or physical characteristics within a given sample.Type: ApplicationFiled: May 16, 2005Publication date: September 22, 2005Inventors: Hagan Bayley, Stefan Howorka, Liviu Movileanu
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Publication number: 20040265936Abstract: The invention concerns a process for the recombinant production of S-layer proteins in gram-negative host cells. Furthermore the nucleotide sequence of a new S-layer gene and processes for the production of modified S-layer proteins are disclosed.Type: ApplicationFiled: July 14, 2004Publication date: December 30, 2004Applicants: NANO-S Biotechnologie GmbHInventors: Werner Lubitz, Uwe Sleytr, Beatrix Kuen, Michaela Truppe, Stefan Howorka, Stepanka Resch, Gerhard Schroll, Margit Sara
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Patent number: 6777202Abstract: The invention concerns a process for the recombinant production of S-layer proteins in gram-negative host cells. Furthermore the nucleotide sequence of a new S-layer gene and processes for the production of modified S-layer proteins are disclosed.Type: GrantFiled: December 2, 1998Date of Patent: August 17, 2004Assignees: NANO-S Biotechnologie GmbHInventors: Werner Lubitz, Uwe Sleytr, Beatrix Kuen, Michaela Truppe, Stefan Howorka, Stepanka Resch, Gerhard Schroll, Margit Sara
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Publication number: 20020168728Abstract: The invention concerns a process for the recombinant production of S-layer proteins in gram-negative host cells. Furthermore the nucleotide sequence of a new S-layer gene and processes for the production of modified S-layer proteins are disclosed.Type: ApplicationFiled: December 2, 1998Publication date: November 14, 2002Applicant: ARENT FOX KINTNER PLOTKIN AND KAHN, PLLCInventors: WERNER LUBITZ, UWE SLEYTR, BEATRIX KUEN, MICHAELA TRUPPE, STEFAN HOWORKA, STEPANKA RESCH, GERHARD SCHROLL, MARGIT SARA