Patents by Inventor Stefan Pitsch
Stefan Pitsch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230158469Abstract: Provided herein are compositions, devices, systems and methods for DNA oligomer synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.). The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.Type: ApplicationFiled: October 31, 2022Publication date: May 25, 2023Inventors: Jeremy LACKEY, David DODD, Stefan PITSCH
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Publication number: 20230147145Abstract: The invention relates to a compound characterized by general formula (100), wherein R1 and R6 are H or F, R2, R3, R4 and R5 can be any substituent, R7, R8, RN1, RN2, RN3 and RN4 are a hydrocarbon moiety, one of R9 and R10 is hydrogen and the other one is hydrogen or a saturated carbon atom connected to any substituent, and its use in staining and live cell fluorescence imaging.Type: ApplicationFiled: December 20, 2018Publication date: May 11, 2023Applicant: SPIROCHROME AGInventors: Kai JOHNSSON, Luc REYMOND, Michelle FREI, Stefan PITSCH
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Publication number: 20220410074Abstract: A method is presented for checking the integrity of a hollow-fibre fluid filter, in particular a hollow-fibre dialyzer (1), which is constructed from a plurality of hollow fibres (15) enclosed by a membrane, with the steps of: perfusing the inside or outside of the hollow fibres (15) with a fluid, supplying the outside or inside of the hollow fibres (15) with a gas, wherein the gas has a higher pressure than the fluid, and determining a quantity of the gas which penetrates into the fluid through holes in the membrane. The method is characterized in that, after flowing through the hollow-fibre fluid filter (1), the fluid is channelled through a bubble trap (30), and in that a volume of gas (41) collecting in the bubble trap (30) during a predefined or predefinable reference period is determined. Furthermore, equipment for checking the integrity of a hollow-fibre fluid filter (1) is presented.Type: ApplicationFiled: November 3, 2020Publication date: December 29, 2022Applicant: FRESENIUS MEDICAL CARE DEUTSCHLAND GMBHInventors: Stefan PITSCH, Manuel WILHELM
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Publication number: 20220032256Abstract: Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable LED arrays to control polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.Type: ApplicationFiled: July 6, 2021Publication date: February 3, 2022Inventors: Jeremy LACKEY, Scott INDERMUEHLE, David DODD, Stefan PITSCH
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Publication number: 20200222875Abstract: Methods, systems, compositions, and devices for the manufacturing of high-quality building blocks, such as polynucleotides, are described herein. Processes described herein provide for efficient washing of residual reagents, solvents, or byproducts from previous synthetic steps to allow for the generation of polynucleotides with low error rates. Processes described herein also provide for reduction in deletion rates during chemical nucleic acid synthesis. Further, methods and devices described herein allow for the rapid construction and assembly of large libraries of highly accurate polynucleotides.Type: ApplicationFiled: December 23, 2019Publication date: July 16, 2020Inventors: Bill James PECK, Mary NOE, Stefan PITSCH, Patrick A. Weiss
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Patent number: 9534003Abstract: The invention features protected linker compounds which comprise at one terminus a protected amino group and at another other terminus a phosphorous activating group, such as a phosphoramidite group. These protected linker compounds are introduced chemically at the 5?-end of oligonucleotides for the purpose of preparing 5?-amino modified oligonucleotides. After deprotection, the thereby introduced amino group then allows further modification (e.g. attachment of dyes) or immobilization (on surfaces or beads) of the oligonucleotide. Specifically, the presented amino protecting group is designed to provide such protected linker compounds in a solid form, which facilitates efficient purification by precipitation or crystallization and aliquoting for distribution and storage.Type: GrantFiled: December 19, 2012Date of Patent: January 3, 2017Assignee: ReseaChem GmbHInventors: Stefan Pitsch, Stefan Berger
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Publication number: 20150011746Abstract: The invention features protected linker compounds which comprise at one terminus a protected amino group and at another other terminus a phosphorous activating group, such as a phosphoramidite group. These protected linker compounds are introduced chemically at the 5?-end of oligonucleotides for the purpose of preparing 5?-amino modified oligonucleotides. After deprotection, the thereby introduced amino group then allows further modification (e.g. attachment of dyes) or immobilization (on surfaces or beads) of the oligonucleotide. Specifically, the presented amino protecting group is designed to provide such protected linker compounds in a solid form, which facilitates efficient purification by precipitation or crystallization and aliquoting for distribution and storage.Type: ApplicationFiled: December 19, 2012Publication date: January 8, 2015Inventors: Stefan Pitsch, Stefan Berger
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Patent number: 8859749Abstract: The present invention refers to a double-stranded siRNA molecule comprising a sense Strand and an antisense Strand which is essentially complementary to the sense Strand, each of the sense and the antisense Strands comprising at least 17 nucleotides (nt), the siRNA further comprising at least one overhang at the 5? and/or 3? end, wherein the overhang residue or overhang residues are chemically modified and selected independently from each other from the group consisting of: (a) 2?-deoxy modified nucleotides; (b) 2?-methoxy modified nucleotides; (c) two nucleosides linked by a 3? to 5? or 2? to 5? formacetal linkage; (d) nucleotides modified at the 2?-position by a —O—CH2—O—(CH2)2—OH group; and (e) nucleotides comprising in the 3?-position a —CH2—O—(CH2)7—CH3 group.Type: GrantFiled: March 8, 2006Date of Patent: October 14, 2014Assignee: QIAGEN GmbHInventors: Ioanna Andreou, Stefan Pitsch, Evelyne Muller, Luc Reymond
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Patent number: 7772439Abstract: An amino or thiol linker building block for the synthesis of amino or thiol functionalized amino acids and generally of the following structure: is provided. Such building block may be introduced in the 5? end position of an amino acid under standard coupling conditions. Such building block allows in-line coupling control, “trityl-on” purification, and solid support functionalization/derivatization. Such building block is a stable, solid compound and can therefore be easily handled. With the building block of the present invention, deprotection may be carried out under standard detritylation conditions.Type: GrantFiled: October 25, 2005Date of Patent: August 10, 2010Assignee: Operon Biotechnologies, Inc.Inventors: Patrick A. Weiss, Violette Y. Weiss, Stefan Pitsch
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Publication number: 20090306177Abstract: Double-stranded short interfering ribonucleic acid (siRNA) are modified to reduce or eliminate their immunostimulatory effect without significantly affecting their gene silencing effect. Modified siRNA include one or more 2? sugar modifications and, optionally, internucleotide linkages on the sense strand. Compositions containing the modified siRNA and methods of making and using the modified siRNA are disclosed. New and previously characterized siRNA can be synthesized to incorporate modifications according to the invention.Type: ApplicationFiled: September 15, 2006Publication date: December 10, 2009Applicants: Coley Pharmaceutical GmbH, Qiagen GmbHInventors: Eugen Uhlmann, Marion Jurk, Joerg Vollmer, Christian Schetter, Martin Weber, Ioanna Andreou, Stefan Pitsch
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Publication number: 20090197332Abstract: The present invention refers to a double-stranded siRNA molecule comprising a sense Strand and an antisense Strand which is essentially complementary to the sense Strand, each of the sense and the antisense Strands comprising at least 17 nucleotides (nt), the siRNA further comprising at least one overhang at the 5? and/or 3? end, wherein the overhang residue or overhang residues are chemically modified and selected independently from each other from the group consisting of: (a) 2?-deoxy modified nucleotides; (b) 2?-methoxy modified nucleotides; (c) two nucleosides linked by a 3? to 5? or 2? to 5? formacetal linkage; (d) nucleotides modified at the 2?-position by a -0-CH2—O—(CH2)2—OH group; and (e) nucleotides comprising in the 3?-position a —CH2—O—(CH2)7—CH3 group.Type: ApplicationFiled: March 8, 2006Publication date: August 6, 2009Inventors: Ioanna Andreou, Stefan Pitsch, Evelyne Muller, Luc Reymond
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Publication number: 20060167240Abstract: An amino or thiol linker building block for the synthesis of amino or thiol functionalized amino acids and generally of the following structure: is provided. Such building block may be introduced in the 5? end position of an amino acid under standard coupling conditions. Such building block allows in-line coupling control, “trityl-on” purification, and solid support functionalization/derivatization. Such building block is a stable, solid compound and can therefore be easily handled. With the building block of the present invention, deprotection may be carried out under standard detritylation conditions.Type: ApplicationFiled: October 25, 2005Publication date: July 27, 2006Applicant: Operon Holdings, Inc.Inventors: Patrick Weiss, Violette Weiss, Stefan Pitsch
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Patent number: 6545134Abstract: Processes for the preparation of 3′,4′-cyclic acetals of pentopyranosylnucleosides, in which the pentopyranosyl nucleoside is reacted with an aldehyde, ketone, acetal, or ketal under reduced pressure of less than about 500 mbar.Type: GrantFiled: January 18, 2001Date of Patent: April 8, 2003Assignee: Nanogen Recognomics, GmbH.Inventors: Albert Eschenmoser, Stefan Pitsch, Sebastian Wendeborn
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Patent number: 5986084Abstract: The ribonucleoside-derivatives serve for the synthesis of ribonucleic acids and comprise a triple substituted silyloxymethyl-group as a protection-group on the oxygen atom in 2'-position. The ribonucleoside-derivatives may be suitably protected on the nucleo-base and on the oxygen in 5'-position also. The new protection-groups in 2'-O-position are superior to conventional such protection-groups as they are not subject to isomerization and give higher coupling yields. The general formula of the ribonucleoside-derivative is: ##STR1## whereby R.sup.1 is a base of the purine- or pyrimidine-family or a derivative of such a base,R.sup.2 is a proton or a substituted derivative of phosphonic acid,R.sup.3 is a proton or a suitable protection-group,R.sup.4, R.sup.5, R.sup.6 are advantageously three identical or different alkyl- or aryl-substituents which together comprise between 6 and 30 carbon atoms.Type: GrantFiled: November 7, 1997Date of Patent: November 16, 1999Inventors: Stefan Pitsch, Patrick A. Weiss, Luzi Jenny