Patents by Inventor Stefan Schoenert

Stefan Schoenert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS OF RECOMBINANTLY PRODUCING NEUTRAL PROTEASE ORIGINATING FROM PAENIBACILLUS POLYMXA
    Publication number: 20240132866
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Application
    Filed: November 22, 2022
    Publication date: April 25, 2024
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • ONE-POT CELL-FREE GLYCOSYLATION PROCESS
    Publication number: 20230304055
    Abstract: The invention relates to a process for the preparation of a glycosylated organic compound by in vitro glycosylation of an organic compound having a nucleophilic group with a saccharide under catalysis of a Leloir glycosyltrans-ferase system comprising at least a first glycosyl transferase and a second glycosyl transferase. The invention further relates to a composition comprising a glycosylated organic compound that is obtainable by the process according to the invention.
    Type: Application
    Filed: June 29, 2021
    Publication date: September 28, 2023
    Applicant: C-LEcta GmbH
    Inventors: Andreas VOGEL, Claudia FELLER, Christopher David BAYER, Gregor HOFFMANN, Stefan SCHOENERT
  • Expression vector encoding a neutral protease
    Patent number: 11542489
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Grant
    Filed: July 27, 2021
    Date of Patent: January 3, 2023
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Enzyme products
    Patent number: 11286474
    Abstract: The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: March 29, 2022
    Assignee: C-LECTA GMBH
    Inventors: Stefan Schoenert, Mathias Salomo, Thomas Schultchen, Andreas Vogel, Sabrina Koepke, Sebastian Bartsch, Birgit Brucher, Claudia Feller
  • METHODS OF RECOMBINANTLY PRODUCING NEUTRAL PROTEASE ORIGINATING FROM PAENIBACILLUS POLYMXA
    Publication number: 20210355472
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Application
    Filed: July 27, 2021
    Publication date: November 18, 2021
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Methods of recombinantly producing neutral protease originating from
    Patent number: 10889809
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: January 12, 2021
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • HIGH-PURITY STEVIOL GLYCOSIDES
    Publication number: 20200157594
    Abstract: Methods of preparing highly purified steviol glycosides, particularly rebaudiosides M, D, E and I are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
    Type: Application
    Filed: May 15, 2018
    Publication date: May 21, 2020
    Inventors: Avetik MARKOSYAN, Siddhartha PURKAYASTHA, Christopher BAYER, Andreas VOGEL, Sabrina KÖPKE, Sebastian BARTSCH, Birgit BRUCHER, Claudia FELLER, Stefan SCHÖNERT, Mathias SALOMO, Thomas SCHULTCHEN
  • ENZYME PRODUCTS
    Publication number: 20200140838
    Abstract: The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.
    Type: Application
    Filed: November 13, 2019
    Publication date: May 7, 2020
    Inventors: Stefan SCHOENERT, Mathias SALOMO, Thomas SCHULTCHEN, Andreas VOGEL, Sabrina KOEPKE, Sebastian BARTSCH, Birgit BRUCHER, Claudia FELLER
  • METHODS OF RECOMBINANTLY PRODUCING NEUTRAL PROTEASE ORIGINATING FROM PAENIBACILLUS POLYMYXA
    Publication number: 20200115692
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Application
    Filed: December 2, 2019
    Publication date: April 16, 2020
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Recombinantly produced neutral protease originating from Paenibacillus polymyxa
    Patent number: 10526594
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: January 7, 2020
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • METHODS OF RECOMBINANTLY PRODUCING NEUTRAL PROTEASE ORIGINATING FROM PAENIBACILLUS POLYMYXA
    Publication number: 20190203191
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Application
    Filed: March 15, 2019
    Publication date: July 4, 2019
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Method for producing serratia marcescens nuclease using a bacillus expression host
    Patent number: 9796994
    Abstract: A method for producing a nuclease of a gram negative bacterium or a nuclease preparation containing a nuclease of a gram negative bacterium including expression of the nuclease in a gram positive bacterium and subsequent secretion of the nuclease, as well as a nuclease or a nuclease preparation that can be obtained by this method.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: October 24, 2017
    Assignee: c-LEcta GmbH
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • RECOMBINANTLY PRODUCED NEUTRAL PROTEASE ORIGINATING FROM PAENIBACILLUS POLYMYXA
    Publication number: 20170037390
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Application
    Filed: October 26, 2016
    Publication date: February 9, 2017
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • RECOMBINANTLY PRODUCED NEUTRAL PROTEASE ORIGINATING FROM PAENIBACILLUS POLYMYXA
    Publication number: 20150166973
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Application
    Filed: January 9, 2015
    Publication date: June 18, 2015
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Method for Producing Nucleases of a Gram Negative Bacterium While Using a Gram Positive Expression Host
    Publication number: 20120135498
    Abstract: A method for producing a nuclease of a gram negative bacterium or a nuclease preparation containing a nuclease of a gram negative bacterium including expression of the nuclease in a gram positive bacterium and subsequent secretion of the nuclease, as well as a nuclease or a nuclease preparation that can be obtained by this method.
    Type: Application
    Filed: February 2, 2012
    Publication date: May 31, 2012
    Applicant: c-LEcta GmbH
    Inventors: Thomas GREINER-STOEFFELE, Stefan Schoenert