Abstract: The present invention provides peptides useful as epitope tags, which may be fused to a polypeptide of interest, as well as antibodies that specifically bind to these peptides. The peptides and/or antibodies can be used for detecting, immobilizing, isolating or purifying a molecule that is conjugated to such a peptide and/or antibody.

Abstract: The present invention provides peptides useful as epitope tags, which may be fused to a polypeptide of interest, as well as antibodies that specifically bind to these peptides. The peptides and/or antibodies can be used for detecting, immobilizing, isolating or purifying a molecule that is conjugated to such a peptide and/or antibody.

Abstract: The present invention provides peptides useful as epitope tags, which may be fused to a polypeptide of interest, as well as antibodies that specifically bind to these peptides. The peptides and/or antibodies can be used for detecting, immobilizing, isolating or purifying a molecule that is conjugated to such a peptide and/or antibody.

Abstract: The present invention relates to a fusion protein, comprising the structure N-PCSY-degSigN-M-PCSX-degSigC-C; wherein N represents the N-terminus; PCSY and PCSX each represent a protease cleavage site (PCS), which differ from each other in at least one amino acid residue: degSigN represents a degradation signal which promotes degradation of the fusion protein in a host cell if PCSY is cleaved by a protease such that the first amino acid of degSigN becomes the new N-terminus of the remaining fusion; M represents a cytoplasmic selection marker; and degSigC represents a second degradation signal which promotes degradation of the fusion protein in a host cell if PCSX is not cleaved by a protease; and C represents the C-terminus.

Abstract: The present invention provides peptides useful as epitope tags, which may be fused to a polypeptide of interest, as well as antibodies that specifically bind to these peptides. The peptides and/or antibodies can be used for detecting, immobilizing, isolating or purifying a molecule that is conjugated to such a peptide and/or antibody.

Abstract: The present invention relates to a fusion protein, comprising the structure N-PCSY-degSigN-M-PCSX-degSigCC; wherein N represents the N-terminus; PCSY and PCSX each represent a protease cleavage site (PCS), which differ from each other in at least one amino acid residue; degSigN represents a degradation signal which promotes degradation of the fusion protein in a host pot cell if PCSY is cleaved by a protease such that the first amino acid of degSigN becomes the new N-terminus of the remaining fusion; M represents a cytoplasmic selection marker; and degSigC represents a second degradation signal which promotes degradation of the fusion protein in a host cell if PCSX is not cleaved by a protease; and C represents the C-terminus.

Abstract: The invention relates to methods of detecting a target antigen by optical detection, isotopic detection, or detection by electron microscopy, comprising contacting a first or second antibody with at least one monovalent antibody that specifically binds said first or second antibody, wherein the monovalent antibody has one or more fluorescent label(s), chromophore label(s), isotope label(s), or metal label(s) attached to it. The invention also relates to complexes formed by the methods of the invention, kits for performing the method of the invention, monovalent antibodies useful for conducting the methods of the invention, as well as uses of the monovalent antibody according to the methods of the invention.

Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Abstract: The present invention belongs to the field of biotechnology. More specifically, the present invention provides a protease, a non-naturally occurring fusion protein comprising a corresponding protease recognition site, expression vectors encoding same, host cells comprising said expression vectors, kit of parts as well as methods applying the protease, fusion protein, and uses thereof, as defined in the claims. The presently disclosed protease/protease recognition site is particularly useful in methods requiring an orthogonal set of proteases, and is suitable for use in both prokaryotic and selected eukaryotic expression systems.

Abstract: The present invention belongs to the field of biotechnology. More specifically, the present invention provides a protease, a non-naturally occurring fusion protein comprising a corresponding protease recognition site, expression vectors encoding same, host cells comprising said expression vectors, kit of parts as well as methods applying the protease, fusion protein, and uses thereof, as defined in the claims.

Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Abstract: A blow molding tool (1) for producing a plastic article, in particular a fuel tank, comprising at least one clamping frame (2) with mold clamping devices (3) and at least two blow mold halves, which are formed such that they can be moved together and apart by means of an opening and closing movement of the clamping frame (2) and which respectively have at least one cavity (8) for the forming of the plastic article, the blow molding tool (1) being distinguished by the fact that the mold clamping devices (3) respectively have at least one tool holder (6), in which a mold insert (7) is in each case exchangeably inserted, and in that the tool holders (6) respectively form a peripheral sealing edge (10).

Abstract: A blow molding tool (1) for producing a plastic article, in particular a fuel tank, comprising at least one clamping frame (2) with mold clamping devices (3) and at least two blow mold halves, which are formed such that they can be moved together and apart by means of an opening and closing movement of the clamping frame (2) and which respectively have at least one cavity (8) for the forming of the plastic article, the blow molding tool (1) being distinguished by the fact that the mold clamping devices (3) respectively have at least one tool holder (6), in which a mold insert (7) is in each case exchangeably inserted, and in that the tool holders (6) respectively form a peripheral sealing edge (10).

Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Abstract: A plurality of validated electronic trade documents, associated with a transaction between a government agency buyer and a government agency seller, are obtained. The electronic trade documents are logically linked in an intragovernmental transaction container, and periodically scanned to determine if conditions exist for at least partial settlement of the transaction. Responsive to the determination that the conditions for the at least partial settlement of the transaction exist, the at least partial settlement of the transaction is initiated. Capability for handling grants can also be provided.