Abstract: The invention provides a method of treating a polyvinyl difluoride (PVDF) membrane comprising: (a) contacting said membrane with an alcohol and a wetting agent; and (b) drying said membrane.

Abstract: A method of detecting the production of an analyte by cells in a sample, which method comprises: (i) providing an antibody capable of binding to said analyte and a first agent capable of enhancing detection of said analyte, wherein said antibody and said first agent are immobilized on the same support; (ii) contacting said antibody and said agent with a sample of cells; and (iii) detecting binding of said analyte to said antibody thereby detecting the production of an analyte by cells in a sample.

Abstract: The invention provides a method for detecting the production of one or more analytes by cells in a sample. The method uses one or more immobilized binding proteins to capture one or more analytes released from cells. The, or each, binding protein is localized at one or more discrete locations on a solid surface within a well. Cells are introduced into the well and cultured under conditions suitable for release of the analyte(s) and for binding of the analyte(s) to the binding protein(s). Restricting the binding protein(s) to one or more discrete locations within the well, rather than coating the entire surface of the well, results in increased sensitivity of the assay.

Abstract: The invention provides a method of treating a polyvinyl difluoride (PVDF) membrane comprising: (a) contacting said membrane with an alcohol and a wetting agent; and (b) drying said membrane.

Abstract: The invention provides a method of treating a polyvinyl difluoride (PVDF) membrane comprising: (a) contacting said membrane with an alcohol and a wetting agent; and (b) drying said membrane.

Abstract: A method of detecting the production of an analyte by cells in a sample, which method comprises: (i) providing an antibody capable of binding to said analyte and a first agent capable of enhancing detection of said analyte, wherein said antibody and said first agent are immobilised on the same support; (ii) contacting said antibody and said agent with a sample of cells; and (iii) detecting binding of said analyte to said antibody thereby detecting the production of an analyte by cells in a sample.

Abstract: The invention provides a method for detecting the production by cells in a sample of one or more analyte, which method comprises: (a) providing (i) a first binding protein which specifically binds to a first analyte; and (ii) optionally a second binding protein which specifically binds to second analyte; wherein said first binding protein and optionally said second binding protein are each immobilized at one or more discrete location within a well; (b) introducing a sample of cells into said well; (c) culturing said sample of cells under conditions suitable for: (i) release of said analyte and optionally said second analyte and optionally said one or more further analyte by said cells; and (ii) binding of said first analyte to said first binding protein and optionally binding of said second analyte to said second binding protein; and (d) determining whether or not said first analyte is bound to said first binding protein and whether or not said second analyte is bound to said second binding protein, thereby d