Abstract: The invention relates to a method for producing transparent tissue samples of a biological or human tissue for a light microscopy examination, having the steps a) dewatering the tissue sample using a dewatering solvent and b) clearing the dewatered tissue sample by transferring same into an embedding medium which contains an benzaldehyde-anisole ether. In comparison to the benzyl benzoate/benzyl alcohol mixtures and methyl salicylate-benzyl benzoate mixtures conventionally used until now as embedding media, the benzaldehyde-anisole ether has the advantage of being usable as a pure substance, in exactly the same way as a dibenzyl ether: Because the pure substance already has the desired refractive index, the refractive index does not need to be set by mixing the embedding medium. Furthermore, the benzaldehyde-anisole ether according to the invention penetrates the dewatered tissue faster than previously known embedding media and also makes the tissue transparent faster.

Abstract: The invention relates to a method for producing transparent tissue samples of a biological or human tissue for a light microscopy examination, having the steps a) dewatering the tissue sample using a dewatering solvent and b) clearing the dewatered tissue sample by transferring same into an embedding medium which contains an aromatic ester. The aromatic ester is a phenylacetic acid ester. In comparison to the benzyl benzoate/benzyl alcohol mixtures and methyl salicylate-benzyl benzoate mixtures conventionally used until now as embedding media, the aromatic ester has the advantage of being usable as a pure substance, in exactly the same way as a dibenzyl ether: Because the pure substance already has the desired refractive index, the refractive index does not need to be set by mixing the embedding medium. Furthermore, the aromatic ester is non-toxic, has a low degree of volatility, and allows a cold storage of the transparent tissue.

Abstract: The invention relates to an electrophoresis device (1) for use in a method for producing transparent biological samples (2), comprising a reaction frame (3), the reaction frame (3) having an open top side (4) and a bottom side (5) opposite the top side (4), characterized in that the bottom side (5) has at least partially one opening (6). The invention also relates to a use of a sample cassette (19) for an electrophoresis method.

Abstract: In accordance with the invention, a method for executing reactions at temperatures exceeding 50.degree. C. is disclosed using minimal quantities of fluid samples. The method uses a reaction vessel which minimizes the containment space for the fluid sample. The reaction vessel includes a sample-receiving tube and an insert. The insert engages the sample-receiving tube, forming a seal and prevents evaporation of the fluid sample during heating. The sample-receiving tube of the method is tapered to be narrow towards the bottom so that the insert can be easily inserted into the sample-receiving tube from the top and provide a tight seal in the lower extremity without increasing pressure in the reaction chamber. Equalization between the two sides of the sealing insert can occur as the insert is being introduced. Minimal displacement of the insert in the axial direction of the sample-receiving tube produces a sealing connection minimizing undesirable pressure build up.

Abstract: A column unit is provided consisting of a centrifuging vessel and a receiving body with feed and discharge openings located at opposite ends. Desired column material is located within a middle portion of a hollow cylinder of the receiving body. A portion of the receiving body containing the discharge opening and the column material is received by the centrifuging vessel. The entire column unit is inserted into a conventional stand in a centrifuge. Accordingly, when sample material is introduced into the receiving body through the feed opening, it may flow without misdirection through the column material and discharge opening and into the centrifuge vessel.

Abstract: The present invention provides a device for the elution of electrically-charged macromolecules, especially biopolymers, from a gel by electrophoretic transfer of the macromolecules from a gel placed under a buffer into a salt solution, said device having a container (2) for the reception of an electrolyte, which chamber is divided up by at least one reception chamber for a piece of gel, which reception chamber is permeable to the electrolyte, into two electrode chambers (12, 14), each of which contains at least one electrode (8, 10), wherein the electrode chambers (12, 14) are separated from one another by a block (4) provided with a closable electrolyte passage (6) and the at least one reception chamber for a piece of gel is formed by a trough (16) let into the surface of the block (4), which trough is connected via at least one first opening (20), present in or next to the bottom (18) thereof, with one electrode chamber (14) and via a second opening (22), present in or next to the bottom thereof with a cana