Abstract: A method of contrast enhancement of a three-dimensional light sheet microscopy image formed from N individual images each corresponding to a light sheet plane and spaced apart from each other in the z-direction by at least a distance d, the x/y-plane being the light sheet plane and the x-direction being the propagation direction of the light sheet of the light sheet plane, comprising: Deconvolution of the three-dimensional image in the z-direction, comprising: For each intensity vector of N intensities (Ix,y,1, . . . ,Ix,y,N) having the same x/y value, performing a multiplication with a tridiagonal N×N deconvolution matrix, which assigns to each voxel (x, y, n) a correction parameter f1, with which, by the multiplication of the deconvolution matrix with the intensity vector, for a component I(x, y, n) of the intensity vector the intensities Ix, y,n+1 and Ix, y,n?1 of the corresponding voxels of the neighboring image plane are multiplied, before they are subtracted from the intensity value I(x, y,n).