Abstract: Disclosed is a method for producing genetically engineered cells in vitro. The method includes direct injection of a genome editing composition into a cell nucleus of a cell. The genome editing composition includes at least one Cas protein and at least one gRNA molecule to target a distinct genomic location. The injection is performed with a microelectromechanical systems injection chip including a cantilever. The cantilever includes a microchannel being in fluid communication with a nanosyringe, and wherein direct injection includes providing a fluid communication between the microchannel and the nucleus of the cell by insertion of the nanosyringe into the nucleus of the cell and injecting the genome editing composition via the microchannel through the nanosyringe into the nucleus of the cell.

Abstract: A scanning probe microscopy (SPM) system includes a sample stage and one or more sample motion stages for actuating the sample stage, a SPM probe and one or more probe motion stages for actuating the SPM probe and for performing an SPM scan of a sample on the sample stage, and a system controller. The system controller includes an image acquisition module to collect from an image acquisition device one or more images of the sample carried by the sample stage, a touch-screen control module to display the one or more images of the sample together with one or more tools on a touch-screen and to generate one or more control actions depending on the detection of a gesture of a user touching the touchscreen, and a control module to receive one or more control actions generated by the touch-screen control module and to control the SPM system according to the received control actions.