Abstract: Methods, devices, reagents and kits designed to improve the performance of proteomic based assays are provided. Such methods have a wide utility in proteomic applications for research and development, diagnostics and therapeutics by providing for a reduction or elimination of background signal and improved specificity for protein binding reagents in a multiplex assay formats.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced or eliminated while maintaining the aptamer-target interaction.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced or eliminated while maintaining the aptamer-target interaction.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced or eliminated while maintaining the aptamer-target interaction.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

Abstract: Embodiments provide a slide assembly device having a static tooling base which is statically and solidly affixed to a base such as a table and a moveable tooling arm that is rotatable about a hinge connected to the static tooling base, so that moveable tooling arm rotates about the hinge in a manner similar to a book cover opening and closing. The embodiments further provide an upper slide chuck that is removably attachable to the moveable tooling arm and a lower slide receiver that is removably attachable to the static tooling base. The upper slide chuck is configured to hold an experimental slide via a vacuum mechanism to engagedly hold the experimental slide to the upper slide chuck while the moveable tooling arm is rotated about the hinge from an open-book position to a closed-book position.

Abstract: Embodiments provide a slide assembly device having a static tooling base which is statically and solidly affixed to a base such as a table and a moveable tooling arm that is rotatable about a hinge connected to the static tooling base, so that moveable tooling arm rotates about the hinge in a manner similar to a book cover opening and closing. The embodiments further provide an upper slide chuck that is removably attachable to the moveable tooling arm and a lower slide receiver that is removably attachable to the static tooling base. The upper slide chuck is configured to hold an experimental slide via a vacuum mechanism to engagedly hold the experimental slide to the upper slide chuck while the moveable tooling arm is rotated about the hinge from an open-book position to a closed-book position.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced or eliminated while maintaining the aptamer-target interaction.

Abstract: Embodiments provide a slide assembly device having a static tooling base which is statically and solidly affixed to a base such as a table and a moveable tooling arm that is rotatable about a hinge connected to the static tooling base, so that moveable tooling arm rotates about the hinge in a manner similar to a book cover opening and closing. The embodiments further provide an upper slide chuck that is removably attachable to the moveable tooling arm and a lower slide receiver that is removably attachable to the static tooling base. The upper slide chuck is configured to hold an experimental slide via a vacuum mechanism to engagedly hold the experimental slide to the upper slide chuck while the moveable tooling arm is rotated about the hinge from an open-book position to a closed-book position.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

Abstract: Embodiments provide a slide assembly device having a static tooling base which is statically and solidly affixed to a base such as a table and a moveable tooling arm that is rotatable about a hinge connected to the static tooling base, so that moveable tooling arm rotates about the hinge in a manner similar to a book cover opening and closing. The embodiments further provide an upper slide chuck that is removably attachable to the moveable tooling arm and a lower slide receiver that is removably attachable to the static tooling base. The upper slide chuck is configured to hold an experimental slide via a vacuum mechanism to engagedly hold the experimental slide to the upper slide chuck while the moveable tooling arm is rotated about the hinge from an open-book position to a closed-book position.

Abstract: A method and a device for producing a metal strip by continuous casting and rolling, in which a thin slab is initially cast into a casting machine, and is subsequently rolled in at least one rolling train using primary heat from the casting cycle. The cast thin slab is passed between the casting machine and the at least one rolling train and at least one holding oven as well as at least one induction oven. The holding oven and the induction oven are activated or deactivated according to a selected mode of operation, that is, a first mode of operation for the continuous production of the metal strip and a second mode of operation for the discontinuous production of the metal strip.

Abstract: A method of manufacturing a metal strip includes casting a slab (3) or preliminary strip (3?) in a casting machine (2) and subsequently rolling the cast slab (3) or preliminary strip (3?) in at least one rolling train (4, 5) in a first operating mode with the at least one rolling train being connected with the casting machine for continuously manufacturing the metal strip, and in a second operating mode removing the cast slab (3) or preliminary strip (3?) from the main transport line (6) with a shuttle system arranged between the casting machine (2) and the at least one rolling train (4, 5), storing the removed slab (3) or preliminary strip (3?), and subsequently transporting the removed slab (3) or preliminary strip back into the main transport line, with the removed slab (3) or preliminary strip (3?) being heated to a desired temperature or maintained at a desired temperature prior to the transport back into the main transport line (6) for discontinuously manufacturing the metal strip.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

Abstract: The invention relates to a method for producing a metal strip (1) by continuous casting and rolling. According to said method, a thin slab (3) is initially cast into a casting machine (2), which is subsequently rolled in at least one rolling train (4, 5) using primary heat from the casting cycle. According to the invention, in order to improve the functionality of the continuous casting and rolling installation, the cast thin slab (3) is passed between the casting machine (2) and the at least one rolling train (4, 5) and at least one holding oven (6) as well as at least one induction oven (7). The holding oven (6) and the induction oven (7) are activated or deactivated according to a selected mode of operation, that is, a first mode of operation for the continuous production of the metal strip (1) and a second mode of operation for the discontinuous production of the metal strip (1). The invention further relates to a device for producing a metal strip by continuous casting and rolling.

Abstract: The invention pertains to a method for manufacturing a metal strip (1) by means of continuous casting and rolling, wherein a thin slab (3) is initially cast in a casting machine (2) and this thin slab is subsequently rolled in at least one rolling train (4, 5) by utilizing the primary heat of the casting process, wherein a continuous manufacture of the metal strip (1) (continuous rolling) can be realized in a first operating mode by directly coupling the casting machine (2) to the at least one rolling train (4, 5), and wherein a discontinuous manufacture of the metal strip (1) (batch rolling) can be realized in a second operating mode by decoupling the casting machine (2) from the at least one rolling train (4, 5).

Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

Abstract: In a fuel supply system for a spark-ignition internal combustion engine having a blow-in valve for each cylinder for blowing an easily ignitable mixture of a liquid fuel and a combustion gas via a blow-in nozzle into a combustion chamber of the internal combustion engine, the blow-in valve has a pressure connection for supplying pressurized combustion gas to a blown-in nozzle from an external pressure source, and the blow-in valve has a piston/cylinder unit for delivering the liquid fuel for injection into the cylinder together with the combustion gas, with a plunger being provided for an at least indirect actuation of a nozzle needle of the blow-in nozzle controlling the injection of a metered quantity of fuel and the combustion gas into the combustion chamber.

Abstract: In a method for operating a spark-ignition internal combustion engine, wherein fuel is metered into the cylinders by direct fuel injection into the combustion chambers and by the addition of fuel in an intake region of the engine and a control unit matches the quantity of fuel to be added in the intake region and the quantity of fuel to be injected directly into the combustion chamber to one another as a function of the operating point of the internal combustion engine so as to provide an ignitable fuel mixture in the combustion chamber, the control unit determines a recirculation rate for the re-circulated exhaust gas and adjusts a predetermined excess air ratio (?) for the mixture composed of fresh air, re-circulated exhaust gas and fuel by means of the direct injection of fuel into the combustion chamber taking into account the recirculation rate in order to reduce the exhaust emissions of the internal combustion engine and improve fuel consumption.