Abstract: The invention relates to a method for protecting transplants, as well as organs and individual blood vessels, against inflammatory reactions that are caused by the operative procedure linked to the explantation and storage until transplantation. This can be achieved by treating the transplant via intra arterial application of flavonol compounds, in particular with quercetin glucuronide and/or kaempferol glucuronide.

Abstract: The present invention relates to the use of flavonoid compounds, particularly quercetin-3-O-?-D-glucuronide and kaempferol-3-O-?-D-glucuronide and their glucosides, for preventing diseases of the heart. Red vine leaf extract has proved a particularly advantageous source of the flavonoid compounds in question.

Abstract: The invention relates to a method for protecting transplants, as well as organs and individual blood vessels, against inflammatory reactions that are caused by the operative procedure linked to the explantation and storage until transplantation. This can be achieved by treating the transplant via intra arterial application of flavonol compounds, in particular with quercetin glucuronide and/or kaempferol glucuronide.

Abstract: The present invention relates to the use of flavonoid compounds, particularly quercetin-3-O-?-D-glucuronide and kaempferol-3-O-?-D-glucuronide and their glucosides, for preventing diseases of the heart. Red vine leaf extract has proved a particularly advantageous source of the flavonoid compounds in question.

Abstract: A perfusion liquid is suctioned from a perfusion liquid reservoir (16) through the automatic two-way valve (4) by drawing a syringe (5) serving as the perfusion liquid displacement device. For this purpose, the connection from the syringe (5) to the cannula (14) is closed by the two-way valve (4). The two-way valve (4) closes the connection to the perfusion liquid reservoir due to the pressure increase during the insertion of the syringe plunger and opens the connection from the syringe (5) to the cannula (14). A pressure relief valve (7) is attached at the T piece (6), exposing a discharge line (8) in case a threshold pressure is exceeded. A check valve (11) is attached at the extension of the cannula (14), preventing the backflow from the cannula (14) into the remaining system.

Abstract: The invention relates to a method and an apparatus for the endothelium-preserving treatment of isolated hollow organs, especially biological vessels such as blood vessels and lymphatic vessels by means of endothelium-protective perfusates or incubation solutions containing at least 0.1 percent by weight of native albumin and a nutrient substrate, preferably L-glutamine. Also disclosed are the use of the endothelium-protective perfusates for preparing hollow organs or biological vessels as a transplant for the treatment of organ diseases or vascular diseases, the use thereof for repairing endothelial lesions in isolated hollow organs and/or biological vessels, and the use thereof for preserving organs and/or vessels.

Abstract: The invention relates to a method for centrifugal particle separation, particularly in the biological sector, wherein a sample (26) to be fractionated is introduced into a centrifuge vessel (1) via a cannula (5), the free end (51) of which extends to point (1') of a centrifuge vessel (1) which sharply tapers toward tip (1') to minimize the undesirable effects of the Coriolis force, with a gradient solution (7) having a density that increases continuously or step by step then being introduced via cannula (5), with particles from sample (26) migrating due to the action of equilibrium and/or sedimentation centrifugation into gradient solution (7), and with a pressure fluid being introduced into the interior of sealed centrifuge vessel (1) via an additional cannula (17) after a predetermined centrifugation period to expel gradient solution (7) containing the fractionated particles of sample (26) via cannula (5).

Abstract: Apparatus for contacting a biological cell system on a substrate with a pulsating flow of perfusion fluid has a two-part housing for a combined holding and tensioning device which is removably inserted into an internal space to define with the housing and with the substrate therein two separate chambers. The housing has inlets and outlets for the two chambers and inlet and outlet valves which are operated in response to a reduction or increase of the volume of the respective chambers by elastic walls of the housing. The walls are oscillatable by cams and cam followers and the valves at the inlets open when the valves at the respective outlets are closed and vice versa. The characteristics of perfusion fluid prior to entry into and upon evacuation from the chambers are monitored to thus ascertain the nature of processes which take place at the cells on the substrate.

Abstract: A process for the pure preparation of particles, biological cell systems, colloids and the like, in which liquid density gradients are produced in a rotating centrifuge container (1), and in which the particles and the like are introduced into the centrifuge container (1), individual bands being produced in such a manner that the bands containing the heavier particles are more remote from the axis of rotation than are the bands containing the lighter particles. The density gradients are constructed by the introduction of fractions of different density (a-f) via a cannula (18) which extends into the centrifuge container (1) to just before a tip (19) of the latter, the lightest fraction (a) being introduced first and the heaviest fraction (f) being introduced last.

Abstract: A method and apparatus are disclosed for growing a confluent layer of cells on the surface of a substrate which is held by a holding and positioning device so as to be in the form of a taut, planar substrate having upper and lower exposed surfaces. The device is arranged in a nutrient-containing receptacle which also contains a plate material having a solid upper planar surface extending above the plane of the bottom of the receptacle. The lower layer of the receptacle is in contact with the upper surface of the plate material and cells disposed on the upper surface of the substrate are found to anchor to the substrate and grow to a confluent layer.

Abstract: Improved process for cultivation of matrix-bound biologic cell systems on microcarrier particles within a replenishable nutrient medium including the step of providing controlled three dimensional displacement of a culture vessel and its contents to effect uniform cell exposure to available nutrient material.

Abstract: Improved apparatus for cultivation of matrix-bound biologic cell systems on microcarrier particles within a replenishable nutrient medium providing controlled three dimensional displacement of a culture vessel and its contents to effect uniform cell exposure to available nutrient material.