Abstract: Methods and devices for detecting a low concentration analyte in a sample are provided herein. The methods include flowing a sample through a porous membrane coated with a capture matrix to capture the low concentration analyte. The methods also can include detecting the captured analyte, such as by performing in-situ amplification of the analyte.

Abstract: The presently-disclosed subject matter generally relates to methods, systems, compositions, and kits for the rapid, isothermal amplification of nucleic acids. The chemistry of the presently-disclosed amplification technique is isothermal, can be adapted to respond to a broad range of input target molecules, and results in a novel, biphasic reporter oligonucleotide amplification scheme with a high-gain second phase “burst” demonstrating a non-linear amplification rate (i.e., cooperative Hill kinetics). The switch-like amplification technique acts decisively to a true signal while filtering out noise, thus eliminating high levels of non-specific background amplification and false-positives.

Abstract: Disclosed herein is a diffusion-limiting reactor having a first element and a closure element, said reactor having at least two interconnected reservoirs said interconnection being by non-impinging microchannel, and at least one said reservoir and said microchannel being magnetic accessible. Further disclosed is a method of sample separation.