Abstract: A method for producing human-like glycoproteins by expressing a Class 2 ?-mannosidase having a substrate specificity for Man?1,3 and Man?1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

Abstract: A method for producing human-like glycoproteins by expressing a Class 2 ?-mannosidase having a substrate specificity for Man?1,3 and Man?1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

Abstract: A method for producing human-like glycoproteins by expressing a Class 2 ?-mannosidase having a substrate specificity for Man?1,3 and Man?1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

Abstract: Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a ?-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a ?-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable ?-mannosidase resistant ?-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.

Abstract: The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.

Abstract: Methods and apparatuses are described for an Intellectual Property (IP) Generator for estimating timing, area, and power characteristics of an electronic system design. The IP Generator receives a user-supplied file having data describing a configuration of an IP design having multiple levels of hierarchy. The IP Generator also receives user-supplied technology parameters and data-flow information. The IP generator correlates estimated timing, area, and power characteristics for each IP sub component based on the user supplied technology parameters, data-flow information and configuration parameters. The IP generator reports the timing, area, and power estimates to a user via a graphic user interface prior to a transformation of a Register Transfer Level (RTL) design into the gate-level circuit design.

Abstract: A riser kit for a grease interceptor having upwardly extending walls, an open top and a cover to close the open top. The riser kit comprises at least one collar extension having, a lower edge for engaging an upper edge of the upwardly extending walls of the grease interceptor, a middle portion forming an upward wall extension for the grease interceptor, and, a top edge sized and shaped to engage the cover to permit the cover to be secured to the extended grease interceptor. The riser kit also has a means to secure the collar extension to the grease interceptor. Also disclosed is a latch assembly for securing a cover onto a container, the latch assembly comprising a catch member secured to the container transverse to a container wall at an upper end thereof, a latch member pivotally mounted to the cover adjacent an edge thereof and positioned to register with the catch member.

Abstract: Methods and apparatuses are described for an Intellectual Property (IP) Generator for estimating timing, area, and power characteristics of an electronic system design. The IP Generator receives a user-supplied file having data describing a configuration of an IP design having multiple levels of hierarchy. The IP Generator also receives user-supplied technology parameters and data-flow information. The IP generator correlates estimated timing, area, and power characteristics for each IP sub component based on the user supplied technology parameters, data-flow information and configuration parameters. The IP generator reports the timing, area, and power estimates to a user via a graphic user interface prior to a transformation of a Register Transfer Level (RTL) design into the gate-level circuit design.

Abstract: A passive wireless sensor is disclosed. The sensor has at least a measurand sensitive member and an electromagnetically resonant member positioned proximate to each other. The resonant member comprises a preselected resonance frequency, such that it scatters at least a portion of an interrogating signal as a scattered signal proximate to its resonance frequency, and the measurand sensitive member alters the scattered signal as a function of the measurand to change the shape of the scattered signal. The reactive field of the sensor is kept within the sensor to minimize environment interference and to maximize its signal strength. Almost bond-free packaging mitigates problems with delamination or internal stresses due to differing coefficients of thermal expansion.

Abstract: Methods and apparatuses are described for an Intellectual Property (IP) Generator for estimating timing, area, and power characteristics of an electronic system design. The IP Generator receives a user-supplied file having data describing a configuration of an IP design having multiple levels of hierarchy. The IP Generator also receives user-supplied technology parameters and data-flow information. The IP generator correlates estimated timing, area, and power characteristics for each IP sub component based on the user supplied technology parameters, data-flow information and configuration parameters. The IP generator reports the timing, area, and power estimates to a user via a graphic user interface prior to a transformation of a Register Transfer Level (RTL) design into the gate-level circuit design.

Abstract: A method for producing human-like glycoproteins by expressing a Class 2 ?-mannosidase having a substrate specificity for Man?1,3 and Man?1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

Abstract: A method for producing human-like glycoproteins by expressing a Class 2 ?-mannosidase having a substrate specificity for Man?1,3 and Man?1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

Abstract: Methods are disclosed for genetically engineering host cells that lack an endogenous pathway for fucosylating N-glycans of glycoproteins to be able to produce glycoproteins with fucosylated N-glycans.

Abstract: A method for producing human-like glycoproteins by expressing a Class 2 ?-mannosidase having a substrate specificity for Man?1,3 and Man?1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.

Abstract: The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.

Abstract: A riser kit for a grease interceptor having upwardly extending walls, an open top and a cover to close the open top. The riser kit comprises at least one collar extension having, a lower edge for engaging an upper edge of the upwardly extending walls of the grease interceptor, a middle portion forming an upward wall extension for the grease interceptor, and, a top edge sized and shaped to engage the cover to permit the cover to be secured to the extended grease interceptor. The riser kit also has a means to secure the collar extension to the grease interceptor. Also disclosed is a latch assembly for securing a cover onto a container, the latch assembly comprising a catch member secured to the container transverse to a container wall at an upper end thereof, a latch member pivotally mounted to the cover adjacent an edge thereof and positioned to register with the catch member.

Abstract: The present invention provides methods for generating CMP-sialic acid in a non-human host which lacks endogenous CMP-Sialic by providing the host with enzymes involved in CMP-sialic acid synthesis from a bacterial, mammalian or hybrid CMP-sialic acid biosynthetic pathway. Novel fungal hosts expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided.

Abstract: The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.

Abstract: Methods and apparatuses are described for an Intellectual Property (IP) Generator for estimating timing, area and power constraints in an electronic design system. The IP Generator receives a user-supplied file having data describing a configuration of an intellectual property (IP) design, the data includes one or more configuration parameters. The IP Generator further enables a transformation of the user-supplied file into a register transfer level design description. Next, the IP Generator receives user-supplied technology parameters and data-flow information. The technology parameters describe a configuration of the IP design. Next, the IP Generator executes a timing module based on the configuration of the IP design as well as executes a timing model for each hierarchical level in the IP design. The timing model predicts timing paths of a final logic circuit.

Abstract: Methods and apparatuses are described for an Intellectual Property (IP) Generator for estimating timing, area, and power characteristics of an electronic system design. The IP Generator receives a user-supplied file having data describing a configuration of an IP design having multiple levels of hierarchy. The IP Generator also receives user-supplied technology parameters and data-flow information. The IP generator correlates estimated timing, area, and power characteristics for each IP sub component based on the user supplied technology parameters, data-flow information and configuration parameters. The IP generator reports the timing, area, and power estimates to a user via a graphic user interface prior to a transformation of a Register Transfer Level (RTL) design into the gate-level circuit design.