Abstract: The present invention relates to novel expression systems and methods for preparing samples for 3D structure determination of a protein based on a protein expression vector, E. coli host, and specific growth media.

Abstract: A method for reducing particle size of a particulate comprising feeding a feed material to a grinding mill having a power of at least 500 kW, the mill having a specific power draw of at least 50 kW per cubic meter of grinding volume of the mill and the grinding mill including a grinding media comprising particulate material having a specific gravity of not less than 2.4 tons/m3 and a particle size falling in the range of from about 0.8 to 8 mm, grinding the feed material in the grinding mill and removing a product from the grinding mill, the product having a particle size range such that D80 of the product is at least about 20 microns.

Abstract: A system includes an internal combustion engine having an air intake and a pressurizing device disposed in the air intake. The pressurizing device is a turbocharger or a supercharger. A gaseous fuel supply is fluidly coupled to the air intake at a position upstream of the pressurizing device through a first fluid conduit and fluidly coupled to the air intake at a position downstream of the pressurizing device through a second fluid conduit. A valve is disposed in the second fluid conduit and includes an electronic control input. The valve responds to an electronic signal at the electronic control input. The system includes an engine start indicating device electronically coupled to the electronic control input, where the engine start indicating device provides the electronic signal to open the valve in response to the engine being in a starting operating condition.

Abstract: System and method for removing threads from discussion groups based on ratings of the individual posts within the thread. If a user takes the time to rate a post positively, then the post (and the thread) is considered to be useful to the entire community and should not be deleted. To rate the post, a window is displayed to the user whenever the post is open and queries the user whether the post is useful. A positive rating resets a delete date associated with the post. If there is not positive rating of a post, the thread may be considered useful if the originator of a post marks the post as being answered, in which case delete clock is reset. If however, none of the posts within the thread are rated positively, then the thread is considered not be useful and deleted.

Abstract: A respiratory suction catheter assembly includes a manifold supporting a rotatable swivel member for engagement with an endotracheal tube, and a seal support body disposed on a boss of the manifold opposite the part supporting the swivel for supporting a catheter tube seal and a porous filter for filtering air flowing into and out of a flexible elongated sheath enclosing the catheter tube. The sheath includes opposed tapered ends secured to the seal support body and to a boss on a suction control valve housing. The valve housing supports a tapered plug closure member engageable with a resilient actuator and a slide-lock member. Certain components, including the sheath, the seal support body, the filter, the catheter tube seal and the catheter tube may be treated with an anti-microbial agent.

Abstract: According to one exemplary embodiment of the present invention, a center-pull bottom feed dispenser includes a housing defining a compartment and including a center-pull bottom feed arrangement for dispensing a sheet material that is in the form of a roll of the sheet material. The sheet material can be a pre-moistened airlaid web. The dispenser also includes a tray that supports the airlaid web roll and includes an outlet opening through which the airlaid web is fed to be accessible to a user. The tray includes a moisture retaining feature that directs fluid from the pre-moistened air-laid web away from the outlet opening and toward an outer peripheral edge of the roll.

Abstract: According to one exemplary embodiment of the present invention, a center-pull bottom feed dispenser includes a housing defining a compartment and including a center-pull bottom feed arrangement for dispensing a sheet material that is in the form of a roll of the sheet material. The sheet material can be a pre-moistened airlaid web. The dispenser also includes a tray that supports the airlaid web roll and includes an outlet opening through which the airlaid web is fed to be accessible to a user. The tray includes a moisture retaining feature that directs fluid from the pre-moistened air-laid web away from the outlet opening and toward an outer peripheral edge of the roll.

Abstract: A hybrid gas compressor has at least one rotor and shroud which define a compressor gas path extending from an inlet to an outlet. The compressor includes at least two compression stages and one diffusion stage between the inlet and outlet, the compression stages including respective circumferential arrays of blades extending from the rotor and the diffusion stage including a circumferential array of vanes between the compression stages. Blade aerodynamic loadings may be controlled, particularly in the last stage, to provide desired compression characteristics across the compressor. A bleed outlet is optionally located between the inlet and outlet for bleeding from the gas path.

Abstract: A suction catheter assembly, particularly adapted for neonatal use includes a distal manifold body including a rotatable connector for connection to an endotracheal tube, a ventilating port member and an ancillary catheter receiving port member. The manifold body is rotatably joined to a catheter tube seal housing. The seal housing includes an irrigation port member. A catheter tube wiper seal and a filter member are disposed in the seal housing which is joined to a suction control valve assembly by an elongated flexible sheath defining an enclosed space through which the catheter tube extends between the valve assembly and the manifold. A resilient closure member actuator and slide lock member are engageable with a closure member of the valve assembly.

Abstract: The present invention relates to methods for the production of isoprene by the direct conversion of atmospheric carbon dioxide using metabolically engineered genetically engineered photosynthetic microorganisms. The present invention also relates to genetically engineered photosynthetic microorganisms, such as cyanobacteria, that are capable of producing isoprene from CO2.

Abstract: In one embodiment, an access device is inserted through an incision in skin of a patient. The access device is expanded from a first configuration to a second configuration, the second configuration having an enlarged cross-sectional area at a distal portion of said access device such that the distal portion extends across at least a portion of the interbody space. A prosthetic spinal disc implant is then delivered through the access device.

Abstract: A method for reducing particle size of a particulate comprising feeding a feed material to a grinding mill having a power of at least 500 kW, the mill having a specific power draw of at least 50 kW per cubic metre of grinding volume of the mill and the grinding mill including a grinding media comprising particulate material having a specific gravity of not less than 2.4 tonnes/m3 and a particle size falling in the range of from about 0.8 to 8 mm, grinding the feed material in the grinding mill and removing a product from the grinding mill, the product having a particle size range such that D80 of the product is at least about 20 microns.

Abstract: An event handling system to schedule and translate semantic deductions form Intelligent Agents and sensors into events capable of being made observable by a Recipient system such as monitor that provides a particular view of virtual objects and events is disclosed. The event handling system also encapsulates the system's notion of time. In fact, a human observer can shift the system along the temporal axis (up to the present) in order to replay events, or undertake analyses as a result of speeded-up or slowed-down notions of system time. The event handling system receives events from Clients/Sources via connections through the event handling system Input Portals, and uses Shared Memory as its form of inter-process communication with the Monitors. The event handling system makes events available for a recipient observation sub-system to read and provide their particular view. There can be many Clients and Recipient systems connected to the event handling system at the same time.

Abstract: An ink jet cartridge comprising a housing having a print head portion including a nozzle plate having opposed pairs of side edges, rows of nozzles and at least one row of slots between said rows of nozzles and which plate is initially attached to the head portion by beads of an adhesive material overlying the edges of one pair of the opposed pairs of side edges is prepared for renovation by applying a reinforcing bead of an adhesive material to overlie at least one of the other pair of the opposed pairs of side edges and the at least one row of slots. The beads may be a single bead or segmented and are applied by robotic glue applying apparatus.

Abstract: Systems, devices, and methods suitable for use with procedures performed at least partially percutaneously are provided. In some procedures, two or more access devices for providing access to adjacent surgical locations within a patient are used. Certain embodiments of the access device comprise an elongate body having a distal end with one or more cutouts. The cutouts on adjacent access devices are generally aligned with each other to permit passage of a portion of a fixation element from one access device to the other access device. A fastener with an elongated removable head may be delivered to the surgical site through the access device. After a distal end of the fastener is secured to the surgical site, a portion of the elongated housing is detached from the remainder of the fastener and removed from the patient.

Abstract: Systems, devices, and methods suitable for use with procedures performed at least partially percutaneously are provided. In some procedures, two or more access devices for providing access to adjacent surgical locations within a patient are used. Certain embodiments of the access device comprise an elongate body having a distal end with one or more cutouts. The cutouts on adjacent access devices are generally aligned with each other to permit passage of a portion of a fixation element from one access device to the other access device. A fastener with an elongated removable head may be delivered to the surgical site through the access device. After a distal end of the fastener is secured to the surgical site, a portion of the elongated housing is detached from the remainder of the fastener and removed from the patient.

Abstract: Systems, devices, and methods suitable for use with procedures performed at least partially percutaneously are provided. In some procedures, two or more access devices for providing access to adjacent surgical locations within a patient are used. Certain embodiments of the access device comprise an elongate body having a distal end with one or more cutouts. The cutouts on adjacent access devices are generally aligned with each other to permit passage of a portion of a fixation element from one access device to the other access device. A fastener with an elongated removable head may be delivered to the surgical site through the access device. After a distal end of the fastener is secured to the surgical site, a portion of the elongated housing is detached from the remainder of the fastener and removed from the patient.

Abstract: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising disruptions in PRO224, PRO9783, PRO1108, PRO34000, PRO240, PRO943, hu A33, PRO230, PRO178, PRO1199, PRO4333, PRO1336, PRO19598, PRO1083, hu TRPM2 or PRO1801 genes. Such in vivo studies and characterizations may provide valuable identification and discovery of therapeutics and/or treatments useful in the prevention, amelioration or correction of diseases or dysfunctions associated with gene disruptions such as neurological disorders; cardiovascular, endothelial or angiogenic disorders; eye abnormalities; immunological disorders; oncological disorders; bone metabolic abnormalities or disorders; lipid metabolic disorders; or developmental abnormalities.

Abstract: A tissue dispenser is provided by which a user may access one or more rolls of tissue. The dispenser generally includes a base and a cover. The dispenser includes at least one spindle, and preferably left and right spindles, with each spindle being supported by and extending from the base. The dispenser also has a plurality of sleeves interchangeably disposed on the shafts of the spindles. Each sleeve has an inner diameter dimensioned to receive a corresponding spindle, and an outer diameter. A plurality of fins is radially disposed on the outer diameter of the sleeve to circumferentially support the inner diameter of a core of a tissue roll. Different sleeves having different outer diameters to accommodate tissue rolls having different core sizes are provided. The dispenser may also include a door assembly configured to selectively cover a tissue roll on either the left or the right side of the base. A plurality of snap fittings configured to releasably attach to the door assembly may be provided.

Abstract: A suction catheter assembly, particularly adapted for neonatal use includes a distal manifold body including a rotatable connector for connection to an endotracheal tube, a ventilating port member and an ancillary catheter receiving port member. The manifold body is rotatably joined to a catheter tube seal housing. The seal housing includes an irrigation port member. A catheter tube wiper seal and a filter member are disposed in the seal housing which is joined to a suction control valve assembly by an elongated flexible sheath defining an enclosed space through which the catheter tube extends between the valve assembly and the manifold. A resilient closure member actuator and slide lock member are engageable with a closure member of the valve assembly.