Abstract: A method of predicting risk of an aggressive or a recurrent prostate cancer in an individual, the method comprising a step of assaying a biological sample from the individual for expression of at least two genes, or proteins encoded by said genes, selected from TTC21B, MCM3, MTUS1, FOXM1, CUL4A, GPC1, PCNA, IRF7, BRIP1, ULKI, and SPAG5, wherein when a combination of a normalised expression value of the at least two genes, or proteins encoded by said genes, is modified relative to a combination of a normalised expression reference value of the at least two genes, or proteins encoded by said, in a biological sample from a subject with non-aggressive or non-recurrent prostate cancer, the individual is predicted to have an increased risk of having an aggressive or a recurrent prostate cancer.

Abstract: The invention provides a flow system, a subsea valve, and a method of use in a subsea pipeline filling, flooding or pigging operation. The flow system comprises a subsea valve comprising a valve inlet and a valve outlet configured to be coupled to a subsea pipeline. A pump comprises a pump inlet connected to a fluid source and a pump outlet connected to the valve inlet. The pump is operable to pump fluid from the fluid source and into the subsea pipeline via the subsea valve. The subsea valve comprises a movable valve member and a biasing mechanism, by which the valve member is urged by a biasing force towards a closed position that prevents flow of fluid through the valve and into the subsea pipeline. The valve member is operable to be moved to an open position on activation of the pump to provide a pressure increase at the valve inlet sufficient to overcome the biasing force. In use, opposing sides of the valve member are exposed to ambient subsea pressure such that the subsea valve is pressure balanced.

Abstract: The invention provides a flow system, a subsea valve (100), and a method of use in a subsea pipeline filling, flooding or pigging operation. The flow system comprises a subsea valve (100) comprising a valve inlet and a valve outlet configured to be coupled to a subsea pipeline (13). A pump (112) comprises a pump inlet connected to a fluid source and a pump outlet connected to the valve inlet. The pump is operable to pump fluid from the fluid source and into the subsea pipeline via the subsea valve. The subsea valve comprises a movable valve member and a biasing mechanism, by which the valve member is urged by a biasing force towards a closed position that prevents flow of fluid through the valve and into the subsea pipeline. The valve member is operable to be moved to an open position on activation of the pump to provide a pressure increase at the valve inlet sufficient to overcome the biasing force.