Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: A method, a device, and a non-transitory storage medium provide a vessel detention prevention and risk assessment service. The service may determine whether a vessel has a detention risk upon arrival at a next destination. The service may initiate actions to be taken to minimize or prevent the detention risk before arrival. The service may also calculate an overall risk of the vessel based on registry data, port state control data, and/or class society data.

Abstract: The invention relates generally to apparatus and devices for neuronavigation. A non-invasive dental based fiducial array is disclosed. The fiducial array has a base support member, one or more removable and repositionable marker bases supported by the base support member and a stopper spaced from and supported by the base support member that engages a part of the head of a patient or an animal and cooperates with the base support member to immobilize the fiducial array relative to a location of the head, such as maxillary teeth. An anchoring site is formed on the base support member to accept a custom made dental impression to precisely position the base support member relative to the location of the head.