Patents by Inventor Stephen G. Sligar
Stephen G. Sligar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10900971Abstract: The present invention provides biomimetic sensor devices that utilize proteins—such G-protein coupled receptors—and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.Type: GrantFiled: November 16, 2016Date of Patent: January 26, 2021Assignees: The Trustees of the University of Pennsylvania, The Board of Trustees of the University of IllinoisInventors: Alan T. Johnson, Jr., Brett R. Goldsmith, Joseph J. Mitala, Jr., Bohdana M. Discher, Stephen G. Sligar, Timothy H. Bayburt
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Publication number: 20170299602Abstract: The present invention provides biomimetic sensor devices that utilize proteins—such G-protein coupled receptors—and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.Type: ApplicationFiled: November 16, 2016Publication date: October 19, 2017Inventors: Alan T Johnson, Jr., Brett R Goldsmith, Joseph J Mitala, Jr., Bohdana Discher, Stephen G Sligar, Timothy H Bayburt
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Patent number: 9612240Abstract: The present invention provides biomimetic sensor devices that utilize proteins—such as G-protein coupled receptors—and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.Type: GrantFiled: June 29, 2011Date of Patent: April 4, 2017Assignee: The Trustees Of The University Of PennsylvaniaInventors: Alan T. Johnson, Jr., Brett R. Goldsmith, Joseph J. Mitala, Jr., Bohdana Discher, Stephen G. Sligar, Timothy H. Bayburt
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Publication number: 20150065363Abstract: The present invention provides biomimetic sensor devices that utilize proteins—such as G-protein coupled receptors—and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.Type: ApplicationFiled: June 29, 2011Publication date: March 5, 2015Applicant: the Trustees of The University of PennsylvaniaInventors: Alan T. Johnson, JR., Brett R. Goldsmith, Joseph J. Mitala, JR., Bohdana Discher, Stephen G. Sligar, Timothy H. Bayburt
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Patent number: 7691414Abstract: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.Type: GrantFiled: November 2, 2004Date of Patent: April 6, 2010Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen G. Sligar, Timothy H. Bayburt
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Patent number: 7662410Abstract: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.Type: GrantFiled: May 23, 2006Date of Patent: February 16, 2010Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen G. Sligar, Timothy H. Bayburt
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Patent number: 7622437Abstract: Tissue Factor (natural or recombinant truncated) can be incorporated into stable, soluble nanoscale particles so that activity is maintained. These particles can be used as a reagent in prothrombin clotting time assays or they can be used in therapeutic compositions for use in humans or animals. Therapeutic settings can include supplementation in the case of a genetic deficiency, uncontrolled bleeding, surgical incisions or seepage, thrombocytopenia, soft tissue trauma or other trauma, to effect tumor regression or to inhibit tumor growth.Type: GrantFiled: October 27, 2005Date of Patent: November 24, 2009Assignee: The Board of Trustees of the University of IllinoisInventors: James H. Morrissey, Vincent S. Pureza, Stephen G. Sligar
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Publication number: 20090257950Abstract: The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, both in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlations, structure determinations, bioseparations, and drug discovery.Type: ApplicationFiled: October 10, 2007Publication date: October 15, 2009Applicant: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOISInventors: Stephen G. Sligar, Timothy H. Bayburt, Mary A. Schuler, Natanya R. Civjan, Yelena V. Grinkova, Ilia G. Denisov, Stephen James Grimme
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Patent number: 7592008Abstract: The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlations, structure determinations, bioseparations, and drug discovery.Type: GrantFiled: January 11, 2005Date of Patent: September 22, 2009Assignee: The Board of Trustees of the University of Illinois, a body corporate and politic of the State of IllinoisInventors: Stephen G. Sligar, Timothy H. Bayburt, Mary A. Schuler, Natanya R. Civjan, Ylena V. Grinkova, Ilia G. Denisov, Stephen James Grimme
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Patent number: 7575763Abstract: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.Type: GrantFiled: May 23, 2006Date of Patent: August 18, 2009Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen G. Sligar, Timothy H. Bayburt
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Publication number: 20090047356Abstract: Tissue Factor (natural or recombinant truncated) can be incorporated into stable, soluble nanoscale particles so that activity is maintained. These particles can be used as a reagent in prothrombin clotting time assays or they can be used in therapeutic compositions for use in humans or animals. Therapeutic settings can include supplementation in the case of a genetic deficiency, uncontrolled bleeding, surgical incisions or seepage, thrombocytopenia, soft tissue trauma or other trauma, to effect tumor regression or to inhibit tumor growth.Type: ApplicationFiled: September 16, 2008Publication date: February 19, 2009Applicant: The Board of Trustees of the University of IllinoisInventors: James H. Morrissey, Vincent S. Pureza, Stephen G. Sligar
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Patent number: 7083958Abstract: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. The membrane scaffold proteins (MSP) of the present invention assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance.Type: GrantFiled: June 18, 2003Date of Patent: August 1, 2006Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen G. Sligar, Timothy H. Bayburt, Mary A. Schuler, Natanya R Civjan, Yelena V. Grinkova, Ilia G. Denisov
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Patent number: 7048949Abstract: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.Type: GrantFiled: November 20, 2001Date of Patent: May 23, 2006Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen G. Sligar, Timothy H. Bayburt
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Publication number: 20040053384Abstract: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. The membrane scaffold proteins (MSP) of the present invention assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance.Type: ApplicationFiled: June 18, 2003Publication date: March 18, 2004Inventors: Stephen G. Sligar, Timothy H. Bayburt, Mary A. Schuler, Natanya R. Civjan, Yelena V. Grinkova, Ilia G. Denisov
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Patent number: 6235500Abstract: Described are preferred oxygen-binding heme proteins which include at least one hemoglobin molecule incorporating at least one circularly permuted globin, especially an alpha globin. More preferred heme proteins of the invention include high molecular weight hemoglobin multimers. Also described are polynucleotides encoding proteins of the invention, and vectors and host cells including the same.Type: GrantFiled: June 28, 1999Date of Patent: May 22, 2001Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen G. Sligar, Kevin Sanders
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Patent number: 5466589Abstract: The present invention is directed to coated substrates having a coating of biological macromolecules, preferably proteins, which are capable of being immobilized on a substrate surface and have a marker. These proteins usually are mutant proteins obtained by mutagenesis of the gene encoding a random positioning protein. When a mutant protein molecule is immobilized on the substrate, the marker of the mutant protein molecule is in a select spatial relationship with both the substrate and the markers of adjacent protein molecules. A substrate coated with an oriented layer of the mutant proteins exhibits improved or different properties when compared to a substrate having a randomly positioned layer of proteins thereon.Type: GrantFiled: September 11, 1992Date of Patent: November 14, 1995Assignee: Biotechnology Research & Development CorporationInventors: Jill M. Olinger, Stephen G. Sligar, Mark A. McLean, Paul W. Bohn, Patrick Stayton