Abstract: The present invention relates to a method of preventing, reducing, or treating cancer in a subject, comprising administering a therapeutically effective amount of or a pharmaceutically acceptable salt, free base, hydrate, complex, or chelate (including metal chelates, such as iron, zinc and others) thereof to the subject, wherein the subject has a mutation in a DNA repair gene.

Abstract: The present invention relates to a method of preventing, reducing, or treating cancer in a subject, comprising administering a therapeutically effective amount of or a pharmaceutically acceptable salt, free base, hydrate, complex, or chelate (including metal chelates, such as iron, zinc and others) thereof to the subject, wherein the subject has a mutation in a DNA repair gene.

Abstract: A method is disclosed for producing a protein which expresses bioluminescence activity which involves combining two polydeoxyribonucleotides, one containing a continuous sequence of codons encoding a polypeptide which comprises a single covalently bonded molecular structure and which catalyzes the oxidation of insect luciferin to yield light and the other which causes DNA transcription, and obtaining the polypeptide by transcription and subsequent translation. The insect luciferin is derived from bioluminescent insect, preferably Diptera and Coleoptera (fireflies and beetles). Hybrid proteins are similarly formed by inclusion of an additional polydeoxyribonucleotide encoding for a second polypeptide such that their respective polypeptide-encoding reading frames form a continuous reading frame.

Abstract: A method is disclosed for producing a protein which expresses bioluminescence activity which involves combining two polydeoxyribonucleotides, one containing a continuous sequence of codons encoding a polypeptide which comprises a single covalently bonded molecular structure and which catalyzes the oxidation of insect luciferin to yield light and the other which causes DNA transcription, and obtaining the polypeptide by transcription and subsequent translation. The insect luciferin is derived from bioluminescent insect, preferably Diptera and Coleoptera (fireflies and beetles). Hybrid proteins are similarly formed by inclusion of an additional polydeoxyribonucleotide encoding for a second polypeptide such that their respective polypeptide-encoding reading frames form a continuous reading frame.

Abstract: Novel methods and compositions are provided for preparing vectors for the introduction of DNA into plant cells for transcription and expression of the DNA. Particularly, cauliflower mosaic virus DNA is inserted into a bacterial cloning vehicle to provide a recombinant plasmid for cloning in a microorganism. The resulting cloned plasmid is genetically manipulated to introduce exogenous or heterologous DNA. Conveniently, linkers can be inserted which provide for a unique restriction site for insertion of exogenous or heterologous DNA. At each stage the modified plasmid may be cloned to provide for relatively large amounts of material for modification and isolation. Besides insertions, deletions may be made, removing non-essential portions of the virus. After completion of the viral modifications, the CaMV is excised from the hybrid plasmid by restriction enzyme cleavage and may be used for systemic infection of plants.