Abstract: The present invention relates to a contact cleaning apparatus (100) including an elastomeric roller (120), rotatably mounted within a contact cleaning apparatus such that an outer surface (123) of said elastomeric roller contactingly engages a substrate surface (112) during use. There is also provided an apparatus for evaluating said outer surface of said elastomeric roller, including an electromagnetic radiation source (140), configured to selectively emit electromagnetic radiation of a predetermined electromagnetic spectrum onto at least one predetermined region (125) of said outer surface, a detector (160), configured to receive at least a first reflection of said electromagnetic radiation from said at least one predetermined region, and a controller (270) operably coupled to said detector and adapted to determine at least one characteristic parameter of at least said first reflection.

Abstract: Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Abstract: Methods, apparatus, systems, computing devices, computing entities, and/or the like for a hybrid question-answering (QA) application are provided. The hybrid QA application includes both a retrieval QA application and a deep QA application, and uses feedback from the retrieval QA application to improve the deep QA application.

Abstract: A pathway identification system includes processor and memory hardware. The memory hardware stores a pathway database including pathways corresponding to at least one pathway parameter, a framework database including a set of framework transformation models, and instructions. The instructions include, in response to receiving a first pathway parameter corresponding to a user, identifying a subset of pathways of the pathways corresponding to the first pathway parameter. The instructions include obtaining a first set of framework transformation models from the framework database, determining a pathway value for each pathway of the subset of pathways using the first set of framework transformation models, and selecting at least one pathway of the subset of pathways based on the corresponding pathway value. The instructions include transmitting information encoding the at least one pathway to the operator device and transforming a user interface of the operator device to display the at least one pathway.

Abstract: A pathway identification system includes processor and memory hardware. The memory hardware stores a pathway database including pathways corresponding to at least one pathway parameter, a framework database including a set of framework transformation models, and instructions. The instructions include, in response to receiving a first pathway parameter corresponding to a user, identifying a subset of pathways of the pathways corresponding to the first pathway parameter. The instructions include obtaining a first set of framework transformation models from the framework database, determining a pathway value for each pathway of the subset of pathways using the first set of framework transformation models, and selecting at least one pathway of the subset of pathways based on the corresponding pathway value. The instructions include transmitting information encoding the at least one pathway to the operator device and transforming a user interface of the operator device to display the at least one pathway.

Abstract: A pathway identification system includes processor and memory hardware. The memory hardware stores a pathway database including pathways corresponding to at least one pathway parameter, a framework database including a set of framework transformation models, and instructions. The instructions include, in response to receiving a first pathway parameter corresponding to a user, identifying a subset of pathways of the pathways corresponding to the first pathway parameter. The instructions include obtaining a first set of framework transformation models from the framework database, determining a pathway value for each pathway of the subset of pathways using the first set of framework transformation models, and selecting at least one pathway of the subset of pathways based on the corresponding pathway value. The instructions include transmitting information encoding the at least one pathway to the operator device and transforming a user interface of the operator device to display the at least one pathway.

Abstract: A pathway identification system includes processor and memory hardware. The memory hardware stores a pathway database including pathways corresponding to at least one pathway parameter, a framework database including a set of framework transformation models, and instructions. The instructions include, in response to receiving a first pathway parameter corresponding to a user, identifying a subset of pathways of the pathways corresponding to the first pathway parameter. The instructions include obtaining a first set of framework transformation models from the framework database, determining a pathway value for each pathway of the subset of pathways using the first set of framework transformation models, and selecting at least one pathway of the subset of pathways based on the corresponding pathway value. The instructions include transmitting information encoding the at least one pathway to the operator device and transforming a user interface of the operator device to display the at least one pathway.

Abstract: A trained predictive server is provided for determining that a diagnosis and treatment plan is inaccurate. The trained predictive server includes a processor configured to receive a set of prior authorization (PA) data associated with a medical claim for a patient, and determine that the set of PA data indicates that the medical claim is associated with a qualifying critical illness. The processor is further configured to extract component data from the set of PA data, and apply the extracted component data to a trained predictive model associated with the qualifying critical illness to determine whether the medical claim is associated with an inaccurate diagnosis and treatment plan. Upon determining that the medical claim is associated with an inaccurate diagnosis and treatment plan, the processor is configured to generate a request for a consulting review of the diagnosis and treatment plan using the set of PA data.

Abstract: Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a ?-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a ?-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable ?-mannosidase resistant ?-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.

Abstract: The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.

Abstract: Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.

Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g.

Abstract: Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a ?-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a ?-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable ?-mannosidase resistant ?-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.

Abstract: The present invention provides, in part, promoters for recombinant expression of polypeptides in host cells such as Pichia pastoris as well as methods of use thereof.

Abstract: Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.