Patents by Inventor Stephen J. Brewer

Stephen J. Brewer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fused polypeptides and methods for their detection
    Patent number: 4880911
    Abstract: Fused-Polypeptides comprising a polypeptide product and a covalently bonded charged amino acid polymer are disclosed. Methods of detecting the presence of the charged amino acid polymer are used to quantitate the presence of the fused-polypeptides either in crude or purified solutions. The charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide product. Examples using polyarginine and carboxypeptidase B to produce urogastrone and gamma interferon are included.
    Type: Grant
    Filed: May 9, 1985
    Date of Patent: November 14, 1989
    Assignee: G. D. Searle & Co.
    Inventors: Stephen J. Brewer, Helmut M. Sassenfeld
  • Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
    Patent number: 4532207
    Abstract: A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminants by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.
    Type: Grant
    Filed: March 3, 1983
    Date of Patent: July 30, 1985
    Assignee: G. D. Searle & Co.
    Inventors: Stephen J. Brewer, Helmut M. Sassenfeld
  • O-Transcarbamoylase
    Patent number: 4164447
    Abstract: A novel O-transcarbamoylase enzyme and its use in preparing a variety of 3-carbamoyloxymethyl cephalosporins from 3-hydroxymethyl cephalosporins and carbamoyl compounds are described.
    Type: Grant
    Filed: November 22, 1977
    Date of Patent: August 14, 1979
    Assignee: Glaxo Laboratories Limited
    Inventors: Ian D. Fleming, Michael K. Turner, Stephen J. Brewer
  • Method for producing cephalosporins
    Patent number: 4075061
    Abstract: A novel O-transcarbamoylase enzyme and its use in preparing a variety of 3-carbamoyloxymethyl cephalosporins from 3-hydroxymethyl cephalosporins and carbamoyl compounds are described.
    Type: Grant
    Filed: February 15, 1977
    Date of Patent: February 21, 1978
    Assignee: Glaxo Laboratories Limited
    Inventors: Ian D. Fleming, Michael K. Turner, Stephen J. Brewer