Patents by Inventor Stephen J. Garger
Stephen J. Garger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7413889Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.Type: GrantFiled: February 4, 2002Date of Patent: August 19, 2008Assignee: Kentucky Bioprocessing, LLCInventors: Gregory P. Pogue, John A. Lindbo, Michael J. McCulloch, Jonathan E. Lawrence, Cynthia S. Gross, Stephen J. Garger
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Patent number: 7270825Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.Type: GrantFiled: July 12, 2002Date of Patent: September 18, 2007Assignee: Large Scale Biology CorporationInventors: Gregory P. Pogue, John A. Lindbo, Michael J. McCulloch, Jonathan E. Lawrence, Cynthia S. Gross, Stephen J. Garger
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Patent number: 7192740Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: GrantFiled: October 24, 2002Date of Patent: March 20, 2007Assignee: Large Scale Biology CorporationInventors: Jonathan Donson, William O. Dawson, George L. Grantham, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Jr., Laurence K. Grill
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Patent number: 7048211Abstract: A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: February 18, 2004Date of Patent: May 23, 2006Assignee: Large Scale Biology CorporationInventors: Barry Bratcher, Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch
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Patent number: 7034128Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: October 29, 2004Date of Patent: April 25, 2006Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Patent number: 6906172Abstract: A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: October 3, 2001Date of Patent: June 14, 2005Assignee: Large Scale Biology CorporationInventors: Barry Bratcher, Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch
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Patent number: 6890748Abstract: The invention relates to ?-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.Type: GrantFiled: March 20, 2002Date of Patent: May 10, 2005Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, Thomas H. Turpen, Monto H. Kumagai
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Patent number: 6887696Abstract: The invention relates to ?-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human ? galactosidase nucleotide sequences.Type: GrantFiled: November 13, 2001Date of Patent: May 3, 2005Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, Thomas H. Turpen, Monto H. Kumagai
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Patent number: 6841659Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: August 1, 2003Date of Patent: January 11, 2005Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Publication number: 20040234516Abstract: The invention relates to &agr;-galactosidase truncated at the carboxy terminus and the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human a galactosidase nucleotide sequences.Type: ApplicationFiled: May 21, 2004Publication date: November 25, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Stephen J. Garger, Thomas H. Turpen, Monto H. Kumagai
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Publication number: 20040171813Abstract: The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art.Type: ApplicationFiled: April 20, 2004Publication date: September 2, 2004Inventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
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Publication number: 20040166026Abstract: A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: ApplicationFiled: February 18, 2004Publication date: August 26, 2004Inventors: Barry Bratcher, Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch
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Patent number: 6740740Abstract: The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a larger scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target protein/peptide from other components of the green juice by one or more cycles of centrifugation and/or resuspension, and finally purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: September 24, 2001Date of Patent: May 25, 2004Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
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Patent number: 6730306Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.Type: GrantFiled: March 8, 2000Date of Patent: May 4, 2004Assignee: Large Scale Biology CorporationInventors: Gregory P. Pogue, John A. Lindbo, Michael J. McCulloch, Jonathan E. Lawrence, Cynthia S. Gross, Stephen J. Garger
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Publication number: 20040047923Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: ApplicationFiled: August 1, 2003Publication date: March 11, 2004Inventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Publication number: 20040049025Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: ApplicationFiled: October 24, 2002Publication date: March 11, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Jon Donson, William O. Dawson, George L. Grantham, Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill
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Patent number: 6617435Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: April 8, 2002Date of Patent: September 9, 2003Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Publication number: 20030150019Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.Type: ApplicationFiled: October 24, 2002Publication date: August 7, 2003Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Thomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill, Jonathan Donson, William O. Dawson, George L. Granthan
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Publication number: 20030118596Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.Type: ApplicationFiled: April 24, 2002Publication date: June 26, 2003Inventors: Gregory P. Pogue, John A. Lindbo, Michael J. McCulloch, Jonathan E. Lawrence, Cynthia S. Gross, Stephen J. Garger
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Publication number: 20030108557Abstract: The present invention relates to foreign peptide sequences fused to the N-terminal of plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The foreign peptide sequences can be cleaved from the fusion proteins by proteolytic enzymes or chemical reagents. The foreign peptide sequences of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections, for use as a protein involved in hormonal activity, or for use as a protein involved in immunoregulatory activity.Type: ApplicationFiled: November 1, 2002Publication date: June 12, 2003Applicant: Large Scale Biology CorporationInventors: Stephen J. Garger, Cynthia Gross, John A. Lindbo, Gregory P. Pogue