Abstract: A mass spectrometry substrate includes an electrically conductive material providing an electrical conductivity that allows at least one of a first and a second surface of the substrate to be maintained at a desirable potential for ion extraction while ions are desorbed during ionization. A solid lattice material comprises a plurality of pores positioned in a plurality of layers that form a network of at least one continuous channel extending from a first surface of the substrate to a second surface of the substrate. Each of the plurality of pores are dimensioned and positioned in the plurality of layers so that a first group of substances are adsorbed or absorbed on the first surface and a second group of substances are adsorbed or absorbed on the second surface.

Abstract: A mass spectrometry substrate includes an electrically conductive material providing an electrical conductivity that allows at least one of a first and a second surface of the substrate to be maintained at a desirable potential for ion extraction while ions are desorbed during ionization. A solid lattice material comprises a plurality of pores positioned in a plurality of layers that form a network of at least one continuous channel extending from a first surface of the substrate to a second surface of the substrate. Each of the plurality of pores are dimensioned and positioned in the plurality of layers so that a first group of substances are adsorbed or absorbed on the first surface and a second group of substances are adsorbed or absorbed on the second surface.

Abstract: A mass spectrometry substrate for performing adsorption and biological processing includes a porous silica-based material comprising a chemical adsorption material that captures analyte(s) of interest. A biologically active material incorporated into the porous silica-based material performs biological processing on the analyte(s) of interest.

Abstract: In various aspects, provided are methods for identification of the presence of modified peptides using at least in part mass spectrometric signals from metastable ions that are formed in the field free region of the analyzer. For example, in various embodiments where the modified peptides are phosphopeptides, such metastable ions can appear approximately 95 u lower than the precursor ion m/z value from which they were formed when the peptide contains a phosphate group. In various embodiments, detection of such metastable ions in a MALDI-TOF mass spectrum can identify the modified peptide. In various aspects, provided are methods for identification of the presence of modified peptides using at least in part MS/MS data from two or more of modified peptides, de-modified peptides and non-modified peptides by comparing the sequence and/or site of modification information for two or more of these three forms of a peptide.

Abstract: The present invention is directed to improved systems and components for coupling separations technology with MALDI-TOF mass spectroscopy. Specifically, this invention provides new apparatus configurations and methods for interfacing liquid-phase separations with MALDI mass spectrometry. The present invention exploits and expands the use of the collimated hole structure (CHS) sample plate as an integral component of the interface.