Abstract: In accordance with the present invention there is provided a method for the separation of a triglycoalkaloid in which the triglycoside portion comprises ?-L-rhamnopyranosyl-(1?2gal)-O-?-D-glucopyranosyl-(1?3gal)-?-D-galactopyranose (or 6-deoxy-?-L-mannopyranosyl-(1?2)-O-[?-D-glucopyranosyl-(1?3)]-?-D-galactopyranoside) (‘an rhamnose-glucose-galactose triglycoalkaloid’) from a triglycoalkaloid in which the triglycoside portion is ?-L-rhamnopyranosyl-(1?2glu)-O-?-L-rhamnopyranosyl-(1?4glu)-?-D-glucopyranose (‘a rhamnose-rhamnose-glucose triglycoalkaloid’) in a mixture containing both, the method comprising the steps of: Combining the mixture with a portion of alcoholic solvent; Causing or allowing a substantial portion of the rhamnose-rhamnose-glucose triglycoalkaloid to dissolve, thereby generating an alcoholic solution substantially of the rhamnose-rhamnose-glucose triglycoalkaloid, and a solid substantially comprising the rhamnose-glucose-galactose triglycoalkaloid; and Separating the solid substantially com

Abstract: An isolated RBP with at least one of the following characteristics: a) a molecular weight of approximately 65-70 kDa and more preferably 66-69 kDa; b) a pl of greater than 10 or less than 3; c) a dissociation constant of approximately 1.5×10?6 when bound to the rhamnose moiety of solamargine; d) adapted to bind to a rhamnose affinity column prepared according to example 1 and under the conditions set out therein; e) adapted to be eluted from the column in example 1 with a 100 mM rhamnose solution; f) insoluble in aqueous solution; and g) soluble in highly denaturing buffers containing greater that approximately 2% surfactant.

Abstract: A composition comprising at least two glycoalkaloids of formula I: 1

Abstract: In accordance with the present invention there is provided a method for the separation of a triglycoalkaloid in which the triglycoside portion comprises &agr;-L-rhamnopyranosyl-(1→2gal)-O-&bgr;-D-glucopyranosyl-(1→3gal)-&bgr;-D-galactopyranose (or 6-deoxy-&agr;-L-mannopyranosyl-(1→2)-O-[&bgr;-D-glucopyranosyl-(1→3)]-&bgr;-D-galactopyranoside) (‘an rhamnose-glucose-galactose triglycoalkaloid’) from a triglycoalkaloid in which the triglycoside portion is &agr;-L-rhamnopyranosyl-(1→2glu)-O-&agr;-L-rhamnopyranosyl-(1→4glu)-&bgr;-D-glucopyranose (‘a rhamnose-rhamnose-glucose triglycoalkaloid’) in a mixture containing both, the method comprising the steps of:

Abstract: An isolated RBP with at least one of the following characteristics: