Abstract: An assay for a metalloprotease comprises (i) combining a test compound with a substrate, wherein the protease reacts with the substrate to form a product; and (ii) detecting presence of the metalloprotease by measuring the mass of the product.

Abstract: A tool holder (32) includes a rotary portion (36) securable in a spindle (20) of a machining center (10). A pumped flow of primary coolant (16) is delivered through spindle (20) and rotary portion (36). A pumped flow of secondary coolant (22) is delivered to a static connection (26) and through the static portion (34) of the tool holder. A tool guard assembly (42) secured to static portion (34) surrounds an installed superabrasive grinding wheel (66). It also includes flow paths (50, 52, 98; 62, 68, 82 84) and nozzles (70, 80, 86, 94) to direct coolant to pre-established directions. The tool may be installed automatically in a multipurpose machining center with no additional set up time required.