Patents by Inventor Stephen Kowalczykowski

Stephen Kowalczykowski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Multimers of single-stranded DNA-binding protein and methods of use thereof
    Patent number: 11186856
    Abstract: The invention provides multimers of S. solfataricus ssDNA binding protein that bind single stranded DNA. The multimers are robust and stable reagents for use in PCR and other techniques for engineering DNA. The invention further provides methods for performing nucleic acid amplification and engineering using the multimers.
    Type: Grant
    Filed: October 29, 2018
    Date of Patent: November 30, 2021
    Assignee: The Regents of the University of California
    Inventors: Cynthia A. Haseltine, Stephen Kowalczykowski
  • MULTIMERS OF S. SOLFATARICUS SINGLE-STRANDED DNA-BINDING PROTEIN AND METHODS OF USE THEREOF
    Publication number: 20190119714
    Abstract: The invention provides multimers of S. solfataricus ssDNA binding protein that bind single stranded DNA. The multimers are robust and stable reagents for use in PCR and other techniques for engineering DNA. The invention further provides methods for performing nucleic acid amplification and engineering using the multimers.
    Type: Application
    Filed: October 29, 2018
    Publication date: April 25, 2019
    Inventors: CYNTHIA A. HASELTINE, STEPHEN KOWALCZYKOWSKI
  • Expression and purification of fusion protein with multiple MBP tags
    Patent number: 9150897
    Abstract: The present invention provides a new method for recombinantly expressing a protein of interest, such as the human BRCA2 protein, BLM protein, CtIP protein, or EXOI protein, by expressing the protein in the form of a fusion protein comprising two maltose-binding protein (MBP) or glutathione-S-transferase (GST) tags. The expression cassette useful for this method and the fusion protein produced by this method are also described.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: October 6, 2015
    Assignee: The Regents of the University of California
    Inventors: Stephen Kowalczykowski, Ryan Jensen
  • EXPRESSION AND PURIFICATION OF FUSION PROTEIN WITH MULTIPLE MBP TAGS
    Publication number: 20130203116
    Abstract: The present invention provides a new method for recombinantly expressing a protein of interest, such as the human BR-CA2 protein, BLM protein, CtIP protein, or EXOI protein, by expressing the protein in the form of a fusion protein comprising two maltose-binding protein (MBP) or glutathione-S-transferase (GST) tags. The expression cassette useful for this method and the fusion protein produced by this method are also described.
    Type: Application
    Filed: August 5, 2011
    Publication date: August 8, 2013
    Applicant: The Regents of the University of California 1111 Franklin Street, 12th Floor
    Inventors: Stephen Kowalczykowski, Ryan Jensen
  • Single stranded DNA binding proteins from archaea and uses therefor
    Publication number: 20050164265
    Abstract: The invention provides ssDNA-binding proteins from three species of archaeons, Methanococcus jannaschii, Methanobacter theromoautotrophicum, and Archaeoglobus fulgidus, as well as the ability to identify ssDNA-binding proteins from other archaeons. The proteins help render DNA more accessible to DNA polymerase and are robust reagents for a variety of biotechnical processes, including PCR. The invention further provides nucleic acids encoding such proteins, vectors for transfecting host cells, host cells comprising the vectors, and methods of using the proteins.
    Type: Application
    Filed: January 14, 2005
    Publication date: July 28, 2005
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Stephen Kowalczykowski, Frederic Chedin, Erica Seitz