Patents by Inventor Stephen Levene

Stephen Levene has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240368584
    Abstract: Compositions and methods are provided for detection of dynamic loci in a genome, where such loci may comprise structural variations as a result of DNA recombination; DNA duplication, insertions, deletions, transpositions, and epigenetic changes. The methods may utilize microfluidic platforms and functionalized polymer matrices to allow determination of mechanisms of cell-type-specific, programmed genomic heterogeneity. The method and compositions allow determination of mechanisms of cell-type-specific, programmed genomic heterogeneity.
    Type: Application
    Filed: July 16, 2024
    Publication date: November 7, 2024
    Inventors: Massa Shoura, Andrew Z. Fire, Stephen Levene
  • Patent number: 12091660
    Abstract: Compositions and methods are provided for detection of dynamic loci in a genome, where such loci may comprise structural variations as a result of DNA recombination; DNA duplication, insertions, deletions, transpositions, and epigenetic changes. The methods may utilize microfluidic platforms and functionalized polymer matrices to allow determination of mechanisms of cell-type-specific, programmed genomic heterogeneity. The method and compositions allow determination of mechanisms of cell-type-specific, programmed genomic heterogeneity.
    Type: Grant
    Filed: April 10, 2023
    Date of Patent: September 17, 2024
    Assignees: The Board of Trustees of the Leland Stanford Junior University, Board of Regents, The University of Texas System
    Inventors: Massa Shoura, Andrew Z. Fire, Stephen Levene
  • Publication number: 20240218352
    Abstract: Methods are provided for the isolation and analysis of circular DNA from complex samples, based on the topology of the DNA molecule. A sample comprising DNA species is combined with a chaotropic dense salt solution. A fraction containing the circular DNA of interest is isolated and dialyzed to remove excess salt. In some embodiments salt gradients are generated by ultracentrifugation in the absence of intercalating dyes, e.g. ethidium bromide; and in the absence of protease digestion. The circular DNA thus isolated is substantially pure, e.g. greater than about 75%, greater than about 80%, greater than about 90%, greater than about 95% of DNA in the isolated fraction is comprised of circular DNA.
    Type: Application
    Filed: March 7, 2024
    Publication date: July 4, 2024
    Inventors: Massa Shoura, Stephen Levene, David Girata
  • Patent number: 11952570
    Abstract: Methods are provided for the isolation and analysis of circular DNA from complex samples, based on the topology of the DNA molecule. A sample comprising DNA species is combined with a chaotropic dense salt solution. A fraction containing the circular DNA of interest is isolated and dialyzed to remove excess salt. In some embodiments salt gradients are generated by ultracentrifugation in the absence of intercalating dyes, e.g. ethidium bromide; and in the absence of protease digestion. The circular DNA thus isolated is substantially pure, e.g. greater than about 75%, greater than about 80%, greater than about 90%, greater than about 95% of DNA in the isolated fraction is comprised of circular DNA.
    Type: Grant
    Filed: January 18, 2023
    Date of Patent: April 9, 2024
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Massa Shoura, Stephen Levene, David Girata
  • Publication number: 20230287397
    Abstract: Compositions and methods are provided for detection of dynamic loci in a genome. The methods may utilize microfluidic platforms and functionalized polymer matrices to allow determination of mechanisms of cell-type-specific, programmed genomic heterogeneity.
    Type: Application
    Filed: April 10, 2023
    Publication date: September 14, 2023
    Inventors: Massa Shoura, Andrew Z. Fire, Stephen Levene
  • Publication number: 20230151349
    Abstract: Methods are provided for the isolation and analysis of circular DNA from complex samples, based on the topology of the DNA molecule. A sample comprising DNA species is combined with a chaotropic dense salt solution. A fraction containing the circular DNA of interest is isolated and dialyzed to remove excess salt. In some embodiments salt gradients are generated by ultracentrifugation in the absence of intercalating dyes, e.g. ethidium bromide; and in the absence of protease digestion. The circular DNA thus isolated is substantially pure, e.g. greater than about 75%, greater than about 80%, greater than about 90%, greater than about 95% of DNA in the isolated fraction is comprised of circular DNA.
    Type: Application
    Filed: January 18, 2023
    Publication date: May 18, 2023
    Inventors: Massa Shoura, Stephen Levene, David Girata