Abstract: Described are methods and systems for enhanced dissociation of cells from a solid biological tissue sample. In some embodiments, a self-contained cartridge apparatus includes a first chamber for receiving the tissue sample, enables ultrasonic energy from a transducer assembly of a processing unit to dissociate cells from the sample in the first chamber, and collect viable cells of interest from an aqueous suspension in a second chamber fluidly connected to the first chamber via a channel. In some embodiments, to enhance dissociation of viable cells, a filter device includes a tubular body configured to be telescopically inserted into a container containing the tissue sample in an aqueous fluid. The filter device also includes a cell-filter mesh that covers a bottom opening of the tubular body and that is configured to compress the tissue sample to expel cells from the sample when the filter device is fully inserted into the container.

Abstract: The present invention provides an engine cooling method and apparatus. The engine cooling apparatus includes a cylinder block having a cylinder head mounted thereto. The cylinder block defines an inlet passage and a block jacket in fluid communication with the inlet passage. The cylinder head defines a lower cylinder head jacket in fluid communication with the block jacket, and an upper cylinder head jacket in fluid communication with the lower cylinder head jacket. The lower cylinder head jacket includes a plurality of nozzles configured to direct engine coolant and thereby provide cooling to portions of the cylinder head located near a plurality of exhaust ports, a plurality of injector ports, and a plurality of intake ports.