Patents by Inventor Stephen M. Hahn

Stephen M. Hahn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10941452
    Abstract: A method useful for identifying and isolating live circulating tumor cells is described. The method utilizes an adenoviral vector comprising a replication-competent adenovirus in which the E1 gene region is expressed under the control of a telomerase-specific promoter and further comprises a second expression cassette containing a marker protein, optionally fused to a detectable cell surface marker to permit detection of circulating tumor cells lacking cell surface markers. The method involves combining ex vivo a test sample from a patient suspected of having circulating tumor cells, an adenoviral probe system, and culture media for the cells. The test sample is incubated with the adenoviral system for a sufficient time to permit expression of the reporter protein. The marker gene expression can thereafter be quantitated and the marker-expressing cells may optionally be collected for further analysis.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: March 9, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Jay F. Dorsey, Gary D. Kao, Stephen M. Hahn
  • Patent number: 10338071
    Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5? and 3? ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3? ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample. Because the system is non-lytic, the testing can be repeated on the cells which remain viable in culture.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: July 2, 2019
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Stephen M. Hahn, Jay F. Dorsey, Gary D. Kao, Emigdio Reyes
  • Publication number: 20180113130
    Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5? and 3? ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3? ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample. Because the system is non-lytic, the testing can be repeated on the cells which remain viable in culture.
    Type: Application
    Filed: November 14, 2017
    Publication date: April 26, 2018
    Inventors: Stephen M. Hahn, Jay F. Dorsey, Gary D. Kao, Emigdio Reyes
  • Patent number: 9846157
    Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5? and 3? ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3? ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: December 19, 2017
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Stephen M. Hahn, Jay F. Dorsey, Gary D. Kao, Emigdio Reyes
  • Publication number: 20170298454
    Abstract: A method useful for identifying and isolating live circulating tumor cells is described. The method utilizes an adenoviral vector comprising a replication-competent adenovirus in which the E1 gene region is expressed under the control of a telomerase-specific promoter and further comprises a second expression cassette containing a marker protein, optionally fused to a detectable cell surface marker to permit detection of circulating tumor cells lacking cell surface markers. The method involves combining ex vivo a test sample from a patient suspected of having circulating tumor cells, an adenoviral probe system, and culture media for the cells. The test sample is incubated with the adenoviral system for a sufficient time to permit expression of the reporter protein. The marker gene expression can thereafter be quantitated and the marker-expressing cells may optionally be collected for further analysis.
    Type: Application
    Filed: October 5, 2015
    Publication date: October 19, 2017
    Inventors: Jay F. Dorsey, Gary D. Kao, Stephen M. Hahn
  • Publication number: 20150285786
    Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5? and 3? ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3? ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 8, 2015
    Inventors: Stephen M. Hahn, Jay F. Dorsey, Gary D. Kao, Emigdio Reyes
  • Patent number: 8082015
    Abstract: An embodiment of the invention includes a device, system and method for determining the characteristics of deep tissue. The novel method includes measuring blood flow rate and oxygenation characteristics of the tissue, and determining oxygen metabolism of the tissue as a function of the measure blood flow rate and measure oxygenation. The blood flow rate characteristics are measured as a function of light fluctuations caused by the tissue, while the oxygenation characteristics are measured as a function of transmission of light through the tissue with respect to the wavelength of light. The tissue may be layered tissue, for example, a portion of a brain. The tissue characteristics may be measured during times of varying levels of exercise intensity.
    Type: Grant
    Filed: April 13, 2005
    Date of Patent: December 20, 2011
    Assignee: The Trustees Of The University Of Pennsylvania
    Inventors: Arjun G. Yodh, Joel H. Greenberg, Guoqiang Yu, John A Detre, Turgut Durduran, Mark G. Burnett, Emile R. Mohler, III, Harry Quon, Stephen M. Hahn