Abstract: A cryogenic freezing vial. The vial includes a hollow sample container having exterior threads seated inside a hollow outer container having interior threads. The two containers define a surrounding volume there between which operates as a thermal barrier to resist temperature change within the sample container. A cap having a tubular wall extending outwardly from a continuous capping surface includes threads formed on both of the opposing sides of the tubular wall. The inner threaded side of the tubular wall can be screwed onto the hollow sample container, and then the outer threaded side of the tubular wall can be screwed into the hollow outer container. In such manner the cap is disposed in simultaneous threaded engagement between the two containers to intercouple and seal the containers from their surrounding environment.