Abstract: The present invention relates to apparatus and methods for quantitative protein design and optimization.

Abstract: The present invention relates to apparatus and methods for quantitative protein design and optimization.

Abstract: The instant invention provides methods, reagents, and computational tools for designing non-natural substrate analogs for enzymes, especially for designing unnatural amino acid analogs for aminoacyl tRNA Synthetases (AARSs), such as the Phe tRNA Synthetase. The instant invention also provides methods to incorporate unnatural amino acid analogs, especially those with interesting functional groups, into protein products to generate proteins of modified or novel functions.

Abstract: The present invention relates to apparatus and methods for quantitative protein design and optimization.

Abstract: The invention relates to novel proteins with novel integrin and I domain activity and nucleic acids encoding these proteins. The invention further relates to the use of the novel proteins in the treatment of integrin related disorders. TABLE 1 Computationally designed mutantsa WT ido1q ido1r ido2r jlm2r Backbone Energyb 1ido ?1037 ??1145 ?1138 ?1116 ??678 1jlm ?1059 +82758 ??840 ?1000 ?1086 Position Residues 139 I — — V — 153 M — — A — 156 F L W — — 157 V — — I — 160 V I — — — 199 V I I I — 215 I L L — V 219 V — — — I 223 F — — — L 238 V F F I I 239 V L L L — 240 I L L — — 259 A L L — — 269 I L — — — 271 V F — — — 287 I V V V — 299 V A I I — 308 I V — — — aMutants are named according to the structure that was stabilized (ido or jlm), the solvation potential used (1 or 2) and the definition of core residues (q or r).

Abstract: The present invention relates to apparatus and methods for quantitative protein design and optimization.

Abstract: The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying “structurally tolerant” residues of a polymer. Mutations of these, structurally tolerant residues are less likely to adversely affect desirable properties of a polymer sequence. The invention further provides improved methods for directed evolution wherein the structurally tolerant residues of a polymer are selectively mutated. Computer systems for implementing analytical methods of the invention are also provided.

Abstract: The invention relates to novel proteins with novel integrin and I domain activity and nucleic acids encoding these proteins. The invention further relates to the use of the novel proteins in the treatment of integrin related disorders.

Abstract: The present invention relates to an apparatus and method for quantitative protein design and optimization. In particular, the invention describes the use of Hybrid Exact Rotamer Optimization algorithms in protein design.

Abstract: The invention relates to the use of a variety of computational methods for generating enzyme-like protein catalysts. Specifically, computational methods are used to insert active site domains, including catalytic domains and binding domains, into a protein scaffold and optimize surrounding amino acids for interaction with the active site domain.

Abstract: The present invention relates to an apparatus and method for quantitative protein design and optimization. In particular, the invention describes the use of Hybrid Exact Rotamer Optimization algorithms in protein design.

Abstract: A micromachined, high Reynolds number, sub-millisecond liquid mixer for the study of chemical reaction kinetics. This bulk micromachined silicon mixer is capable of initiating and quenching chemical reactions in intervals as short as 100 .mu.s. The mixer chip contains two tee mixers connected by one channel which serves as a reaction chamber. Each tee mixer consists of opposing channels where liquids meet head-on and exit into a third channel forming the base of a "T".