Abstract: The preparation of water dispersible ceramide conjugates and derivatives of sphingolipid analogues is described. The conjugates and analogues are prepared by reacting a succinimidyl carbonate of a ?-Ala derivative with the primary amine of a functionalised spacer. Despite their dispersibility in water, the ceramide conjugates and derivatives of sphingolipid analogues spontaneously incorporate in to the plasma membranes of cells.

Abstract: The preparation of water dispersible ceramide conjugates and derivatives of sphingolipid analogues is described. The conjugates and analogues are prepared by reacting a succinimidyl carbonate of a ?-Ala derivative with the primary amine of a functionalised spacer. Despite their dispersibility in water, the ceramide conjugates and derivatives of sphingolipid analogues spontaneously incorporate into the plasma membranes of cells.

Abstract: Methods of treating an inert surface of a substrate to improve the adherence to the treated surface of micro-dimensioned particles including the steps of: contacting the inert surface with in an aqueous dispersion of a construct of the structure F-S-L; and then washing the surface with an aqueous vehicle to provide the treated surface, where F is a polyamine; S is —CO(CH2)2CO—, —CO(CH2)3CO—, —CO(CH2)4CO— or —CO(CH2)5CO—; and L is a diacyl- or dialkyl-glycerophospholipid.

Abstract: The preparation of water dispersible ceramide conjugates and derivatives of sphingolipid analogues is described. The conjugates and analogues are prepared by reacting a succinimidyl carbonate of a ?-Ala derivative with the primary amine of a functionalised spacer. Despite their dispersibility in water, the ceramide conjugates and derivatives of sphingolipid analogues spontaneously incorporate into the plasma membranes of cells.

Abstract: Methods of mitigating the risk of rejection of an allograft or xenograft in an incompatible recipient by neutralizing one or more populations of circulating antibody cross-reactive with a carbohydrate antigen expressed by the allograft or xenograft. The method includes administering to the recipient by intravenous infusion a concentration of a synthetic antigen lipid construct sufficient to neutralize the one or more populations of circulating antibody in the recipient.

Abstract: Water dispersible, multivalent ligand-lipid constructs that spontaneously and stably incorporate into membranes are disclosed.

Abstract: The invention relates to functional lipid constructs and their use in diagnostic and therapeutic applications, including serodiagnosis, where the functional moiety is carbohydrate, peptide, chemically reactive group, conjugator or fluorophore.

Abstract: Water dispersible, multivalent ligand-lipid constructs that spontaneously and stably incorporate into membranes are disclosed.

Abstract: Water dispersible, multivalent ligand-lipid constructs that spontaneously and stably incorporate into membranes are disclosed.

Abstract: A facile laboratory-based method and kit for use in accordance with the method is disclosed. The method allows for the localisation of biomolecules comprising a conjugatable sulfhydryl group to be localised to the surface of cells, such as red blood cells, as lipid conjugates. The method obviates the need to purify the lipid-conjugated biomolecule before contacting with the cells.

Abstract: Water dispersible, multivalent ligand-lipid constructs that spontaneously and stably incorporate into membranes are disclosed.

Abstract: Method of localizing cells to at least one discrete area on a surface of a substrate by propelling droplets of an aqueous dispersion of a synthetic construct of the structure F-S-L from a plurality of orifices located in a print head of an inkjet printer onto the surface. In the structure F-S-L, F is a functional moiety capable of associating with the cells by direct or indirect non-covalent interaction with biotin or an epitope of an antigen presented at the surface of the cells, S is a spacer selected to provide a construct that is dispersible in water at a temperature of 25° C. in the absence of organic solvents or detergents, and L is a diacyl- or dialkyl lipid.

Abstract: An antimicrobial surface treatment method comprising the step of contacting a stainless-steel surface with an aqueous dispersion of at least one functional-lipid construct where the lipid moiety is a phosphatidylethanolamine and the functional moiety confers the antimicrobial activity.

Abstract: Methods of treating an inert surface of a substrate to improve the adherence to the treated surface of micro-dimensioned particles including the steps of: contacting the inert surface with in an aqueous dispersion of a construct of the structure F-S-L; and then washing the surface with an aqueous vehicle to provide the treated surface, where F is a polyamine; S is —CO(CH2)2CO—, —CO(CH2)3CO—, —CO(CH2)4CO— or —CO(CH2)5CO—; and L is a diacyl- or dialkyl-glycerophospholipid.

Abstract: Biocompatible methods of functionalizing inert surfaces for use in biological applications are described. The methods employ the use of synthetic constructs of the generic structure F-S-L (where F is a functional moiety, S is a spacer selected to provide a construct that is dispersible in water, and L is a diacyl- or dialkyl-glycerophospholipid). An object of the invention is to localize or immobilize functional moieties to the inert surface of a substrate wherein the surface is comprised of glass, silver, polyamide, polycarbonate, polypropylene, polyethersulfone, polytetrafluoroethylene or polyvinylidene fluoride, and the substrate is comprised of a fiber, membrane, microsphere or nanosphere.

Abstract: Method of localizing cells to at least one discrete area on a surface of a substrate by propelling droplets of an aqueous dispersion of a synthetic construct of the structure F-S-L from a plurality of orifices located in a print head of an inkjet printer onto the surface. In the structure F-S-L, F is a functional moiety capable of associating with the cells by direct or indirect non-covalent interaction with biotin or an epitope of an antigen presented at the surface of the cells, S is a spacer selected to provide a construct that is dispersible in water at a temperature of 25° C. in the absence of organic solvents or detergents, and L is a diacyl- or dialkyl lipid.

Abstract: Methods of neutralizing circulating antibody and mitigating the risk of clinically significant adverse responses to incompatible transfusions and transplantations are described. The methods comprise the administration to the subject of dispersible antigen-lipid constructs.

Abstract: Method of incorporating a water soluble construct of the structure L-S-F into the lipid bi-layer of cells by contacting a suspension of the cells with a solution of the construct at a concentration and for a time and temperature sufficient to allow the construct to insert into the lipid bi-layer. In the structure L-S-F, F is a peptide, S is a spacer containing an oligomer of ethylene glycol covalently linking F to L and the number of ethylene oxide repeats in the oligomer of ethylene glycol is 6 to 14, and L is a phosphatidylethanolamine.

Abstract: A facile laboratory-based method and kit for use in accordance with the method is disclosed. The method allows for the localisation of biomolecules comprising a conjugatable sulfhydryl group to be localised to the surface of cells, such as red blood cells, as lipid conjugates. The method obviates the need to purify the lipid-conjugated biomolecule before contacting with the cells.

Abstract: Method of localizing a functional moiety (F) to at least one discrete area on a surface of a substrate, by propelling droplets of an aqueous dispersion of a synthetic construct of the structure F-S-L from a plurality of orifices located in a print head of an inkjet printer onto the surface. In the structure F-S-L, S is a spacer selected to provide a construct that is dispersible in water at a temperature of 25° C. in the absence of organic solvents or detergents, L is a diacyl- or dialkyl lipid and the at least one discrete area is in the shape of a symbol readable by optical character recognition (OCR) apparatus or a pattern having a combination of indicia in which the synthetic construct is present at different densities.