Patents by Inventor Stephen Novak
Stephen Novak has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9150877Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from Brassica napus or Brassica bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.Type: GrantFiled: October 17, 2012Date of Patent: October 6, 2015Assignee: Dow AgroSciences LLCInventors: Manju Gupta, Sean Michael Russell, Liu Yin Shen, Sivarama Reddy Chennareddy, Jyoti Rout, Stephen Novak
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Publication number: 20150064790Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.Type: ApplicationFiled: November 11, 2014Publication date: March 5, 2015Inventors: Manju Gupta, Asha M. Palta, Stephen Novak, Fyodor Urnov, Sunita Gopalan
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Publication number: 20140373598Abstract: An improved device for simulating gravel impact damage on panels, such as protected and unprotected areas of painted vehicle panels, is disclosed. The gravel impact damage simulator includes an enclosure, a gravel inlet port having an extension spout, a motor-driven rotatable impeller below the inlet port, a downwardly angled baffle between the inlet port and the impeller, and a test panel slot through which a test panel may be inserted into the enclosure. During operation, gravel is introduced through the extension spout and inlet port. The gravel drops into the angled baffle, where it is directed toward the rotating impeller. The rotating impeller strikes the gravel and projects it toward the test panel.Type: ApplicationFiled: June 24, 2014Publication date: December 25, 2014Inventors: Emmanuel Hondroulis, James Hondroulis, Marian Giorgakis, Stephen Novak, Michael Tutchton, John Diamond
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Patent number: 8911943Abstract: The present invention is a method to identify unknown DNA sequences which flank known DNA sequences. The invention improves the accuracy, sensitivity, and reproducibility for determining unknown DNA sequences which flank a known DNA sequence. This claimed method can be deployed as a high throughput method to quickly and efficiently identify plant genomic chromosomal sequences which flank a transgene. Further analysis of these unknown sequences can be used to characterize the transgene insertion site for the identification of rearrangements, insertions and deletions which result from the integration of the transgene. In addition, analysis of the chromosomal flanking sequences can be used to identify the location of the transgene on the chromosome.Type: GrantFiled: April 5, 2012Date of Patent: December 16, 2014Assignee: Dow AgroSciences, LLC.Inventors: Zehui Cao, Stephen Novak, Ning Zhou
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Patent number: 8889390Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.Type: GrantFiled: February 13, 2013Date of Patent: November 18, 2014Assignees: Dow AgroSciences LLC, Sangamo BioSciences, Inc.Inventors: Manju Gupta, Asha M. Palta, Stephen Novak, Fyodor Urnov, Sunita Gopalan
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Publication number: 20140157458Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.Type: ApplicationFiled: November 25, 2013Publication date: June 5, 2014Applicants: Sangamo BioSciences, Inc., Dow AgroSciences LLCInventors: Russell DeKelver, Manju Gupta, Jeffrey C. Miller, Stephen Novak, Joseph F. Petolino
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Patent number: 8685677Abstract: The invention relates in part to methods of detecting an AAD-12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD-12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention.Type: GrantFiled: November 24, 2010Date of Patent: April 1, 2014Assignee: Dow AgroSciences, LLC.Inventors: Stephen Novak, Yunxing C. Cui, Thomas W. Greene, Ning Zhou
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Publication number: 20140090113Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5? and 3? ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.Type: ApplicationFiled: September 6, 2013Publication date: March 27, 2014Applicant: Dow AgroSciences LLCInventors: Noel Cogan, John Forster, Matthew Hayden, Tim Sawbridge, German Spangenberg, Steven R. Webb, Manju Gupta, W. Mike Ainley, Matthew J. Henry, John Mason, Sandeep Kumar, Stephen Novak
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Publication number: 20130338006Abstract: This invention relates to soybean event pDAB8291.45.36.2, which includes a novel expression cassette comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding, and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to detection methods, including endpoint TaqMan PCR assays, for the detection of Event pDAB8291.45.36.2 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.Type: ApplicationFiled: December 2, 2011Publication date: December 19, 2013Applicants: MS TECHNOLOGIES, LLC, DOW AGROSCIENCES LLCInventors: Yunxing Cory Cui, Thomas Hoffman, Ning Zhou, Stephen Novak, Julissa Colon, Dawn M. Parkhurst, Sandra Grace Toledo, Terry R. Wright, Sean Michael Russell, Bruce Held, Vaithilingam Sekar
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Patent number: 8592645Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.Type: GrantFiled: October 22, 2010Date of Patent: November 26, 2013Assignees: Dow AgroSciences LLC, Sangamo BioSciences, Inc.Inventors: Russell DeKelver, Manju Gupta, Jeffrey C. Miller, Stephen Novak, Joseph F. Petolino
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Patent number: 8399218Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.Type: GrantFiled: September 25, 2008Date of Patent: March 19, 2013Assignees: Dow AgroSciences, LLC, Sangamo Bio Sciences, Inc.Inventors: Manju Gupta, Asha M. Palta, Stephen Novak, Fyodor Urnov, Sunita Gopalan
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Publication number: 20130029329Abstract: The invention relates in part to methods of detecting an AAD-12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD-12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention.Type: ApplicationFiled: November 24, 2010Publication date: January 31, 2013Applicant: DOW AGROSCIENCES LLCInventors: Stephen Novak, Yunxing Cory Cui, Thomas W. Greence, Ning Zhou
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Publication number: 20120258867Abstract: The present invention is a method to identify unknown DNA sequences which flank known DNA sequences. The invention improves the accuracy, sensitivity, and reproducibility for determining unknown DNA sequences which flank a known DNA sequence. This claimed method can be deployed as a high throughput method to quickly and efficiently identify plant genomic chromosomal sequences which flank a transgene. Further analysis of these unknown sequences can be used to characterize the transgene insertion site for the identification of rearrangements, insertions and deletions which result from the integration of the transgene. In addition, analysis of the chromosomal flanking sequences can be used to identify the location of the transgene on the chromosome.Type: ApplicationFiled: April 5, 2012Publication date: October 11, 2012Applicant: DOW AGROSCIENCES LLCInventors: ZEHUI CAO, Stephen Novak, Ning Zhou
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Publication number: 20120244533Abstract: This invention relates in part to detecting herbicide tolerant plants—more specifically, an aad-1 transformation event in corn plants. The subject invention also provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). Kits and conditions useful in conducting the assays are also provided. The subject invention also relates in part to plant breeding using the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. More specifically, the invention relates in part to an endpoint TaqMan PCR assay for AAD-1 corn event 40278-9. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the use of a preferred reference gene for use in determining zygosity.Type: ApplicationFiled: August 18, 2010Publication date: September 27, 2012Applicant: DOW AGROSCIENCES LLCInventors: Ning Zhou, Stephen Novak, Yunxing C. Cui, Thomas W. Greene
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Publication number: 20110167521Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.Type: ApplicationFiled: October 22, 2010Publication date: July 7, 2011Inventors: Russell DeKelver, Manju Gupta, Jeffrey C. Miller, Stephen Novak, Joseph F. Petolino
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Publication number: 20110151441Abstract: A method for zygosity analysis of the maize Cry1F event TC1507 is provided. The method provides TC1507 event-specific and maize endogenous reference gene-specific primers and TaqMan probe combinations for use in an endpoint biplex TaqMan PCR assay capable of producing robust genotype calls for assisting in molecular breeding of TC1507.Type: ApplicationFiled: December 18, 2009Publication date: June 23, 2011Applicant: Dow AgroSciences LLCInventors: Wei Chen, Wesley Marchione, Stephen Novak, Manju Gupta, Thomas W. Greene, Siva Kumptla
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Publication number: 20090205083Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.Type: ApplicationFiled: September 25, 2008Publication date: August 13, 2009Inventors: Manju Gupta, Asha M. Palta, Stephen Novak, Fyodor Urnov, Sunita Gopalan
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Publication number: 20070047538Abstract: A wireless computer system (30) is formed to have a host section (31) and a wireless hardware section (40). A first portion of a transmission frame is formed in system memory (36) of a host section (31) and a second portion of the transmission frame is formed in the wireless hardware section (40). The wireless hardware section (40) begins transmitting the first transmission frame portion while downloading the second transmission frame portion from the system memory (36) into the wireless hardware section (40).Type: ApplicationFiled: November 1, 2006Publication date: March 1, 2007Inventors: Stephan Rosner, William Kern, Ralf Flemming, Matthias Baer, Stephen Novak
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Publication number: 20020117020Abstract: A camshaft that has a hollow inner chamber and a number of outlet ports. One end of the camshaft is sealed. The other end of the camshaft connects to an engine at a bearing oil gallery. Here, oil is forced into the hollow shaft. A number of holes are drilled around the cam lobes. Two holes are on one side of the shaft and a third is on the opposite side of the shaft. These holes communicate with the hollow interior of the camshaft. As oil is pumped into the shaft, it is forced out of the holes and around the camshaft and the cam lobes. Because each cam lob has these oiling holes, lubrication is provided to all lobes simultaneously. Because the cam lobes are what wears, delivering oil to all lobes simultaneously increases the lobe life significantly.Type: ApplicationFiled: February 28, 2001Publication date: August 29, 2002Inventor: Stephen Novak