Patents by Inventor Stephen P. Chambers

Stephen P. Chambers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Dual expression vector system and screening methods
    Patent number: 8101378
    Abstract: The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to express a polynucleotide inserted at a downstream cloning site. In preferred embodiments the vector is used to derive a recombinant baculovirus that is used to infect host cells. In particular vectors the promoters are a baculovirus polh promoter and a T7lac promoter. In particular vectors the promoter effective for expression in bacteria is positioned between the promoter effective for expression in insect cells and a cloning site. The invention also features various high throughput screening methods.
    Type: Grant
    Filed: January 19, 2009
    Date of Patent: January 24, 2012
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventor: Stephen P. Chambers
  • DUAL EXPRESSION VECTOR SYSTEM AND SCREENING METHODS
    Publication number: 20100248304
    Abstract: The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to express a polynucleotide inserted at a downstream cloning site. In preferred embodiments the vector is used to derive a recombinant baculovirus that is used to infect host cells. In particular vectors the promoters are a baculovirus polh promoter and a T7lac promoter. In particular vectors the promoter effective for expression in bacteria is positioned between the promoter effective for expression in insect cells and a cloning site. The invention also features various high throughput screening methods.
    Type: Application
    Filed: January 19, 2009
    Publication date: September 30, 2010
    Applicant: VERTEX PHARMACEUTICALS INCORPORATED
    Inventor: STEPHEN P. CHAMBERS
  • Dual expression vector system and screening methods
    Patent number: 7498166
    Abstract: The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to express a polynucleotide inserted at a downstream cloning site. In preferred embodiments the vector is used to derive a recombinant baculovirus that is used to infect host cells. In particular vectors the promoters are a baculovirus polh promoter and a T7lac promoter. In particular vectors the promoter effective for expression in bacteria is positioned between the promoter effective for expression in insect cells and a cloning site. The invention also features various high throughput screening methods.
    Type: Grant
    Filed: January 12, 2005
    Date of Patent: March 3, 2009
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventor: Stephen P. Chambers