Patents by Inventor Stephen Paul Shannon

Stephen Paul Shannon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cytotoxicity test method for medical devices
    Patent number: 11378570
    Abstract: A cytotoxicity method for medical devices that has equivalent sensitivity, and greater efficiency compared to the colony formation assay (CFA) direct contact method is developed. The quantitative, direct contact cytotoxicity test method uses Mouse lymphoma TK or human lymphoblastoid TK6 suspension cells for medical device safety assessment. The advantages of using the suspension cell assay over adherent V79-4 cells is that this approach is quantitative, automated, not susceptible to potential mechanical damage imparted by the overlying test materials, and avoids issues related to non-adherence of cells to the test material which might impact the interpretation of test results.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: July 5, 2022
    Assignee: Alcon Inc.
    Inventors: Xuemei Liu, Fan Zhang, Denise P. Rodeheaver, Ann Marie Wright, Charlon Tolliver, Jamie Michaelis Walker, Robert Edward Rose, Stephen Paul Shannon, Jeffrey Charles White
  • CYTOTOXICITY TEST METHOD FOR MEDICAL DEVICE
    Publication number: 20190390245
    Abstract: A new two-layer modified agar overlay cytotoxicity method for medical device safety assessment are developed. The new two layer modified agar overlay cytotoxicity method has equivalent sensitivity, and greater efficiency compared to the ISO/USP direct contact and agar overlay assays. Assays were carried out in 6-well microplates. Nutrient agar medium with a 0.5% agar concentration was used to provide a base nutrient layer to support cell growth, L929 cells mixed nutrient agar (0.33% agar) were seeded on top of the base agar and cytotoxicity was evaluated after 24 hours per USP. Results demonstrated the two layer modified agar overlay cytotoxicity assay performs as well as the ISO/USP direct contact method with distinct advantages.
    Type: Application
    Filed: June 25, 2019
    Publication date: December 26, 2019
    Inventors: Xuemei Liu, Charlon Tolliver, Denise Rodeheaver, Stephen Paul Shannon, Fan Zhang, Lisa Maddox Walker, Mercedes Salvador-Silva, Kenneth Keven Williams
  • Cytotoxicity Test Method for Medical Devices
    Publication number: 20180348205
    Abstract: A cytotoxicity method for medical devices that has equivalent sensitivity, and greater efficiency compared to the colony formation assay (CFA) direct contact method is developed. The quantitative, direct contact cytotoxicity test method uses Mouse lymphoma TK or human lymphoblastoid TK6 suspension cells for medical device safety assessment. The advantages of using the suspension cell assay over adherent V79-4 cells is that this approach is quantitative, automated, not susceptible to potential mechanical damage imparted by the overlying test materials, and avoids issues related to non-adherence of cells to the test material which might impact the interpretation of test results.
    Type: Application
    Filed: May 24, 2018
    Publication date: December 6, 2018
    Inventors: Xuemei Liu, Fan Zhang, Denise P. Rodeheaver, Ann Marie Wright, Charion Tolliver, Jamie Michaelis Walker, Robert Edward Rose, Stephen Paul Shannon, Jeffrey Charles White