Patents by Inventor Stephen R. Decker
Stephen R. Decker has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230242955Abstract: A range of Cel7 putative cellobiohydrolase genes were identified using genome mining and homologous sequence alignment to Cel7A from Trichoderma reesei. A representative subset of these genes from across a broad diversity of evolutionarily disparate sources were cloned and expressed in T. reesei using a constitutive promotor and a common secretion signal. The purified recombinant enzymes were tested for efficacy on various substrates. The top performers were subjected to structural studies and subsites likely to confer enhanced performance were predicted using homology modeling and comparisons of natural sequence diversity. Once identified, the subsites were genetically introduced individually and combinatorically into the best in class Cel7A backbone we have found to date and then expressed in T. reesei and tested. A triple mutant was determined to have the highest cellulase activity we have measured in a cellobiohydrolase to date.Type: ApplicationFiled: August 30, 2022Publication date: August 3, 2023Inventors: Michael E. HIMMEL, Stephen R. DECKER, Roman BRUNECKY, Venkataramanan SUBRAMANIAN, Brandon Christopher KNOTT, Vladimir Vladimirovich LUNIN, Todd VANDERWALL
-
Publication number: 20210252189Abstract: Formulations of cellulose nanofiber useful for osteoinduction are provided.Type: ApplicationFiled: August 20, 2019Publication date: August 19, 2021Inventors: Stephen R. DECKER, Peter N. CIESIELSKI, Chad J. RONHOLDT
-
Patent number: 10036051Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.Type: GrantFiled: May 12, 2017Date of Patent: July 31, 2018Assignee: Alliance for Sustainable Energy, LLCInventors: William S. Adney, Gregg T. Beckham, Eric Jarvis, Michael E. Himmel, Stephen R. Decker, Jeffrey G. Linger, Kara Podkaminer, John O. Baker, Larry Taylor, II, Qi Xu, Arjun Singh
-
Publication number: 20170247731Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.Type: ApplicationFiled: May 12, 2017Publication date: August 31, 2017Inventors: William S. ADNEY, Gregg T. BECKHAM, Eric JARVIS, Michael E. HIMMEL, Stephen R. DECKER, Jeffrey G. LINGER, Kara PODKAMINER, John O. BAKER, Larry TAYLOR, II, Qi XU, Arjun SINGH
-
Patent number: 9683248Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.Type: GrantFiled: December 17, 2012Date of Patent: June 20, 2017Assignee: Alliance for Sustainable Energy, LLCInventors: William S. Adney, Gregg T. Beckham, Eric Jarvis, Michael E. Himmel, Stephen R. Decker, Jeffrey G. Linger, Kara Podkaminer, John O. Baker, Larry Taylor, II, Qi Xu, Arjun Singh
-
Publication number: 20140322765Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.Type: ApplicationFiled: December 17, 2012Publication date: October 30, 2014Inventors: William S. Adney, Gregg T. Beckham, Eric Jarvis, Michael E. Himmel, Stephen R. Decker, Jeffrey G. Linger, Kara Podkaminer, John O. Baker, Larry Taylor, II, Qi Xu, Arjun Singh
-
Patent number: 8637293Abstract: Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide.Type: GrantFiled: May 19, 2008Date of Patent: January 28, 2014Assignee: Alliance for Sustainable Energy, LLCInventors: William S. Adney, Michael E. Himmel, Stephen R. Decker, Eric P. Knoshaug, Mark R. Nimlos, Michael F. Crowley, Tina Jeoh
-
Patent number: 8283150Abstract: Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.Type: GrantFiled: October 8, 2008Date of Patent: October 9, 2012Assignee: Alliance for Sustainable Energy, LLCInventors: William S. Adney, John O. Baker, Stephen R. Decker, Yat-Chen Chou, Michael E. Himmel, Shi-You Ding
-
Publication number: 20120040408Abstract: Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol are disclosed herein, along with plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols than are typically employed are also disclosed.Type: ApplicationFiled: June 22, 2009Publication date: February 16, 2012Inventors: Stephen R. Decker, Michael J. Selig, Roman Brunecky, Todd Vinzant, Michael E. Himmell, David Lee, Michael Blaylock
-
Publication number: 20100105570Abstract: The disclosure provides reactors for rapid pretreatment of multiple biomass samples in a simple, process-driven, high throughput screening assay. This disclosure also provides methods and systems for rapid, high-throughput pretreatment and subsequent enzyme hydrolysis testing of multiple biomass samples.Type: ApplicationFiled: October 9, 2009Publication date: April 29, 2010Applicants: ALLIANCE FOR SUSTAINABLE ENERGY, LLC, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Michael H. Studer, Charles E. Wyman, Melvin P. Tucker, Michael J. Selig, Roman Brunecky, Michael E. Himmel, Stephen R. Decker
-
Publication number: 20090162916Abstract: Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide.Type: ApplicationFiled: May 19, 2008Publication date: June 25, 2009Applicant: Alliance for Sustainable Energy, LLCInventors: William S. Adney, Michael E. Himmel, Stephen R. Decker, Eric P. Knoshaug, Mark R. Nimlos, Michael F. Crowley, Tina Jeoh
-
Publication number: 20090081762Abstract: Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.Type: ApplicationFiled: October 8, 2008Publication date: March 26, 2009Applicant: ALLIANCE FOR SUSTAINABLE ENERGY, LLCInventors: William S. Adney, John O. Baker, Stephen R. Decker, Yat-Chen Chou, Michael E. Himmel, Shi-You Ding
-
Patent number: 7449550Abstract: Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.Type: GrantFiled: February 27, 2003Date of Patent: November 11, 2008Assignee: Alliance For Sustainable Energy, LLCInventors: William S. Adney, John O. Baker, Stephen R. Decker, Yat-Chen Chou, Michael E. Himmel, Shi-You Ding
-
Patent number: 7393673Abstract: The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as Gux1. Gux1 has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified Gux1. Methods of making and using Gux1 polypeptides, including fusions, variants, and derivatives, are also disclosed.Type: GrantFiled: July 28, 2001Date of Patent: July 1, 2008Assignee: Midwest Research InstituteInventors: William S. Adney, Shi-You Ding, Todd B. Vinzant, Michael E. Himmel, Stephen R. Decker, Suzanne Lantz McCarter
-
Patent number: 7375197Abstract: The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.Type: GrantFiled: January 14, 2002Date of Patent: May 20, 2008Assignee: MidWest Research InstituteInventors: William S. Adney, Stephen R. Decker, Suzanne Mc Carter, John O. Baker, Raphael Nieves, Michael E. Himmel, Todd B. Vinzant
-
Patent number: 7059993Abstract: The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.Type: GrantFiled: July 28, 2001Date of Patent: June 13, 2006Assignee: Midwest Research InstituteInventors: Shi-You Ding, William S. Adney, Todd B. Vinzant, Michael E. Himmel, Stephen R. Decker
-
Publication number: 20030170861Abstract: A nucleic acid molecule having a nucleic acid sequence that encodes a linker region of exoglucanase, said nucleic acid sequence comprising the nucleic sequence 5′-GGCGGAAACCCGCCTGGCACCACC-3′.Type: ApplicationFiled: January 14, 2002Publication date: September 11, 2003Inventors: William S. Adney, Stephen R. Decker, Suzanne McCarter, John O. Baker, Rafael Nieves, Michael E. Himmel, Todd B. Vinzant
-
Publication number: 20030104522Abstract: The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.Type: ApplicationFiled: July 28, 2001Publication date: June 5, 2003Inventors: Shi-You Ding, William S. Adney, Todd B. Vinzant, Michael E. Himmel, Stephen R. Decker
-
Publication number: 20030096342Abstract: The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as Gux1. Gux1 has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified Gux1. Methods of making and using Gux1 polypeptides, including fusions, variants, and derivatives, are also disclosed.Type: ApplicationFiled: July 28, 2001Publication date: May 22, 2003Inventors: William S. Adney, Shi-You Ding, Todd B. Vinzant, Michael E. Himmel, Stephen R. Decker, Suzanne Lantz McCarter
-
Publication number: 20030054535Abstract: The invention provides a method for increasing the specific activity of a glycosyl hydrolase on a substrate, comprising replacing a hydrophobic surface binding amino acid of the hydrolase with a positively charged amino acid; and a method for increasing the specific activity of a glycosyl hydrolase on a substrate, comprising replacing an active site associated glycosyl-stabilizing amino acid of the hydrolase with an amino acid, the replacing amino acid not strongly retarding cellobiose from leaving the active site. The invention further provides mutant glycosyl hydrolases, which include Y245G, Y42R, and W82R.Type: ApplicationFiled: November 19, 2001Publication date: March 20, 2003Inventors: Michael E. Himmel, William S. Adney, John O. Baker, Todd B. Vinzant, Steven R. Thomas, Joshua Sakon, Stephen R. Decker